Volume 5, Number 5—October 1999
Synopsis
Infections Associated with Eating Seed Sprouts: An International Concern
Table 2
Control of microorganisms in seed sprouts, by type of treatment and treatment results
Organism, origin | Treatment | Results of treatment | Ref. |
---|---|---|---|
Aerobic bacteria, rice seeds | 1,000 ppm NaOCl or 10,000 ppm H2O2 | 102 to 103 reductions in aerobic plate counts; germination inhibited | 32 |
Enterobacteriaceae, pseudomonads, commercial sprouts | Washing in water | Ineffective in removing bacteria | 33 |
Aerobic bacteria, mung bean sprouts | 100 ppm chlorine or 5,000 ppm chlorine | Reduced microflora by <1 log and 2 logs, respectively | 35 |
Salmonella Stanley, alfalfa seeds | Chlorine and hot water | No reduction at low levels; reduction of S. Stanley achieved with 2,040 ppm chlorine | 27 |
Salmonella, alfalfa seeds | 1,800 ppm Ca(OCl)2 or 2,000 ppm NaOCl or 6% H2O2 or 80% ethanol | Salmonella populations reduced by >3 logs, but pathogen not eliminated | 37 |
E. coli O157:H7, alfalfa seeds | 500, 1,000, or >2,000 ppm Ca(OCl)2; 500 ppm acidified ClO2; >100 ppm and 500 ppm acidified ClO2; 30% or 70% ethanol; >1% H2O2; 8% H2O2 for 10 min; dry storage | Populations reduced but not eliminated; germination decreased; pathogen unaffected by dry storage at 5ºC | 38 |
E. coli O157:H7, alfalfa seeds at various stages of sprouting | 2,000 ppm NaOCl; 200 and 2,000 ppm Ca(OCl)2; 500 ppm acidified ClO2 | Populations substantially reduced but not eliminated | 30 |
S. Stanley, alfalfa seeds | Heat, 54 to 71ºC | 54ºC for 5 min reduced population from 260 to 6-9 cfu/g; treatment for 10 min reduced viability of seed | 27 |
E. coli O157:H7, alfalfa seeds and sprouts | Irradiation at >1.0 kiloGray | Pathogen controlled without affecting germination | 39 |
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