Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 5, Number 3—June 1999
Synopsis

Adhesins as Targets for Vaccine Development

Theresa M. Wizemann, John E. Adamou, and Solomon LangermannComments to Author 
Author affiliations: MedImmune, Inc., Gaithersburg, Maryland, USA

Main Article

Table 1

Adhesins of gram-negative bacteria

Adhesin Strain Ligand Reference
Pili familya Hultgren (33)
PapG Escherichia coli Gala(1-4)Gal in globoseries of glycolipids
SfaS E. coli a-sialyl-2 3-b-galactose
FimH E. coli Mannose-oligosaccharides
HifE Haemophilus influenzae Sialylyganglioside-GM1
PrsG E. coli Gala(1-4)Gal in globoseries of glycolipids
MrkD Klebsiella pneumoniae Type V collagen
FHA Bordetella pertussis Sulfated sugars on cell-surface glycoconjugates Brennan (34)
Pertactin B. pertussis Integrins Brennan (34)
HMW1/HMW2 H. influenzae Human epithelial cells St. Geme (35)
Hia H. influenzae Human conjunctival cells Barenkamp (36)
Leb-binding adhesin Helicobacter pylori Fucosylated Leb histo-blood group antigens Ilver (37)

aRepresentative examples from the large family of pilus-associated adhesins. FHA, filamentous hemagglutinin; HMW, high molecular weight; Hia, H. influenzae adhesin

