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Volume 31, Number 4—April 2025
Dispatch
Reemergence of Brucella abortus, Israel, 2021
Figure 2

Figure 2. Minimum-spanning tree 7 study isolates and 986 public genomes in study of reemergence of Brucella abortus, Israel, 2021. Tree shows core-genome multilocus sequence typing analysis of 7 study isolates of B. abortus (5 bovine isolates, 1 clinical human isolate and 1 B19 vaccine strain) and 986 public genomes (Appendix 1 Table 3, using an ad hoc scheme of 2,460 loci (at 95% genome presence threshold). Genomes are color-coded by assigned clade (6). Purple outer rings indicate B. abortus reference genomes (7 biovars and S19 vaccine strain); red outer rinds indicate Isolates from Israel (7 study isolates, 2 public genomes). Node size is proportional to the number of genomes.
References
- Zilberman B, Motro Y, Sagi O, Kornspan D, Ben-Shimol S, Gdalevich M, et al. Genomic epidemiology of clinical Brucella melitensis isolates from southern Israel. Microorganisms. 2022;10:238. DOIPubMedGoogle Scholar
- Bardenstein S, Gibbs RE, Yagel Y, Motro Y, Moran-Gilad J. Brucellosis outbreak traced to commercially sold camel milk through whole-genome sequencing, Israel. Emerg Infect Dis. 2021;27:1728–31. DOIPubMedGoogle Scholar
- Bardenstein S, Grupel D, Blum SE, Motro Y, Moran-Gilad J. Public and animal health risks associated with spillover of Brucella melitensis into dairy farms. Microb Genom. 2023;9:
mgen001014 . DOIPubMedGoogle Scholar - Banai M. Insights into the problem of B. Melitensis and rationalizing a vaccination programme in Israel. Prilozi. 2010;31:167–80.PubMedGoogle Scholar
- Ministry of Agriculture and Food Security. Regulations for sampling cattle in dairy farms for brucellosis. 2018 [in Hebrew] [accessed 2024 Jul 6]. https://www.gov.il/BlobFolder/policy/moag-pro-088/he/procedure_digum_chalav_bakar.pdf
- Janke NR, Williamson CHD, Drees KP, Suárez-Esquivel M, Allen AR, Ladner JT, et al. Global phylogenomic diversity of Brucella abortus: spread of a dominant lineage. [cited 2024 Jul 6]. Front Microbiol. 2023;14:
1287046 . DOIPubMedGoogle Scholar - El-Diasty M, Wareth G, Melzer F, Mustafa S, Sprague LD, Neubauer H. Isolation of Brucella abortus and Brucella melitensis from seronegative cows is a serious impediment in brucellosis control. Vet Sci. 2018;5:28. DOIPubMedGoogle Scholar
- Alamian S, Dadar M. Brucella melitensis infection in dog: a critical issue in the control of brucellosis in ruminant farms. Comp Immunol Microbiol Infect Dis. 2020;73:
101554 . DOIPubMedGoogle Scholar - Forbes LB. Brucella abortus infection in 14 farm dogs. J Am Vet Med Assoc. 1990;196:911–6. DOIPubMedGoogle Scholar
- Wareth G, Melzer F, El-Diasty M, Schmoock G, Elbauomy E, Abdel-Hamid N, et al. Isolation of Brucella abortus from a dog and a cat confirms their biological role in re-emergence and dissemination of bovine brucellosis on dairy farms. Transbound Emerg Dis. 2017;64:e27–30. DOIPubMedGoogle Scholar
- Sabra A, el Masry B, Shaib H. A review of brucellosis: a recent major outbreak in Lebanon. J Environ Sci Public Health. 2021;5:56–76. DOIGoogle Scholar
- Abdulrazzak M, Alshaghel MM, Anadani R, Shabouk MB, Alhashemi M, Breim F, et al. Seroprevalence of brucellosis antibodies and associated risk factors among the hospitalized patient, Aleppo, Syria: a hospital-based cross-sectional study. Ann Med Surg (Lond). 2024;86:1887–94. DOIPubMedGoogle Scholar
- Abo-Shehada MN. Seroprevalence of Brucella species in equids in Jordan. Vet Rec. 2009;165:267–8. DOIPubMedGoogle Scholar
- Aljanazreh B, Alzatari K, Tamimi A, Alsaafeen MH, Hassouneh W, Ashhab Y. Brucellosis re-emergence after a decade of quiescence in Palestine, 2015-2017: A seroprevalence and molecular characterization study. Transbound Emerg Dis. 2022;69:e130–40. DOIPubMedGoogle Scholar
- Hikal AF, Wareth G, Khan A. Brucellosis: why is it eradicated from domestic livestock in the United States but not in the Nile River Basin countries? Ger J Microbiol. 2023;3:19–25. DOIGoogle Scholar
1These authors contributed equally to this article.