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Volume 30, Number 12—December 2024
Dispatch

Experimental Infection of Reindeer with Jamestown Canyon Virus

Kayla J. BuhlerComments to Author , John Blake, Heather Fenton, Isaac H. Solomon, and Emily Jenkins
Author affiliation: Inland Norway University of Applied Sciences, Innlandet, Norway (K.J. Buhler); University of Saskatchewan, Saskatoon, Saskatchewan, Canada (K.J. Buhler, E. Jenkins); University of Alaska Fairbanks, Fairbanks, Alaska, USA (J. Blake); Australian Registry of Wildlife Health, Taronga Conservation Society, Mosman, New South Wales, Australia (H. Fenton); Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis (H. Fenton); Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts, USA (I.H. Solomon)

Main Article

Figure 3

Histopathology of samples from reindeer experimentally infected with JCV. Representative brain sections from naive control (RD985), naive infected (RD977), and superinfected (RD978) animals shows H&E-stained sections of cerebral cortex and subcortical white matter with minimal diagnostic abnormalities, negative JCV RNA ISH staining, scattered IBA1-positive microglia/macrophages, and mild-to-moderate astrocytosis highlighted by GFAP immunohistochemistry. JCV ISH naive control inset panel demonstrates positive cytoplasmic staining in a cortical neuron from a positive control case of fatal JCV encephalitis in a human. All images taken with 20× objective with exception of the JCV ISH inset, taken with 60× objective. GFAP, glial fibrillary acidic protein; H&E, hematoxylin and eosin; IBA, allograft inflammatory factor 1; ISH, in situ hybridization; JCV, Jamestown Canyon virus.

Figure 3. Histopathology of samples from reindeer experimentally infected with JCV. Representative brain sections from naive control (RD985), naive infected (RD977), and superinfected (RD978) animals shows H&E-stained sections of cerebral cortex and subcortical white matter with minimal diagnostic abnormalities, negative JCV RNA ISH staining, scattered IBA1-positive microglia/macrophages, and mild-to-moderate astrocytosis highlighted by GFAP immunohistochemistry. JCV ISH naive control inset panel demonstrates positive cytoplasmic staining in a cortical neuron from a positive control case of fatal JCV encephalitis in a human. All images taken with 20× objective with exception of the JCV ISH inset, taken with 60× objective. GFAP, glial fibrillary acidic protein; H&E, hematoxylin and eosin; IBA, allograft inflammatory factor 1; ISH, in situ hybridization; JCV, Jamestown Canyon virus.

Main Article

Page created: October 25, 2024
Page updated: November 26, 2024
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