Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 29, Number 2—February 2023
Research

Novel Species of Brucella Causing Human Brucellosis, French Guiana

Frédégonde About, Theo Pastre, Mathilde Boutrou, Alex Yahiaoui Martinez, Alessia Melzani, Sandrine Peugny, Céline Michaud, Sami Zouaoui, Thierry Carage, Vincent Sainte Rose, Magalie Demar, Jean-Philippe Lavigne, Félix Djossou, David O’Callaghan, Loïc Epelboin, and Anne KerielComments to Author 
Author affiliations: Centre Hospitalier de Cayenne Andrée Rosemon, Cayenne, French Guiana (F. About, M. Boutrou, A. Melzani, C. Michaud, V. Sainte Rose, M. Demar, F. Djossou, L. Epelboin); Centre Hospitalier de Kourou, Kourou, French Guiana (F. About, M. Boutrou, S. Zouaoui, T. Carage); Carémeau University Hospital de Nimes, Nimes, France (T. Pastre, A.Y. Martinez, J.-P. Lavigne, D. O’Callaghan, A. Keriel); Centre Hospitalier de l’Ouest Guyanais, Saint-Laurent du Maroni, French Guiana (S. Peugny); Université de Guyane, Cayenne (M. Demar, L. Epelboin); Université de Montpellier, Nimes (J.-P. Lavigne, D. O’Callaghan, A. Keriel); Institut National de la Santé et de la Recherche Médicale (INSERM), Nimes (D. O’Callaghan, A. Keriel); INSERM, Cayenne (L. Epelboin)

Main Article

Figure 2

PCR typing of newly discovered Brucella isolates obtained from 2 patients in French Guiana, 2020. Genomic DNA from bacterial isolates BRSO-2020-213 and BRSO-2021-230 (numbers in bold) were used as a template for Bruce-Ladder (5) (A) or Suis-Ladder (6) (B) multiplex PCR. The profiles obtained after agarose gel electrophoresis were compared with the profile of type strains 16M (Brucella melitensis), 2308 (B. abortus), 1330 (B. suis bv1), Thomsen (B. suis bv2), 686 (B. suis bv3), 40 (B. suis bv4), 513 (B. suis bv5), RM6/66 (B. canis), Rev1 (B. melitensis vaccine strain), F8/08–60 (B. papionis), CCM 4915 (B. microti), or 5K33 (B. neotomae). Molecular-weight size markers in bp are shown at left. bv, biovar; Ø, negative control.

Figure 2. PCR typing of newly discovered Brucella isolates obtained from 2 patients in French Guiana, 2020. Genomic DNA from bacterial isolates BRSO-2020-213 and BRSO-2021-230 (numbers in bold) were used as a template for Bruce-Ladder (5) (A) or Suis-Ladder (6) (B) multiplex PCR. The profiles obtained after agarose gel electrophoresis were compared with the profile of type strains 16M (Brucella melitensis), 2308 (B. abortus), 1330 (B. suis bv1), Thomsen (B. suis bv2), 686 (B. suis bv3), 40 (B. suis bv4), 513 (B. suis bv5), RM6/66 (B. canis), Rev1 (B. melitensis vaccine strain), F8/08–60 (B. papionis), CCM 4915 (B. microti), or 5K33 (B. neotomae). Molecular-weight size markers in bp are shown at left. bv, biovar; Ø, negative control.

