Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 7, Number 4—August 2001
THEME ISSUE
West Nile Virus
West Nile Virus

Rapid Determination of HLA B*07 Ligands from the West Nile Virus NY99 Genome

Anne S. De Groot*†Comments to Author , Caitlin Saint-Aubin*, Andrew Bosma*, Hakima Sbai*, James Rayner†, and William Martin†
Author affiliations: *Brown University, Providence, Rhode Island, USA; †EpiVax, Inc., Providence, Rhode Island, USA

Main Article

Table 1

Scoring overlapping peptides by the EpiMatrix motif HLA B*07 EpiMatrix analysis of West Nile virus protein NS-1

AA start Peptide no. (B*07 rank) Sequence EBPa
1123 WNB7 3119 GMEIRPQRHD 0.04
1124 WNB7 2818 MEIRPQRHDE 0.08
1125 WNB7 0591 EIRPQRHDEK 1.12
1126 WNB7 2660 IRPQRHDEKT 0.10
1127 WNB7 0019 RPQRHDEKTL 22.49
1128 WNB7 2661 PQRHDEKTLV 0.10

aEBP = estimated binding potential, which is the value that EpiMatrix uses to describe the probability that the peptide will bind to B*07 in vitro and in vivo. In this example, six overlapping peptides in the region of the WNV sequence coding for the NS-1 protein (WNV genome AA 1123 to 1128) are shown. WNVB7 0019 received the best EpiMatrix score (22.49) and was therefore selected for in vitro studies.

Main Article

Page created: April 27, 2012
Page updated: April 27, 2012
Page reviewed: April 27, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external