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Volume 5, Number 2—April 1999
Research

Rapid Molecular Genetic Subtyping of Serotype M1 Group A Streptococcus Strains

Nancy Hoe*, Kazumitsu Nakashima*, Diana Grigsby*, Xi Pan*, Shu Jun Dou*, Steven Naidich†, Marianne Garcia‡, Emily Kahn‡, David Bergmire-Sweat‡, and James M. Musser*Comments to Author 
Author affiliations: *Baylor College of Medicine, Houston, Texas, USA; †Naidich Space Laboratory, Inc., New York, New York, USA; ‡Texas Department of Health, Austin, Texas, USA

Main Article

Figure 2

Variation in the sic gene and Sic protein identified in M1 group A Streptococcus isolates characterized in the study. The figure is a compilation of variations found in the 15 distinct sic alleles in the sample. The numbers at the top of the figure refer to the nucleotide sequence position of a sic allele described in reference 6. Single-letter amino acid abbreviations are used. SRR, amino-terminal short repeat region; Roman numeral, short repeats I-V which recur in SRR; R2 and R3, tandem repeat

Figure 2. Variation in the sic gene and Sic protein identified in M1 group A Streptococcus isolates characterized in the study. The figure is a compilation of variations found in the 15 distinct sic alleles in the sample. The numbers at the top of the figure refer to the nucleotide sequence position of a sic allele described in reference 6. Single-letter amino acid abbreviations are used. SRR, amino-terminal short repeat region; Roman numeral, short repeats I-V which recur in SRR; R2 and R3, tandem repeats; MGAS strain, Musser Group A Streptococcus strain number; X, presence of polymorphism.

