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Volume 6, Number 5—October 2000

Toxin Gene Expression by Shiga Toxin-Producing Escherichia coli: the Role of Antibiotics and the Bacterial SOS Response

Patrick T. Kimmitt, Colin R. Harwood, and Michael R. BarerComments to Author 

Author affiliations: The Medical School, University of Newcastle, Newcastle upon Tyne, United Kingdom

Article in Chinese

Main Article


Induction of stx2 expression by antibiotics under different incubation conditionsa

Target of action Incubation conditionsb
Agent (disc content mg/L) mO2/37 O2/37 mO2/42 AnO2/37 O2/30 O2/42 Zone patternc
Background + - - - + - NA
DNA Gyrase
Quinolonesd + + + + + +/- >MIC
Novobiocin (30) + - - - + - >MIC
Folate metabolism
Trimethoprim (5) + + - + + - >MIC
Smx (25) + + - + - - >MIC
Metronidazole (50) - - - - - - NA
Furazolidone (50) + + - - + - =MIC
Cell envelope
Cephalexin (30) + + - - +/- - =MIC
Amoxycillin (25) + + - - - - =MIC
Amoxy/Clav (20/10) +/- - - - - - =MIC
Ampicillin (10) + + - - - - =MIC
Pip/Taz (10/75) + + - - - - =MIC
Imipenem (10) - - - - - - NA
Aztreonam (30) + + - - - - =MIC
Cefuroxime (5) + + - - - - =MIC
Ceftazidime (30) + + - - - - =MIC
Cefotaxime (30) + + - - - - =MIC
Fosfomycin (200) - - - - - - NA
Polymyxin B (300 IU) +/- - - - + - =MIC
Gentamicin (10) - - - - - - NA
Chloramphenicol (30) - - - - - - NA
Doxycycline (30) - - - - - - NA
Erythromycin (15) - - - - - - NA
Rifampin (25) - - +/- - - - NA

aStx2 induction effects (zones of blue coloration, Figure 1): -, not detected, +/-, borderline induction, +, definite induction, >MIC, zone of induction within the zone of growth inhibition; =MIC, induction on the edge of the zone of inhibition; NA, not applicable; Amoxy/Clav, amoxycillin/clavulanic acid; Pip/Taz, piperacillin/tazobactam; Smx, sulphamethoxazole.
bO2, aerobic; mO2 microaerobic; AO2, anaerobic/incubation temperature °C.
cNote: The patterns indicated were detected in three separate experiments and recorded by two independent observers.
dThe quinolones tested are listed in the Materials and Methods section.

Main Article

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