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Volume 30, Number 10—October 2024
Dispatch

Oropouche Fever, Cuba, May 2024

Ana Julia Benitez1, Mayling Alvarez1, Lissette Perez, Rosario Gravier, Silvia Serrano, Denelsys Milagro Hernandez, Melissa Maria Perez, Gladys Gutierrez-Bugallo, Yanet Martinez, Ariamys Companioni, Carilda Peña, Jose Raul de Armas, Dayana Couto, Iliovanys Betancourt I, Madelaine Rivera Sanchez, Sonia Resik, Vivian Kouri, and Maria G. GuzmanComments to Author 
Author affiliations: Pedro Kouri Tropical Medicine Institute, Havana, Cuba (A.J. Benitez, M. Alvarez, L. Perez, R. Gravier, S. Serrano, D.M. Hernandez, M. Perez, G. Gutierrez-Bugallo Y. Martinez, A. Companioni, S. Resik, V. Kouri, M.G. Guzman); Cuban Ministry of Health, Havana (C. Peña, J.R. de Armas, D. Couto, I. Betancourt, M. Rivera)

Main Article

Figure 1

Molecular phylogenetic analysis of Oropouche viruses isolated in Cuba (red bracket) and reference sequences. The evolutionary history was inferred by using the maximum-likelihood method based on the Kimura 2-parameter model to the small segment of Oropouche orthobunyavirus from 9 patients from Boniato, Songo La Maya, and Cienfuegos (PP921382, PP921383, PP921384, PP921385, PP921386, PP921387, PP921388, PP921389, PP921390) (14). The tree with the highest log likelihood (−2,403.4997) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained by applying the neighbor-joining method to a matrix of pairwise distances estimated by using the maximum composite likelihood approach. A discrete gamma distribution was used to model evolutionary rate differences among sites (5 categories [+G, parameter = 0.1407]). The tree is drawn to scale, with branch lengths measured in number of substitutions per site. The analysis involved 101 nt sequences deposited in GenBank (accession numbers shown) from the different outbreaks and genotypes of Oropouche virus in the Americas and the Caribbean region. All positions with <95% site coverage were eliminated (i.e., <5% alignment gaps, missing data, and ambiguous bases were allowed at any position) The final dataset contained 521 positions. Evolutionary analyses were conducted in MEGA6 (13).

Figure 1. Molecular phylogenetic analysis of Oropouche viruses isolated in Cuba (red bracket) and reference sequences. The evolutionary history was inferred by using the maximum-likelihood method based on the Kimura 2-parameter model to the small segment of Oropouche orthobunyavirus from 9 patients from Boniato, Songo La Maya, and Cienfuegos (PP921382, PP921383, PP921384, PP921385, PP921386, PP921387, PP921388, PP921389, PP921390) (14). The tree with the highest log likelihood (−2,403.4997) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained by applying the neighbor-joining method to a matrix of pairwise distances estimated by using the maximum composite likelihood approach. A discrete gamma distribution was used to model evolutionary rate differences among sites (5 categories [+G, parameter = 0.1407]). The tree is drawn to scale, with branch lengths measured in number of substitutions per site. The analysis involved 101 nt sequences deposited in GenBank (accession numbers shown) from the different outbreaks and genotypes of Oropouche virus in the Americas and the Caribbean region. All positions with <95% site coverage were eliminated (i.e., <5% alignment gaps, missing data, and ambiguous bases were allowed at any position) The final dataset contained 521 positions. Evolutionary analyses were conducted in MEGA6 (13).

Main Article

References
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1These authors contributed equally to this article.

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