Main Article

References
  1. Musser  JM, Krause  RM. The revival of group A streptococcal diseases, with a commentary on staphylococcal toxic shock syndrome. In: Krause RM, editor. Emerging infections. San Diego: Academic Press; 1998. p. 185-218.
  2. Martin  DR, Single  LA. Molecular epidemiology of group A streptococcus M type 1 infections. J Infect Dis. 1993;167:1112–7.PubMedGoogle Scholar
  3. Musser  JM, Hauser  JM, Kim  MH, Schlievert  PM, Nelson  K, Selander  RK. Streptococcus pyogenes causing toxic-shock-like syndrome and other invasive diseases: clonal diversity and pyrogenic exotoxin expression. Proc Natl Acad Sci U S A. 1991;88:2668–72. DOIPubMedGoogle Scholar
  4. Norgren  M, Norrby  A, Holm  SE. Genetic diversity in T1M1 group A streptococci in relation to clinical outcome of infection. J Infect Dis. 1992;166:1014–20.PubMedGoogle Scholar
  5. Musser  JM, Kapur  V, Szeto  J, Pan  X, Swanson  DS, Martin  DR. Genetic diversity and relationships among Streptococcus pyogenes strains expressing serotype M1 protein: recent intercontinental spread of a subclone causing episodes of invasive disease. Infect Immun. 1995;63:994–1003.PubMedGoogle Scholar
  6. Akesson  P, Sjoholm  AG, Bjorck  L. Protein SIC, a novel extracellular protein of Streptococcus pyogenes interfering with complement function. J Biol Chem. 1996;271:1081–8. DOIPubMedGoogle Scholar
  7. Perea Mejia  LM, Stockbauer  KE, Pan  X, Cravioto  A, Musser  JM. Characterization of group A Streptococcus strains recovered from Mexican children with pharyngitis by automated DNA sequencing of virulence-related genes: unexpectedly large variation in the gene (sic) encoding a complement inhibiting protein. J Clin Microbiol. 1997;35:3220–4.PubMedGoogle Scholar
  8. Stockbauer  KE, Grigsby  D, Pan  X, Fu  Y-X, Perea Mejia  LM, Cravioto  A, Hypervariability generated by natural selection in an extracellular complement-inhibiting protein of serotype M1 strains of group A Streptococcus. Proc Natl Acad Sci U S A. 1998;95:3128–33. DOIPubMedGoogle Scholar
  9. Granlund  M, Oberg  L, Sellin  M, Norgren  M. Identification of a novel insertion element, IS1548, in group B streptococci, predominantly in strains causing endocarditis. J Infect Dis. 1998;177:967–76.PubMedGoogle Scholar
  10. Berge  A, Rasmussen  M, Bjorck  L. Identification of an insertion sequence located in a region encoding virulence factors of Streptococcus pyogenes. Infect Immun. 1998;66:3449–53.PubMedGoogle Scholar
  11. Whatmore  AM, Kapur  V, Sullivan  DJ, Musser  JM, Kehoe  MA. Non-congruent relationships between variation in emm gene sequences and the population genetic structure of group A streptococci. Mol Microbiol. 1994;14:619–31. DOIPubMedGoogle Scholar
  12. Harbaugh  MP, Podbielski  A, Hugl  S, Cleary  PP. Nucleotide substitutions and small-scale insertion produce size and antigenic variation in group A streptococcal M1 protein. Mol Microbiol. 1993;8:981–91. DOIPubMedGoogle Scholar
  13. Johnson  LP, Schlievert  PM. Group A streptococcal phage T12 carries the structural gene for pyrogenic exotoxin type A. Mol Gen Genet. 1984;194:52–6. DOIPubMedGoogle Scholar
  14. Musser  JM, Kapur  V, Kanjilal  S, Shah  U, Musher  DM, Barg  NL, Geographic and temporal distribution and molecular characterization of two highly pathogenic clones of Streptococcus pyogenes expressing allelic variants of pyrogenic exotoxin A (scarlet fever toxin). J Infect Dis. 1993;167:337–46.PubMedGoogle Scholar
  15. Alland  D, Kalkut  GE, Moss  AR, McAdam  RA, Hahn  JA, Bosworth  W, Transmission of tuberculosis in New York City. An analysis by DNA fingerprinting and conventional epidemiologic methods. N Engl J Med. 1994;330:1710–6. DOIPubMedGoogle Scholar
  16. van der Zee  A, Mooi  F, van Embden  J, Musser  J. Molecular evolution and host adaptation in Bordetella spp.: phylogenetic analysis using multilocus enzyme electrophoresis and typing with three insertion sequences. J Bacteriol. 1997;179:6609–17.PubMedGoogle Scholar
  17. Robinson  DA, Hollingshead  SK, Musser  JM, Parkinson  AJ, Briles  DE, Crain  MJ. The IS1167 insertion sequence is a phylogenetically informative marker among isolates of serotype 6B Streptococcus pneumoniae. J Mol Evol. 1998;47:222–9. DOIPubMedGoogle Scholar
  18. Lawrence  JG, Dykhuizen  DE, DuBose  RF, Hartl  DL. Phylogenetic analysis using insertion sequence fingerprinting in Escherichia coli. Mol Biol Evol. 1989;6:1–14.PubMedGoogle Scholar
  19. Stanley  J, Jones  CS, Threlfall  EJ. Evolutionary lines among Salmonella enteritidis phage types are identified by insertion sequence IS200 distribution. FEMS Microbiol Lett. 1991;66:83–9. DOIPubMedGoogle Scholar
  20. Suvorov  A, Ferretti  J. Physical and genetic chromosomal map of an M type 1 strain of Streptococcus pyogenes. J Bacteriol. 1996;178:5546–9.PubMedGoogle Scholar
  21. Groenen  PMA, Bunschoten  AE, van Soolingen  D, van Embden  JDA. Nature of DNA polymorphism in the direct repeat cluster of Mycobacterium tuberculosis; application for strain differentiation by a novel typing method. Mol Microbiol. 1993;10:1057–65. DOIPubMedGoogle Scholar
  22. Kamerbeek  J, Schouls  L, Kolk  A, van Agterveld  M, van Soolingen  D, Kuijper  S, Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol. 1997;35:907–14.PubMedGoogle Scholar
  23. Sreevatsan  S, Pan  X, Stockbauer  KE, Connell  ND, Kreiswirth  BN, Whittam  TS, Restricted structural gene polymorphism in the Mycobacterium tuberculosis complex indicates evolutionarily recent global dissemination. Proc Natl Acad Sci U S A. 1997;94:9869–74. DOIPubMedGoogle Scholar
  24. Musser  JM, Kapur  V, Peters  JE, Hendrix  CW, Drehner  D, Gackstetter  GD, Real-time molecular epidemiologic analysis of an outbreak of Streptococcus pyogenes invasive disease in US Air Force trainees. Arch Pathol Lab Med. 1994;118:128–33.PubMedGoogle Scholar
  25. Yeh  RW, Ponce de Leon  A, Agasino  CB, Hahn  JA, Daley  CL, Hopewell  PC, Stability of Mycobacterium tuberculosis DNA genotypes. J Infect Dis. 1998;177:1107–11. DOIPubMedGoogle Scholar
  26. Musser  JM, Gray  BM, Schlievert  PM, Pichichero  ME. Streptococcus pyogenes pharyngitis: characterization of strains by multilocus enzyme genotype, M and T protein serotype, and pyrogenic exotoxin gene probing. J Clin Microbiol. 1992;30:600–3.PubMedGoogle Scholar
  27. LaPenta  D, Rubens  C, Chi  E, Cleary  PP. Group A streptococci efficiently invade human respiratory epithelial cells. Proc Natl Acad Sci U S A. 1994;91:12115–9. DOIPubMedGoogle Scholar
  28. Cleary  PP, McLandsborough  L, Ikeda  L, Cue  D, Krawczak  J, Lam  H. High-frequency intracellular infection and erythrogenic toxin A expression undergo phase variation in M1 group A streptococci. Mol Microbiol. 1998;28:157–67. DOIPubMedGoogle Scholar
  29. Muotiala  A, Seppala  H, Huovinen  P, Vuopio-Varkila  J. Molecular comparison of group A streptococci of T1M1 serotype from invasive and noninvasive infections in Finland. J Infect Dis. 1997;175:392–9.PubMedGoogle Scholar
  30. Davies  DD, McGeer  A, Schwartz  B, Green  K, Cann  D, Simor  AE, Invasive group A streptococcal infections in Ontario, Canada. N Engl J Med. 1996;335:547–53. DOIPubMedGoogle Scholar
  31. Zurawski  CA, Bardsley  MS, Beall  B, Elliott  JA, Facklam  R, Schwartz  B, Invasive group A streptococcal disease in metropolitan Atlanta: a population-based assessment. Clin Infect Dis. 1998;27:150–7. DOIPubMedGoogle Scholar
  32. Hughes  MK, Hughes  AL. Natural selection on Plasmodium surface proteins. Mol Biochem Parasitol. 1995;71:99–113. DOIPubMedGoogle Scholar

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