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Volume 7, Number 4—August 2001
THEME ISSUE
West Nile Virus
West Nile Virus

The Relationships between West Nile and Kunjin Viruses

Jacqueline H. Scherret*, Michael Poidinger†, John S. Mackenzie*, Annette K. Broom‡, Vincent Deubel§, W. Ian Lipkin¶, Thomas Briese¶, Ernest A. Gould#, and Roy A. Hall*Comments to Author 
Author affiliations: *University of Queensland, Brisbane, Queensland, Australia; †Bioinformatics Inc., Eveleigh, New South Wales, Australia; ‡University of Western Australia, Nedlands, Western Australia, Australia; §Institut Pasteur, Paris, France; ¶University of California, Irvine, California, USA; #Institute of Virology and Environmental Microbiology, Oxford, England

Main Article

Table 4

Binding patterns of anti-KUN and anti-WN monoclonal antibodies to virus isolates in enzyme-linked immunosorbent assay (ELISA)a

Monoclonal antibodies (MAb)
Virus 10A1 546 2B2 2B4 3.91D 3.67G 3.1112G
KUNb +c - + + + + +
KUN MP502-66 - - + + + + +
WNd - + + + + + +
WN Sarafend - - + + - - +
KOU - - + + + + -

aInfected C6/36 cell monolayers in 96-well plates were fixed with acetone and used as the antigen in the ELISA.
bAll Australian KUN isolates exhibited identical MAb binding patterns.
cA result was considered positive if consecutive twofold dilutions of MAb produced an OD >0.25 and at least twice that shown on uninfected cells.
dAll West Nile isolates except Sarafend produced identical MAb binding patterns.

Main Article

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