Main Article

References
  1. Godfroid  J, Al Dahouk  S, Pappas  G, Roth  F, Matope  G, Muma  J, et al. A “One Health” surveillance and control of brucellosis in developing countries: moving away from improvisation. Comp Immunol Microbiol Infect Dis. 2013;36:241–8. DOIPubMedGoogle Scholar
  2. Moreno  E. Retrospective and prospective perspectives on zoonotic brucellosis. Front Microbiol. 2014;5:213. DOIPubMedGoogle Scholar
  3. Fredriksson-Ahomaa  M. Wild boar: a reservoir of foodborne zoonoses. Foodborne Pathog Dis. 2019;16:153–65. DOIPubMedGoogle Scholar
  4. González-Espinoza  G, Arce-Gorvel  V, Mémet  S, Gorvel  JP. Brucella: reservoirs and niches in animals and humans. Pathogens. 2021;10:1–21. DOIPubMedGoogle Scholar
  5. López-Goñi  I, García-Yoldi  D, Marín  CM, de Miguel  MJ, Muñoz  PM, Blasco  JM, et al. Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains. J Clin Microbiol. 2008;46:3484–7. DOIPubMedGoogle Scholar
  6. López-Goñi  I, García-Yoldi  D, Marín  CM, de Miguel  MJ, Barquero-Calvo  E, Guzmán-Verri  C, et al. New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis. Vet Microbiol. 2011;154:152–5. DOIPubMedGoogle Scholar
  7. Afgan  E, Baker  D, Batut  B, van den Beek  M, Bouvier  D, Cech  M, et al. The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update. Nucleic Acids Res. 2018;46(W1):W537–44. DOIPubMedGoogle Scholar
  8. Brettin  T, Davis  JJ, Disz  T, Edwards  RA, Gerdes  S, Olsen  GJ, et al. RASTtk: a modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes. Sci Rep. 2015;5:8365. DOIPubMedGoogle Scholar
  9. Boschiroli  ML, Ouahrani-Bettache  S, Foulongne  V, Michaux-Charachon  S, Bourg  G, Allardet-Servent  A, et al. Type IV secretion and Brucella virulence. Vet Microbiol. 2002;90:341–8. DOIPubMedGoogle Scholar
  10. Cloeckaert  A, Bernardet  N, Koylass  MS, Whatmore  AM, Zygmunt  MS. Novel IS711 chromosomal location useful for identification of marine mammal Brucella genotype ST27, which is associated with zoonotic infection. J Clin Microbiol. 2011;49:3954–9. DOIPubMedGoogle Scholar
  11. Eisenberg  T, Hamann  HP, Kaim  U, Schlez  K, Seeger  H, Schauerte  N, et al. Isolation of potentially novel Brucella spp. from frogs. Appl Environ Microbiol. 2012;78:3753–5. DOIPubMedGoogle Scholar
  12. Al Dahouk  S, Hofer  E, Tomaso  H, Vergnaud  G, Le Flèche  P, Cloeckaert  A, et al. Intraspecies biodiversity of the genetically homologous species Brucella microti. Appl Environ Microbiol. 2012;78:1534–43. DOIPubMedGoogle Scholar
  13. Douine  M, Mosnier  E, Le Hingrat  Q, Charpentier  C, Corlin  F, Hureau  L, et al. Illegal gold miners in French Guiana: a neglected population with poor health. BMC Public Health. 2017;18:23. DOIPubMedGoogle Scholar
  14. Epelboin  L, Eldin  C, Thill  P, de Santi  VP, Abboud  P, Walter  G, et al. Human Q fever on the Guiana Shield and Brazil: recent findings and remaining questions. Curr Trop Med Rep. 2021;8:173–82. DOIPubMedGoogle Scholar
  15. Schwartz  FW, Lee  S, Darrah  TH. A review of the scope of artisanal and small-scale mining worldwide, poverty, and the associated health impacts. Geohealth. 2021;5(1):e2020GH000325.
  16. Mesureur  J, Arend  S, Cellière  B, Courault  P, Cotte-Pattat  PJ, Totty  H, et al. A MALDI-TOF MS database with broad genus coverage for species-level identification of Brucella. PLoS Negl Trop Dis. 2018;12:e0006874. DOIPubMedGoogle Scholar
  17. Dereeper  A, Guignon  V, Blanc  G, Audic  S, Buffet  S, Chevenet  F, et al. Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res. 2008;36(Web Server):W465-9. DOIPubMedGoogle Scholar

Main Article

Page created: December 20, 2022
Page updated: January 21, 2023
Page reviewed: January 21, 2023
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external