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Volume 31, Supplement—May 2025

SUPPLEMENT ISSUE
Supplement

Real-Time Use of Monkeypox Virus Genomic Surveillance, King County, Washington, USA, 2022–2024

Kathryn M. LauComments to Author , Michaela Banks, Kaila Bryant, Joanie D. Lambert, Laura Marcela Torres, Stephanie M. Lunn, Cory Yun, Pavitra Roychoudhury, B. Ethan Nunley, Jaydee Sereewit, Alexander L. Greninger, Allison Black, Vance Kawakami, Sargis Pogosjans, Elysia Gonzales, and Eric J. Chow
Author affiliation: Public Health–Seattle & King County, Seattle, Washington, USA (K.M. Lau, M. Banks, K. Bryant, J.D. Lambert, V. Kawakami, S. Pogosjans, E. Gonzales, E.J. Chow); Washington State Department of Health, Olympia, Washington, USA (L.M. Torres, S.M. Lunn, C. Yun, A. Black); University of Washington School of Medicine, Seattle (P. Roychoudhury, B.E. Nunley, J. Sereewit, A.L. Greninger, E.J. Chow); Fred Hutchinson Cancer Research Center, Seattle (P. Roychoudhury, A.L. Greninger); University of Washington School of Public Health, Seattle (E.J. Chow)

Main Article

Figure

Phylogenetic reference tree of clade IIb monkeypox virus sequences as of December 2023 from study of real-time use of monkeypox virus genomic surveillance, King County, Washington, USA, 2022–2024. The tree shows the viral strain of sublineage A.2.1 identified from a King County, resident in fall 2023 (in red) is highly diverged from the B.1 lineage. Colors correspond to the location of the case from which a viral isolate was sampled and are either a region, the United States, or King County, for the specific isolate of interest in this case report. Phylogenetic tree generated using Nextclade dataset for “Mpox virus (All Clades)” in Auspice v2.61.1 (https://github.com/nextstrain/auspice.us). The reference sequence used in the tree is the clade IIb GenBank reference sequence (accession no. NC_063383.1). Data sourced from GenBank on December 8, 2023 (13). Sequences were downsampled by the Nextstrain team to ≈500 sequences with the goal of capturing monkeypox virus diversity across geography, collection dates, and lineages. The dataset is archived by Nextclade (https://github.com/nextstrain/Nextclade_data/tree/master/data_output/nextstrain/mpox/all-clades/2024-01-16--20-31-02Z). The figure is filtered to the clade IIb branch within the larger dataset. Branches are shown for all lineages within clade IIb, and specific nodes are shown for selected lineages A.2, A.2.1, B.1.1, B.1.2, and B.1.3. A table of the nodes with metadata is included in the Appendix.

Figure. Phylogenetic reference tree of clade IIb monkeypox virus sequences as of December 2023 from study of real-time use of monkeypox virus genomic surveillance, King County, Washington, USA, 2022–2024. The tree shows the viral strain of sublineage A.2.1 identified from a King County, resident in fall 2023 (in red) is highly diverged from the B.1 lineage. Colors correspond to the location of the case from which a viral isolate was sampled and are either a region, the United States, or King County, for the specific isolate of interest in this case report. Phylogenetic tree generated using Nextclade dataset for “Mpox virus (All Clades)” in Auspice v2.61.1 (https://github.com/nextstrain/auspice.us). The reference sequence used in the tree is the clade IIb GenBank reference sequence (accession no. NC_063383.1). Data sourced from GenBank on December 8, 2023 (13). Sequences were downsampled by the Nextstrain team to ≈500 sequences with the goal of capturing monkeypox virus diversity across geography, collection dates, and lineages. The dataset is archived by Nextclade (https://github.com/nextstrain/Nextclade_data/tree/master/data_output/nextstrain/mpox/all-clades/2024-01-16--20-31-02Z). The figure is filtered to the clade IIb branch within the larger dataset. Branches are shown for all lineages within clade IIb, and specific nodes are shown for selected lineages A.2, A.2.1, B.1.1, B.1.2, and B.1.3. A table of the nodes with metadata is included in the Appendix.

Main Article

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