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Volume 29, Number 2—February 2023
Research

Novel Species of Brucella Causing Human Brucellosis, French Guiana

Frédégonde About, Theo Pastre, Mathilde Boutrou, Alex Yahiaoui Martinez, Alessia Melzani, Sandrine Peugny, Céline Michaud, Sami Zouaoui, Thierry Carage, Vincent Sainte Rose, Magalie Demar, Jean-Philippe Lavigne, Félix Djossou, David O’Callaghan, Loïc Epelboin, and Anne KerielComments to Author 
Author affiliations: Centre Hospitalier de Cayenne Andrée Rosemon, Cayenne, French Guiana (F. About, M. Boutrou, A. Melzani, C. Michaud, V. Sainte Rose, M. Demar, F. Djossou, L. Epelboin); Centre Hospitalier de Kourou, Kourou, French Guiana (F. About, M. Boutrou, S. Zouaoui, T. Carage); Carémeau University Hospital de Nimes, Nimes, France (T. Pastre, A.Y. Martinez, J.-P. Lavigne, D. O’Callaghan, A. Keriel); Centre Hospitalier de l’Ouest Guyanais, Saint-Laurent du Maroni, French Guiana (S. Peugny); Université de Guyane, Cayenne (M. Demar, L. Epelboin); Université de Montpellier, Nimes (J.-P. Lavigne, D. O’Callaghan, A. Keriel); Institut National de la Santé et de la Recherche Médicale (INSERM), Nimes (D. O’Callaghan, A. Keriel); INSERM, Cayenne (L. Epelboin)

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Figure 2

PCR typing of newly discovered Brucella isolates obtained from 2 patients in French Guiana, 2020. Genomic DNA from bacterial isolates BRSO-2020-213 and BRSO-2021-230 (numbers in bold) were used as a template for Bruce-Ladder (5) (A) or Suis-Ladder (6) (B) multiplex PCR. The profiles obtained after agarose gel electrophoresis were compared with the profile of type strains 16M (Brucella melitensis), 2308 (B. abortus), 1330 (B. suis bv1), Thomsen (B. suis bv2), 686 (B. suis bv3), 40 (B. suis bv4), 513 (B. suis bv5), RM6/66 (B. canis), Rev1 (B. melitensis vaccine strain), F8/08–60 (B. papionis), CCM 4915 (B. microti), or 5K33 (B. neotomae). Molecular-weight size markers in bp are shown at left. bv, biovar; Ø, negative control.

Figure 2. PCR typing of newly discovered Brucella isolates obtained from 2 patients in French Guiana, 2020. Genomic DNA from bacterial isolates BRSO-2020-213 and BRSO-2021-230 (numbers in bold) were used as a template for Bruce-Ladder (5) (A) or Suis-Ladder (6) (B) multiplex PCR. The profiles obtained after agarose gel electrophoresis were compared with the profile of type strains 16M (Brucella melitensis), 2308 (B. abortus), 1330 (B. suis bv1), Thomsen (B. suis bv2), 686 (B. suis bv3), 40 (B. suis bv4), 513 (B. suis bv5), RM6/66 (B. canis), Rev1 (B. melitensis vaccine strain), F8/08–60 (B. papionis), CCM 4915 (B. microti), or 5K33 (B. neotomae). Molecular-weight size markers in bp are shown at left. bv, biovar; Ø, negative control.

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