Main Article

References
  1. Hilleman  MR. Paper presented at the International Symposium on Recombinant Vectors in Vaccine Development; 1993 May 23; Albany, New York. [Nucleic Acids Technologies Foundation, sponsored by IABS, FDA, USDA/APHIS, and NIAID/NIH].
  2. Alexander  J, Fikes  J, Hoffman  S, Franke  E, Sacci  J, Appella  E, The optimization of helper T lymphocyte (HTL) function in vaccine development. Immunol Res. 1998;18:7992. DOIPubMedGoogle Scholar
  3. St Geme  JW III. Bacterial adhesins: determinants of microbial colonization and pathogenicity. Adv Pediatr. 1997;44:4372.PubMedGoogle Scholar
  4. McGhee  J, Mestecky  J, Dertzbaugh  MT, Eldridge  JH, Hirasawa  M, Kiyono  H. The mucosal immune system: from fundamental concepts to vaccine development. Vaccine. 1992;10:7588. DOIPubMedGoogle Scholar
  5. Langermann  S. New approaches to mucosal immunization. Semin Gastrointest Dis. 1996;7:128.PubMedGoogle Scholar
  6. O'Hagan  DT. Recent advances in vaccine adjuvants for systemic and mucosal administration. J Pharm Pharmacol. 1998;50:110.PubMedGoogle Scholar
  7. Mestecky  J, Michalek  SM, Moldoveanu  Z, Russell  MW. Routes of immunization and antigen delivery systems for optimal mucosal immune responses in humans. Behring Inst Mitt. 1997;98:3343.PubMedGoogle Scholar
  8. Beachey  EH. Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface. J Infect Dis. 1981;143:32545.PubMedGoogle Scholar
  9. Beachey  EH, Giampapa  CS, Abraham  SN. Bacterial adherence: adhesin receptor-mediated attachment of pathogenic bacteria to mucosal surfaces. Am Rev Respir Dis. 1988;138:S458.PubMedGoogle Scholar
  10. Ofek  I, Sharon  N. Adhesins as lectins: specificity and role in infection. Curr Top Microbiol Immunol. 1990;151:91113.PubMedGoogle Scholar
  11. Hultgren  SJ, Abraham  S, Capron  M, Falk  P, St. Geme  JW, Normark  S. Pilus and non-pilus bacterial adhesins: assembly and function in cell recognition. Cell. 1993;73:887901. DOIPubMedGoogle Scholar
  12. Jenkinson  HF, Lamont  RJ. Streptococcal adhesion and colonization. Crit Rev Oral Biol Med. 1997;8:175200. DOIPubMedGoogle Scholar
  13. Abraham  SN, Sun  D, Dale  JB, Beachey  EH. Conservation of the D-mannose-adhesion protein among type 1 fimbriated members of the family Enterobacteriacaea. Nature. 1988;:6824. DOIPubMedGoogle Scholar
  14. Sokurenko  EV, Courtney  HS, Ohman  DE, Klemm  P, Hasty  DL. FimH family of type 1 fimbrial adhesins: functional heterogeneity due to minor sequence variations among fimH genes. J Bacteriol. 1994;176:74855.PubMedGoogle Scholar
  15. Langermann  S, Palaszynski  S, Barnhart  M, Auguste  G, Pinkner  JS, Burlein  J, Prevention of mucosal Escherichia coli infection by FimH-based systemic vaccination. Science. 1997;276:60711. DOIPubMedGoogle Scholar
  16. Palaszynski  S, Pinkner  J, Leath  S, Barren  P, Auguste  CG, Burlein  J, Systemic immunization with conserved pilus-associated adhesins protects against mucosal infections. Dev Biol Stand. 1998;92:11722.PubMedGoogle Scholar
  17. Finlay  B, Falkow  S. Common themes in microbial pathogenicity. Microbiol Rev. 1989;53:21030.PubMedGoogle Scholar
  18. Hoepelman  AI, Tuomanen  EI. Consequences of microbial attachment: directing host cell functions with adhesins. Infect Immun. 1992;60:172933.PubMedGoogle Scholar
  19. Svanborg  C, Hedlund  M, Connell  H, Agace  W, Duane  RD, Nilsson  A, Bacterial adherence and mucosal cytokine responses. Receptors and transmembrane signaling. Ann N Y Acad Sci. 1996;797:17790. DOIPubMedGoogle Scholar
  20. Falkow  S. Invasion and intracellular sorting of bacteria: searching for bacterial genes expressed during host/pathogen interactions. J Clin Invest. 1997;100:23943. DOIPubMedGoogle Scholar
  21. Mecsas  JJ, Strauss  EJ. Molecular mechanisms of bacterial virulence: type III secretion and pathogenicity islands. Emerg Infect Dis. 1996;2:27088. DOIPubMedGoogle Scholar
  22. Zhang  JP, Normark  S. Induction and gene expression in Escherichia coli after pilus-mediated adherence. Science. 1996;273:12346. DOIPubMedGoogle Scholar
  23. Donnenberg  MS, Kaper  JB, Finlay  BB. Interactions between enteropathogenic Escherichia coli and host epithelial cells. Trends Microbiol. 1997;5:10914. DOIPubMedGoogle Scholar
  24. Kenny  B, DeVinney  R, Stein  M, Reinscheid  DJ, Frey  EA, Finlay  BB. Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells. Cell. 1997;91:51120. DOIPubMedGoogle Scholar
  25. Svanborg-Eden  C, Marild  S, Korhonen  TK. Adhesion inhibition by antibodies. Scand J Infect Dis. 1982;33:728.
