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Volume 28, Number 6—June 2022
Research

Effects of Acute Dengue Infection on Sperm and Virus Clearance in Body Fluids of Men

Joffrey Mons, Dominique Mahé-Poiron, Jean-Michel Mansuy, Hélène Lheureux, Delphine Nigon, Nathalie Moinard, Safouane Hamdi, Christophe Pasquier, Nathalie Dejucq-Rainsford, and Louis BujanComments to Author 
Author affiliations: Groupe Hospitalier Sud Réunion, St. Pierre, France (J. Mons, H. Lheureux); Institut National de la Santé et de la Recherche Médicale, Rennes, France (D. Mahé-Poiron, N. Dejucq-Rainsford); Centre Hospitalier-universitaire, Toulouse, France (J.-M. Mansuy, N. Moinard, S. Hamdi, C. Pasquier, L. Bujan); Institut National de la Santé et de la Recherche Médicale, Toulouse-Montpellier, France (N. Moinard, S. Hamdi, L. Bujan)

Main Article

Figure 2

Immunofluorescent images from Vero E6 cells exposed to semen samples obtained at day 7 after symptom onset from volunteers from Réunion Island with dengue virus (DENV) infection in a study of virus clearance and effects on reproductive function. A) Seminal plasma (patient 6); B) whole semen cells (patient 3); C) cells from 40% fraction obtained after semen preparation (patient 6); D) DENV-1 infected Vero E6 cells (positive control) (multiplicity of infection 0.01 for 3 days); E) noninfected Vero E6 cells; F) Vero E6 cells inoculated with noninfected semen (negative controls). VeroE6 cells were inoculated with the semen fractions indicated from DENV-infected (A, B, C) or uninfected patients (E) and cultured for 7 days (first passage). Images were made after detection of DENV envelop protein (DENV-E) from a second passage on VeroE6 of culture supernatants collected after the first passage. Red indicates DENV-E, blue indicates DAPI staining. Scale bars indicate 20 μm.

Figure 2. Immunofluorescent images from Vero E6 cells exposed to semen samples obtained at day 7 after symptom onset from volunteers from Réunion Island with dengue virus (DENV) infection in a study of virus clearance and effects on reproductive function. A) Seminal plasma (patient 6); B) whole semen cells (patient 3); C) cells from 40% fraction obtained after semen preparation (patient 6); D) DENV-1 infected Vero E6 cells (positive control) (multiplicity of infection 0.01 for 3 days); E) noninfected Vero E6 cells; F) Vero E6 cells inoculated with noninfected semen (negative controls). VeroE6 cells were inoculated with the semen fractions indicated from DENV-infected (A, B, C) or uninfected patients (E) and cultured for 7 days (first passage). Images were made after detection of DENV envelop protein (DENV-E) from a second passage on VeroE6 of culture supernatants collected after the first passage. Red indicates DENV-E, blue indicates DAPI staining. Scale bars indicate 20 μm.

Main Article

Page created: March 16, 2022
Page updated: May 22, 2022
Page reviewed: May 22, 2022
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