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Volume 29, Number 2—February 2023
Research Letter

Relapsing Fever Caused by Borrelia lonestari after Tick Bite in Alabama, USA

Laia J. Vazquez Guillamet1, Grace E. Marx, William Benjamin, Peter Pappas, Nicole A.P. Lieberman, Kimo Bachiashvili, Sixto Leal, and Joshua A. LiebermanComments to Author 
Author affiliations: University of Alabama, Birmingham, Alabama, USA (L.J. Vazquez Guillamet, W. Benjamin, P. Pappas, K. Bachiashvili, S. Leal); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (G.E. Marx); University of Washington School of Medicine, Seattle, Washington, USA (N.A.P. Lieberman, J.A. Lieberman)

Main Article

Table

Summary of BLAST results for 16S rRNA gene sequences used to clinically identify bacteria found in the patient’s blood sample in study of relapsing fever caused by Borrelia lonestari after tick bite in Alabama, USA*

Species GenBank accession nos. % Identity† Confidence‡
B. lonestari AY166715, AY682921, U23211 100% Published
B. lonestari AY682920 99.79% Published
B. turicatae NC_008710, NZ_CP015629 98.38% RefSeq
B. parkeri NZ_CP005851 98.38% RefSeq
B. venezuelensis MG651649 98.38% Unpublished
B. coriaceae NR_114544, NR_121718, NZ_CP005745 98.38% Type
B. miyamotoi AB900817, LC164096, LC164108 98.38% Published
B. hermsii NC_010673, NR_102957 97.78% Type

*The V1/V2 hypervariable region of the 16S rRNA bacterial gene was amplified by PCR and BLAST (https://blast.ncbi.nlm.nih.gov) was used to align the patient’s sequences with reference sequences in GenBank. †Percentage identity with the 16S rRNA bacterial sequence from patient blood. ‡Confidence refers to level of curation for each reference sequence identified by using BLAST. Published indicates sequence record associated with peer-reviewed manuscript; RefSeq indicates records from curated GenBank RefSeq database (https://www.ncbi.nlm.nih.gov/refseq); Type indicates sequence from type material; unpublished indicates minimally curated direct submission to GenBank not associated with a peer-reviewed manuscript.

Main Article

1Current affiliation: Barcelona Institute for Global Health, Barcelona, Spain.

Page created: December 20, 2022
Page updated: January 21, 2023
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