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Volume 28, Number 4—April 2022
Dispatch

In Vitro Confirmation of Artemisinin Resistance in Plasmodium falciparum from Patient Isolates, Southern Rwanda, 2019

Welmoed van LoonComments to Author , Rafael Oliveira, Clara Bergmann, Felix Habarugira, Jules Ndoli, Augustin Sendegeya, Claude Bayingana, and Frank P. Mockenhaupt
Author affiliations: Charité–Universitaetsmedizin, Berlin, Germany (W. van Loon, R. Oliveira, C. Bergmann, F.P. Mockenhaupt); University Teaching Hospital of Butare, Butare, Rwanda (F. Habarugira, J. Ndoli, A. Sendegeya); King Faisal Hospital, Kigali (A. Sendegeya); University of Rwanda, Kigali, Rwanda (C. Bayingana)

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Figure 1

RSA 0–3-hour postinvasion survival rates (%) of an artemisinin-susceptible, K13 WT Plasmodium falciparum strain (NF54) and 4 P. falciparum patient isolates from Rwanda with K13 mutations. Each data point represents the mean of triplicate experiments. Isolate growth rates were only considered for analysis if 72-hour growth rates exceeded 1.5× rates in the nonexposed controls. Indicated error bars display the mean + SE; dashed line indicates the 1% survival rate threshold used to define artemisinin resistance (1,6). K13, kelch 13; RSA, ring-stage susceptibility assay; WT, wild-type.

Figure 1. RSA 0–3-hour postinvasion survival rates (%) of an artemisinin-susceptible, K13 WT Plasmodium falciparum strain (NF54) and 4 P. falciparum patient isolates from Rwanda with K13 mutations. Each data point represents the mean of triplicate experiments. Isolate growth rates were only considered for analysis if 72-hour growth rates exceeded 1.5× rates in the nonexposed controls. Indicated error bars display the mean + SE; dashed line indicates the 1% survival rate threshold used to define artemisinin resistance (1,6). K13, kelch 13; RSA, ring-stage susceptibility assay; WT, wild-type.

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