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Volume 29, Number 5—May 2023
Research Letter

Panton-Valentine Leukocidin–Positive CC398 MRSA in Urban Clinical Settings, the Netherlands

Jairo GooskensComments to Author , Marja M. Konstantinovski, Margriet E.M. Kraakman, Jayant S. Kalpoe, Nathalie D. van Burgel, Eric C.J. Claas, and Thijs Bosch
Author affiliations: Leiden University Medical Center, Leiden, the Netherlands (J. Gooskens, M.M. Konstantinovski, M.E.M. Kraakman, E.C.J. Claas); Regional Public Health Laboratory Kennemerland, Haarlem, the Netherlands (J.S. Kalpoe); Haga Ziekenhuis, The Hague, the Netherlands (N.D. van Burgel); National Institute for Public Health and the Environment, Bilthoven, the Netherlands (T. Bosch)

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Figure

Maximum-parsimony tree of Panton-Valentine leukocidin–positive CC398 MRSA detected in urban clinical settings, the Netherlands. Bold text indicates 3 HO-MRSA strains detected in this study, which we deposited in GenBank (accession nos. SRR21673410 for L2-P01, SRR21624599 for L2-P02, and SRR21673965 for L2-P03); those strains were compared with 27 CC398 Staphylococcus aureus strains from GenBank. The tree was constructed by using GenBank accession no. AM990992.1 as a reference strain, and then rooted and grouped into clades, as previously described (1). Scale bar indicates nucleotide substitutions per 100 sites based on a concatenated alignment of 3,878 high-quality single-nucleotide polymorphisms. HO-MRSA, human-origin MRSA; LA-MRSA, livestock-associated MRSA; MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-susceptible S. aureus; ST, sequence type.

Figure. Maximum-parsimony tree of Panton-Valentine leukocidin–positive CC398 MRSA detected in urban clinical settings, the Netherlands. Bold text indicates 3 HO-MRSA strains detected in this study, which we deposited in GenBank (accession nos. SRR21673410 for L2-P01, SRR21624599 for L2-P02, and SRR21673965 for L2-P03); those strains were compared with 27 CC398 Staphylococcus aureus strains from GenBank. The tree was constructed by using GenBank accession no. AM990992.1 as a reference strain, and then rooted and grouped into clades, as previously described (1). Scale bar indicates nucleotide substitutions per 100 sites based on a concatenated alignment of 3,878 high-quality single-nucleotide polymorphisms. HO-MRSA, human-origin MRSA; LA-MRSA, livestock-associated MRSA; MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-susceptible S. aureus; ST, sequence type.

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References
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