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Volume 29, Number 2—February 2023
Dispatch

Candida auris Discovery through Community Wastewater Surveillance during Healthcare Outbreak, Nevada, USA, 2022

Alessandro RossiComments to Author , Jorge Chavez, Thomas Iverson, John Hergert, Kelly Oakeson, Nathan LaCross, Chidinma Njoku, Andrew Gorzalski, and Daniel Gerrity
Author affiliation: Author affiliations: Utah Department of Health and Human Services, Salt Lake City, Utah, USA (A. Rossi, J. Chavez, T. Iverson, J. Hergert, K. Oakeson, N. LaCross); Nevada Department of Health and Human Services, Las Vegas, Nevada, USA (C. Njoku); University of Nevada, Reno, Nevada, USA (A. Gorzalski); Southern Nevada Water Authority, Las Vegas (D. Gerrity).

Main Article

Figure 1

Culture-based isolation of Candida auris from wastewater, southern Nevada, USA, 2022, showing the effects of incubation temperature and fluconazole selection on competing organisms. A, B) Incubating the enrichment broth at 42°C (B) instead of 40°C (A) mitigated growth of competing filamentous fungi present in wastewater. C, D) Adding fluconazole to the salt Sabouraud dulcitol broth (SSDB) enrichment broth was essential for recovering C. auris from the southern Nevada wastewater samples. We plated 100 μL aliquots of inoculated enrichment broths incubated at 42°C after 2 days on HardyCHROM Candida medium (Hardy Diagnostics, https://hardydiagnostics.com) or BBL CHROMagar Candida medium (Becton, Dickinson, and Co., https://www.bd.com). Inoculation in SSDB without fluconazole resulted in overgrowth of an unidentified competing yeast (C), whereas inoculation in SSDB with fluconazole enabled isolation of C. auris colonies (D).

Figure 1. Culture-based isolation of Candida auris from wastewater, southern Nevada, USA, 2022, showing the effects of incubation temperature and fluconazole selection on competing organisms. A, B) Incubating the enrichment broth at 42°C (B) instead of 40°C (A) mitigated growth of competing filamentous fungi present in wastewater. C, D) Adding fluconazole to the salt Sabouraud dulcitol broth (SSDB) enrichment broth was essential for recovering C. auris from the southern Nevada wastewater samples. We plated 100 μL aliquots of inoculated enrichment broths incubated at 42°C after 2 days on HardyCHROM Candida medium (Hardy Diagnostics, https://hardydiagnostics.com) or BBL CHROMagar Candida medium (Becton, Dickinson, and Co., https://www.bd.com). Inoculation in SSDB without fluconazole resulted in overgrowth of an unidentified competing yeast (C), whereas inoculation in SSDB with fluconazole enabled isolation of C. auris colonies (D).

Main Article

Page created: December 07, 2022
Page updated: January 21, 2023
Page reviewed: January 21, 2023
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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