  26. O'Hanley  P, Lark  D, Falkow  S, Schoolnik  G. Molecular basis of Escherichia coli colonization of the upper urinary tract in Balb/c mice. Gal-Gal pili immunization prevents Escherichia coli pyelonephritis in the Balb/c mouse model of human pyelonephritis. J Clin Invest. 1985;75:34760. DOIPubMedGoogle Scholar
  27. Pecha  B, Low  D, O'Hanley  P. Gal-Gal pili vaccines prevent pyelonephritis by piliated Escherichia coli in a murine model. Single component Gal-Gal pili vaccines prevent pyelonephritis by homologous and heterologous piliated E. coli strains. J Clin Invest. 1989;83:21028. DOIPubMedGoogle Scholar
  28. Moon  HW, Bunn  TO. Vaccines for preventing enterotoxigenic Escherichia coli infections in farm animals. Vaccine. 1993;11:21320. DOIPubMedGoogle Scholar
  29. Maurer  L, Orndorff  P. Identification and characterization of genes determining receptor binding and pilus length of Escherichia coli type 1 pili. J Bacteriol. 1987;169:6405.PubMedGoogle Scholar
  30. Hanson  MS, Brinton  CC Jr. Identification and characterization of the Escherichia coli type 1 pilus adhesin protein. Nature. 1988;322:2658. DOIGoogle Scholar
  31. Bock  K, Breimer  ME, Brignole  A, Hansson  GC, Karlsson  KA, Larson  G, Specificity of binding of a strain of uropathogenic Escherichia coli to Gal 1-4Gal-containing glycosphingolipids. J Biol Chem. 1985;260:854551.PubMedGoogle Scholar
  32. Stromberg  N, Marklund  BI, Lund  B, Ilver  D, Hamers  A, Gaastra  W, Host-specificity of uropathogenic Escherichia coli depends on differences in binding specificity to Gal 1-4Gal-containing isoreceptors. EMBO J. 1990;9:200110.PubMedGoogle Scholar
  33. Hultgren  SJ, Jones  CH. Utility of the immunoglobulin-like fold of chaperones in shaping organelles of attachment in pathogenic bacteria. American Society for Microbiology News 1195;61:457-64.
  34. Brennan  MJ, Shahin  RD. Pertussis antigens that abrogate bacterial adherence and elicit immunity. Am J Respir Crit Care Med. 1996;154:S1459.PubMedGoogle Scholar
  35. St. Geme  JW III. Progress towards a vaccine for nontypable Haemophilus influenzae. The Finnish Medical Society DUODECIM. Ann Med. 1996;28:317. DOIPubMedGoogle Scholar
  36. Barenkamp  SJ, St Geme  JW III. Identification of a second family of high-molecular-weight adhesion proteins expressed by non-typable Haemophilus influenzae. Mol Microbiol. 1996;19:121523. DOIPubMedGoogle Scholar
  37. Ilver  D, Arnqvist  A, Ogren  J, Frick  IM, Kersulyte  D, Incecik  ET, Helicobacter pylori adhesin binding fucosylated histo-blood group antigens revealed by retagging. Science. 1998;279:3737. DOIPubMedGoogle Scholar
  38. Roberts  JA. Tropism in bacterial infections: urinary tract infections. J Urol. 1996;156:15529. DOIPubMedGoogle Scholar
  39. Demuth  DR, Duan  Y, Brooks  W, Holmes  AR, McNab  R, Jenkinson  HF. Tandem genes encode cell-surface polypeptides SspA and SspB which mediate adhesion of the oral bacterium Streptococcus gordonii to human and bacterial receptors. Mol Microbiol. 1996;20:40313. DOIPubMedGoogle Scholar
  40. McNab  R, Jenkinson  HF, Loach  DM, Tannock  GW. Cell-surface-associated polypeptides CshA and CshB of high molecular mass are colonization determinants in the oral bacterium Streptococcus gordonii. Mol Microbiol. 1994;14:7435. DOIPubMedGoogle Scholar
  41. Jonsson  K, Signas  C, Muller  HP, Lindberg  M. Two different genes encode fibronectin binding proteins in Staphylococcus aureus. The complete nucleotide sequence and characterization of the second gene. Eur J Biochem. 1991;202:10418. DOIPubMedGoogle Scholar
  42. Talay  SR, Valentin-Weigand  P, Timmis  KN, Chhatwal  GS. Domain structure and conserved epitopes of Sfb protein, the fibronectin-binding protein adhesin of Streptococcus pyogenes. Mol Microbiol. 1994;13:5319. DOIPubMedGoogle Scholar
  43. Hanski  E, Caparon  M. Protein F, a fibronectin-binding protein, is an adhesin of the group A streptococcus Streptococcus pyogenes. Proc Natl Acad Sci U S A. 1992;89:61726. DOIPubMedGoogle Scholar
  44. Burnette-Curley  D, Wells  V, Viscount  H, Munro  CL, Fenno  JC, Fives-Taylor  P, FimA, a major virulence factor associated with Streptococcus parasanguis endocarditis. Infect Immun. 1995;63:466974.PubMedGoogle Scholar
  45. Rosenow  C, Ryan  P, Weiser  JN, Johnson  S, Fontan  P, Ortqvist  A, Contribution of novel choline-binding proteins to adherence, colonization and immunogenicity of Streptococcus pneumoniae. Mol Microbiol. 1997;25:81929. DOIPubMedGoogle Scholar
  46. Hammerschmidt  S, Talay  SR, Brandtzaeg  P, Chhatwal  GS. SpsA, a novel pneumococcal surface protein with specific binding to secretory immunoglobulin A and secretory component. Mol Microbiol. 1997;25:111324. DOIPubMedGoogle Scholar
