Volume 30, Number 5—May 2024
Research Letter
Crimean-Congo Hemorrhagic Fever Virus in Ticks Collected from Cattle, Corsica, France, 2023
Figure
![Figure. Phylogenetic analysis of Crimean-Congo hemorrhagic fever virus in ticks collected from cattle, Corsica, France, 2023. Map at left shows locations of cattle from which ticks were collected at the slaughterhouses of Ponte Leccia in the north and Cuttoli-Cortichiatto in the south during 2022–2023. Phylogenetic trees show small (A), medium (B), and large (C) RNA segments CCHFV strains. Red font indicates strains detected from Corsica; other sequences are named by GenBank accession number, geographic origin, and sampling year. Evolutionary analyses were conducted in MEGA6 (https://www.megasoftware.net) after best model determination. The optimal tree is shown for each fragment. Trees were constructed using the maximum-likelihood method based on sequences on the small (Tamura-Nei model), medium (general time-reversible model), and large (Tamura-Nei model) segments of the virus. All positions with <95% sequence site coverage were eliminated (i.e., <5% alignment gaps, missing data, or ambiguous bases were allowed at any position [partial deletion option]). Results of bootstrap test (1,000 replicates) are shown next to the branches. Genotypes are indicated by Roman numerals (8) with the equivalent clade nomenclature (9): I, West Africa (Africa 1); II, Central Africa (Africa 2); III, South and West Africa (Africa 3); IV, Middle East/Asia, divided into 2 groups Asia 1 and Asia 2; V (Europe 1), Europe/Turkey (Europe 1); VI, Greece (Europe 2); VII (Europe 3). Scale bars indicate nucleotide substitutions per site. CCHFV, Crimean-Congo hemorrhagic fever virus; L-RNA, large segment of CCHFV RNA. Phylogenetic analysis of Crimean-Congo hemorrhagic fever virus in ticks collected from cattle, Corsica, France, 2023. Map at left shows locations of cattle from which ticks were collected at the slaughterhouses of Ponte Leccia in the north and Cuttoli-Cortichiatto in the south during 2022–2023. Phylogenetic trees show small (A), medium (B), and large (C) RNA segments CCHFV strains. Red font indicates strains detected from Corsica; other sequences are named by GenBank accession number, geographic origin, and sampling year. Evolutionary analyses were conducted in MEGA6 (https://www.megasoftware.net) after best model determination. The optimal tree is shown for each fragment. Trees were constructed using the maximum-likelihood method based on sequences on the small (Tamura-Nei model), medium (general time-reversible model), and large (Tamura-Nei model) segments of the virus. All positions with <95% sequence site coverage were eliminated (i.e., <5% alignment gaps, missing data, or ambiguous bases were allowed at any position [partial deletion option]). Results of bootstrap test (1,000 replicates) are shown next to the branches. Genotypes are indicated by Roman numerals (8) with the equivalent clade nomenclature (9): I, West Africa (Africa 1); II, Central Africa (Africa 2); III, South and West Africa (Africa 3); IV, Middle East/Asia, divided into 2 groups Asia 1 and Asia 2; V (Europe 1), Europe/Turkey (Europe 1); VI, Greece (Europe 2); VII (Europe 3). Scale bars indicate nucleotide substitutions per site. CCHFV, Crimean-Congo hemorrhagic fever virus; L-RNA, large segment of CCHFV RNA.](/eid/images/23-1742-F1.jpg)
Figure. Phylogenetic analysis of Crimean-Congo hemorrhagic fever virus in ticks collected from cattle, Corsica, France, 2023. Map at left shows locations of cattle from which ticks were collected at the slaughterhouses of Ponte Leccia in the north and Cuttoli-Cortichiatto in the south during 2022–2023. Phylogenetic trees show small (A), medium (B), and large (C) RNA segments CCHFV strains. Red font indicates strains detected from Corsica; other sequences are named by GenBank accession number, geographic origin, and sampling year. Evolutionary analyses were conducted in MEGA6 (https://www.megasoftware.net) after best model determination. The optimal tree is shown for each fragment. Trees were constructed using the maximum-likelihood method based on sequences on the small (Tamura-Nei model), medium (general time-reversible model), and large (Tamura-Nei model) segments of the virus. All positions with <95% sequence site coverage were eliminated (i.e., <5% alignment gaps, missing data, or ambiguous bases were allowed at any position [partial deletion option]). Results of bootstrap test (1,000 replicates) are shown next to the branches. Genotypes are indicated by Roman numerals (8) with the equivalent clade nomenclature (9): I, West Africa (Africa 1); II, Central Africa (Africa 2); III, South and West Africa (Africa 3); IV, Middle East/Asia, divided into 2 groups Asia 1 and Asia 2; V (Europe 1), Europe/Turkey (Europe 1); VI, Greece (Europe 2); VII (Europe 3). Scale bars indicate nucleotide substitutions per site. CCHFV, Crimean-Congo hemorrhagic fever virus; L-RNA, large segment of CCHFV RNA.
References
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