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Volume 31, Supplement—April 2025
SUPPLEMENT ISSUE
Supplement
Real-Time Use of Monkeypox Virus Genomic Surveillance, King County, Washington, USA, 2022–2024
Figure

Figure. Phylogenetic reference tree of clade IIb monkeypox virus sequences as of December 2023 from study of real-time use of monkeypox virus genomic surveillance, King County, Washington, USA, 2022–2024. The tree shows the viral strain of sublineage A.2.1 identified from a King County, Washington, resident in fall 2023 (in red) is highly diverged from the B.1 lineage. Colors correspond to the location of the case from which a viral isolate was sampled and are either a region, the United States, or King County, Washington, for the specific isolate of interest in this case report. Phylogenetic tree generated using Nextclade dataset for “Mpox virus (All Clades)” in Auspice v2.61.1 (https://github.com/nextstrain/auspice.us). The reference sequence used in the tree is the clade IIb National Center for Biotechnology Information reference sequence NC_063383.1. Data sourced from GenBank on December 8, 2023 (13). Sequences were downsampled by the Nextstrain team to ≈500 sequences with the goal of capturing monkeypox virus diversity across geography, collection dates, and lineages. The dataset is archived by Nextclade (https://github.com/nextstrain/Nextclade_data/tree/master/data_output/nextstrain/mpox/all-clades/2024-01-16--20-31-02Z). The figure is filtered to the clade IIb branch within the larger dataset. Branches are shown for all lineages within clade IIb, and specific nodes are shown for selected lineages A.2, A.2.1, B.1.1, B.1.2, and B.1.3. A table of the nodes with metadata is included in the Appendix.