  47. Briles  DE, Hollingshead  SK, Swiatlo  E, Brooks-Walter  A, Szalai  A, Virolainen  A, PspA and PspC: their potential for use as pneumococcal vaccines. Microb Drug Resist. 1997;3:4018. DOIPubMedGoogle Scholar
  48. Smith  BL, Cheng  Q, Hostetter  MK. Characterization of a pneumococcal surface protein that binds complement protein C3 and its role in adhesion [poster D-122]. The American Society for Microbiology (1998) 98th General Meeting; Atlanta, Georgia.
  49. Hajishengallis  G, Russell  MW, Michalek  SM. Comparison of an adherence domain and a structural region of Streptococcus mutans antigen I/II in protective immunity against dental caries in rats after intranasal immunization. Infect Immun. 1998;66:17403.PubMedGoogle Scholar
  50. Crowley  PJ, Brady  LJ, Piacentini  DA, Bleiweis  AS. Identification of a salivary agglutinin-binding domain within cell surface adhesin P1 of Streptococcus mutans. Infect Immun. 1993;61:154752.PubMedGoogle Scholar
  51. Todryk  SM, Kelly  CG, Lehner  T. Effect of route of immunisation and adjuvant on T and B cell epitope recognition within a streptococcal antigen. Vaccine. 1998;16:17480. DOIPubMedGoogle Scholar
  52. McNab  R, Holmes  AR, Clark  JM, Tannock  GW, Jenkinson  HF. Cell surface polypeptide CshA mediates binding of Streptococcus gordonii to other oral bacteria and to immobilized fibronectin. Infect Immun. 1996;64:420210.
  53. Schennings  T, Heimdahl  A, Coster  K, Flock  J-I. Immunization with fibronectin binding protein from Staphylococcus aureus protects against experimental endocarditis in rats. Microb Pathog. 1993;15:22736. DOIPubMedGoogle Scholar
  54. Lee  JC. The prospects for developing a vaccine against Staphylococcus aureus. Trends Microbiol. 1996;4:1626. DOIPubMedGoogle Scholar
  55. Molinari  G, Talay  SR, Valentin-Weigand  P, Rohde  M, Chhatwal  GS. The fibronectin-binding protein of Streptococcus pyogenes, SfbI, is involved in the internalization of group A streptococci by epithelial cells. Infect Immun. 1997;65:135763.PubMedGoogle Scholar
  56. Correia  FF, DiRienzo  JM, McKay  TL, Rosan  B. scbA from Streptococcus crista CC5A: an atypical member of the lraI gene family. Infect Immun. 1996;64:211421.PubMedGoogle Scholar
  57. Dintilhac  A, Alloing  G, Granadel  C, Claverys  JP. Competence and virulence of Streptococcus pneumoniae: Adc and PsaA mutants exhibit a requirement for Zn and Mn resulting from inactivation of putative ABC metal permeases. Mol Microbiol. 1997;25:72739. DOIPubMedGoogle Scholar
  58. Fenno  JC, Shaikh  A, Spatafora  G, Fives-Taylor  P. The fimA locus of Streptococcus parasanguis encodes an ATP-binding membrane transport system. Mol Microbiol. 1995;15:84963. DOIPubMedGoogle Scholar
  59. Talkington  DF, Brown  BG, Tharpe  JA, Koenig  A, Russell  H. Protection of mice against fatal pneumococcal challenge by immunization with pneumococcal surface adhesin A (PsaA). Microb Pathog. 1996;21:1722. DOIPubMedGoogle Scholar
  60. Berry  AM, Paton  JC. Sequence heterogenity of PsaA, a 37-kilodalton putative adhesin essential for virulence of Streptococcus pneumoniae. Infect Immun. 1996;64:525562.PubMedGoogle Scholar
  61. Novak  R, Brown  JS, Charpentier  E, Tuamonen  E. Penicillin tolerance genes of Streptococcus pneumoniae: the ABC-type manganese permease complex Psa. Mol Microbiol. 1998;29:128596. DOIPubMedGoogle Scholar
  62. Dormizter  M, Wizemann  TM, Adamou  JE, Walsh  W, Gayle  T, Langermann  S, Sequence and structural analysis of CbpA, a novel choline-binding protein of Streptococcus pneumoniae [poster B-3]. The American Society for Microbiology (1998) 98th General Meeting; Atlanta, Georgia.
  63. Schaeffer  AJ, Amundsen  SK, Schnidt  LN. Adherence of Escherichia coli to human urinary tract epithelial cells. Infect Immun. 1979;24:7539.PubMedGoogle Scholar
  64. Ofek  I, Mosek  A, Sharon  N. Mannose-specific adherence of Escherichia coli freshly excreted in the urine of patients with urinary tract infections, and of isolates subcultured from the infected urine. Infect Immun. 1981;34:70811.PubMedGoogle Scholar
  65. Connell  H, Agace  W, Klemm  P, Schembri  M, Marild  S, Svanborg  C. Type 1 fimbrial expression enhances Escherichia coli virulence for the urinary tract. Proc Natl Acad Sci U S A. 1996;93:982732. DOIPubMedGoogle Scholar
  66. Frey  A, Neutra  MR. Targeting of mucosal vaccines to Peyer's patch M cells. Behring Inst Mitt. 1997;98:37689.PubMedGoogle Scholar

Main Article

Page created: December 10, 2010
Page updated: December 10, 2010
Page reviewed: December 10, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external