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Volume 7, Number 3—June 2001
Dispatch

VEB-1-like Extended-Spectrum ß-Lactamases in Pseudomonas aeruginosa, Kuwait

Laurent Poirel*, Vincent O. Rotimi†, Eiman M. Mokaddas†, Amal Karim*, and Patrice Nordmann*Comments to Author 
Author affiliations: *Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre, France; †Kuwait University, Kuwait

Main Article

Figure

Comparison of the amino acid sequence of VEB-1, VEB-1a, and VEB-1b beta-lactamases from Pseudomonas aeruginosa JES from Thailand and KU-1 and KU-2 from Kuwait. Dashed lines indicate identical amino acids. Arrow indicates position of the putative cleavage site of the peptide leader. Numbering is according to Ambler designation (12).

Figure. . Comparison of the amino acid sequence of VEB-1, VEB-1a, and VEB-1b beta-lactamases from Pseudomonas aeruginosa JES from Thailand and KU-1 and KU-2 from Kuwait. Dashed lines indicate identical amino acids. Arrow indicates position of the putative cleavage site of the peptide leader. Numbering is according to Ambler designation (12).

Main Article

References
  1. Chen  HY, Yuan  M, Livermore  DM. Mechanisms of resistance to ß-lactam antibiotics amongst Pseudomonas aeruginosa isolates collected in the UK in 1993. J Med Microbiol. 1995;43:300–9. DOIPubMedGoogle Scholar
  2. Nordmann  P, Guibert  M. Extended-spectrum ß-lactamases in Pseudomonas aeruginosa. J Antimicrob Chemother. 1998;42:128–31. DOIPubMedGoogle Scholar
  3. Nordmann  P, Naas  T. Sequence analysis of PER-1 extended-spectrum ß-lactamase from Pseudomonas aeruginosa and comparison with class A ß-lactamases. Antimicrob Agents Chemother. 1994;38:104–14.PubMedGoogle Scholar
  4. Vahaboglu  H, Ozturk  R, Aygun  G, Coskunkan  F, Yaman  A, Kaygusuz  A, Widespread detection of PER-type extended-spectrum ß-lactamases among nosocomial Acinetobacter and Pseudomonas aeruginosa isolates in Turkey: a nationwide multicenter study. Antimicrob Agents Chemother. 1997;41:2265–9.PubMedGoogle Scholar
  5. Poirel  L, Naas  T, Guibert  M, Chaibi  EB, Labia  R, Nordmann  P. Molecular and biochemical characterization of VEB-1, a novel class A extended-spectrum ß-lactamase encoded by an Escherichia coli integron gene. Antimicrob Agents Chemother. 1999;43:573–81.PubMedGoogle Scholar
  6. Naas  T, Poirel  L, Karim  A, Nordmann  P. Molecular characterization of In50, a class 1 integron encoding the gene for the extended-spectrum ß-lactamase VEB-1 in Pseudomonas aeruginosa. FEMS Microbiol Lett. 1999;176:411–9.PubMedGoogle Scholar
  7. Tribuddharat  C, Fennewald  M. Integron-mediated rifampin resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1999;43:960–2.PubMedGoogle Scholar
  8. Poirel  L, Naas  T, Nicolas  D, Collet  L, Bellais  S, Cavallo  JD, Characterization of VIM-2, a carbapenem-hydrolyzing metallo-ß-lactamase and its plasmid- and integron-borne gene from a Pseudomonas aeruginosa clinical isolate in France. Antimicrob Agents Chemother. 2000;44:891–7. DOIPubMedGoogle Scholar
  9. Mercier  J, Lévesque  RC. Cloning of SHV-2, OHIO-1 and OXA-6 ß-lactamase and cloning and sequencing of SHV-1 ß-lactamase. Antimicrob Agents Chemother. 1990;34:1577–83.PubMedGoogle Scholar
  10. Poirel  L, Guibert  M, Bellais  S, Naas  T, Nordmann  P. Integron-and carbenicillinase-mediated reduced susceptibility to amoxicillin-clavulanic-acid in isolates of multidrug-resistant Salmonella enterica serotype typhimurium DT104 from French patients. Antimicrob Agents Chemother. 1999;43:1098–2004.PubMedGoogle Scholar
  11. Poirel  L, Le Thomas  I, Naas  T, Karim  A, Nordmann  P. Biochemical-sequence analyses of GES-1, a novel class A extended-spectrum ß-lactamase, and the class 1 integron In52 from Klebsiella pneumoniae. Antimicrob Agents Chemother. 2000;44:622–32. DOIPubMedGoogle Scholar
  12. Ambler  RP. The structure of ß-lactamases. Philos Trans R Soc Lond B Biol Sci. 1980;289:321–31. DOIPubMedGoogle Scholar
  13. Fluit  AC, Schmitz  FJ. Class 1 integrons, gene cassettes, mobility, and epidemiology. Eur J Clin Microbiol Infect Dis. 1999;18:761–70. DOIPubMedGoogle Scholar
  14. Lévesque  C, Piché  L, Larose  C, Roy  PH. PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob Agents Chemother. 1995;39:185–91.PubMedGoogle Scholar
  15. Stokes  HW, O'Gorman  DB, Recchia  GD, Parsekhian  M, Hall  RM. Structure and function of 59-base element recombination sites associated with mobile gene cassettes. Mol Microbiol. 1997;26:731–45. DOIPubMedGoogle Scholar
  16. Tosini  F, Visca  P, Luzzi  I, Dionisi  AM, Pezella  C, Petrucca  A, Class 1 integron-borne multiple-antibiotic resistance carried by IncFI and IncL/M plasmids in Salmonella enterica serotype typhimurium. Antimicrob Agents Chemother. 1998;42:3053–8.PubMedGoogle Scholar
  17. Hall  RM, Brown  HJ, Brookes  DE, Stokes  HW. Integrons found in different locations have identical 5' ends but variable 3' ends. J Bacteriol. 1994;176:6286–94.PubMedGoogle Scholar
  18. Williams  JG, Kubelik  AR, Livak  KJ, Rafalski  JA, Tingey  SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990;18:6531–5. DOIPubMedGoogle Scholar
  19. Jamal  WY, El Din  K, Rotimi  VO, Chugh  TD. An analysis of hospital-acquired bacteraemia in intensive care unit patients in a university hospital in Kuwait. J Hosp Infect. 1999;43:49–56. DOIPubMedGoogle Scholar
  20. Mimoz  O, Elhelali  N, Léotard  S, Jacolot  A, Laurent  F, Samii  K, Treatment of experimental pneumonia in rats caused by a PER-1 extended-spectrum ß-lactamase-producing strain of Pseudomonas aeruginosa. J Antimicrob Chemother. 1999;44:91–7. DOIPubMedGoogle Scholar

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