The family Microascaceae includes genera Microascus and Scopulariopsis, opportunistic fungi that have caused respiratory infection associated with poor outcome and an attributable mortality rate of 85%–100% (1,2). Treatment of invasive Microascus infection is challenging because of its high resistance to available therapies. Olorofim, a reversible inhibitor of the enzyme dihyroorotate dehydrogenase, has shown in vitro activity against a variety of mold species, including azole-resistant Aspergillus (3,4) and Microascus spp. (5). We describe 3 cases of invasive Microascus respiratory infection in France that were treated with olorofim (Table). All patients gave informed consent for publication.

Case 1 occurred in a 17-year-old boy with unremarkable medical history who was found unconscious with inhalation pneumonia, bilateral hemopneumothorax, and bilateral thoracic drainage after falling from the top of a rice silo (Appendix Figure 1). On day 2, the patient underwent venovenous extracorporeal membrane oxygenation. On day 38, after 5 weeks of adapted antimicrobial treatment, thoracic computed tomography (CT) scan showed worsening of bilateral necrotizing pneumonia with abscess. Bronchoalveolar lavage (BAL) and several bronchial aspirations grew a restricted light-gray fungal colony (Appendix Figure 2), identified through the Paris National Reference Center as compatible with Microascus melanosporus; we initiated a combination of olorofim (180 mg 2×/d on day 1, followed by 90 mg 2×/d) and terbinafine (500 mg 2×/d) for 6 weeks (Appendix Table). Radiologic findings and general clinical status improved; we discontinued oxygen support after 2 weeks (day 73). The last CT scan showed complete healing of lung lesions (day 120). The patient was still alive 1 year later.

Figure

Figure. Macroscopic observation of endobronchial Microascus cirrosus lesion in patient in France with refractory microascus bronchopulmonary infection before (A) and after (B) olorofim treatment. Arrows indicate the lesion.

Case 2 occurred in a 61-year-old lung transplant recipient who sought care for respiratory deterioration and decline in respiratory function. He had recently received isavuconazole for bronchial colonization with Aspergillus flavus. Thoracic CT scan at admission showed a new alveolar consolidation in the left upper lobe (Appendix Figure 3); fibroscopy showed a recent-onset yellowish irregular lesion in the culminal bronchus (Figure, panel A). We isolated M. cirrosus from a culture of bronchial aspirate and BAL. We found no other disseminated lesions and retained the diagnosis of invasive pulmonary M. cirrosus infection. We initiated olorofim (90 mg 2×/d). We observed, as previously described (6), a moderate increase of both tacrolimus and everolimus blood through levels, which may have been caused by olorofim, a weak inhibitor of CYP 3A4. After 3 months of treatment, lung function slightly improved, CT scan showed a near-complete disappearance of the consolidation, and BAL culture was sterile. After 8 months of olorofim treatment, the endobronchial lesion was gone (Figure, panel B). M. cirrosus was found in BAL after 6 months of olorofim, but no more was cultured from BAL 7 months after treatment initiation. The patient was still being treated with olorofim at 9 months.

Case 3 occurred in a 65-year-old lung transplant recipient who sought care for dyspnea. He had experienced progressive decline of respiratory function and had a diagnosis of grade 3 bronchiolitis obliterative syndrome (BOS) linked to obstructive respiratory failure 6 years after transplant. He required permanent oxygen support. At admission, he received isavuconazole that continued for 3 months because of bronchial colonization with A. fumigatus. Thoracic CT scan results showed an unchanged pattern of BOS. Nevertheless, bronchial fibroscopy showed a new-onset bronchial lesion, necrotic and blackish in appearance, obstructing the origin of the culminal bronchus (Appendix Figure 4). We isolated M. cirrosus samples. Patient received a combination of oral terbinafine (500 mg 2×/d) and olorofim (180 mg 2×/d on day 1 followed by 90 mg 2×/d). After 3 months of treatment, bronchial fibroscopy showed an improvement of the bronchial lesion, and M. cirrosus was not found in respiratory specimens. The patient died from respiratory failure attributed to progression of BOS.

Use of olorofim for invasive Microascus spp. respiratory infection has not previously been reported with a successful outcome; previous studies were conducted in vitro (4). Miossec et al. (1) reported a series of 9 cases; all 9 patients had a medical history of stem cell or solid organ transplantation, and 8 died. The only survivor was a patient considered immunocompetent with no identified underlying conditions. A fatal Microascus sp. lung infection was previously published in a lung transplant recipient (6). Here, we report 2 lung transplant recipients infected with M. cirrosus, a ubiquitous mold isolated from soil and moist indoor environments (7). The third case we report was a young immunocompetent adult with no underlying conditions infected with M. melanosporus; his exposure by falling in a rice silo and sustaining serious injuries may explain the onset of opportunistic infection.

Microascus spp. and Scopulariopsis (8) exhibit a multidrug-resistant phenotype (9). Skóra et al. reported antifungal susceptibility results of several Microascus species and confirmed high resistance to ciclopirox, 5-fluorocytosine, amphotericin B, and azoles. However, among echinocandin, lower minimum effective concentrations for caspofungin were reported (10). The highest in vitro activity was observed with terbinafin (10); synergistic activity was observed against some Scopulariopsis strains (9). Wiederhold et al. reported promising activity of olorofim on Scopulariopsis spp. and Microascus spp. fungi (5), but no synergistic in vitro activity was reported between olorofim and terbinafine against Microascus spp.

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