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Volume 7, Number 4—August 2001
THEME ISSUE
West Nile Virus
West Nile Virus

Detection of North American West Nile Virus in Animal Tissue by a Reverse Transcription-Nested Polymerase Chain Reaction Assay

Donna J. JohnsonComments to Author , Eileen N. Ostlund, Douglas D. Pedersen, and Beverly J. Schmitt
Author affiliations: Animal and Plant Health Inspection Service, U. S. Department of Agriculture, Ames, Iowa, USA

Main Article

Figure 1

Visualization of reverse transcription-nested polymerase chain reaction product. West Nile virus-positive samples are indicated by a 248-bp band. Lane 1: positive crow brain, NY, 1999. Lane 2: positive crow kidney, NY, 1999. Lane 3: positive Sandhill Crane brain, CT, 1999. Lane 4: negative crow brain. Lane 5-6: positive crow brains, NY, 2000. Lane 7: normal control. Lane 8-10: positive horse brains, NY, 1999. Lane 11: negative horse brain. Lane 12: positive horse brain, RI, 2000. Lane 13: positi

Figure 1. . Visualization of reverse transcription-nested polymerase chain reaction product. West Nile virus-positive samples are indicated by a 248-bp band. Lane 1: positive crow brain, NY, 1999. Lane 2: positive crow kidney, NY, 1999. Lane 3: positive Sandhill Crane brain, CT, 1999. Lane 4: negative crow brain. Lane 5-6: positive crow brains, NY, 2000. Lane 7: normal control. Lane 8-10: positive horse brains, NY, 1999. Lane 11: negative horse brain. Lane 12: positive horse brain, RI, 2000. Lane 13: positive horse brain, NJ, 2000. Lane 14: positive horse brain, MA, 2000. Lane 15: positive horse brain, CT, 2000. Lane 16: normal control. Lane 17: NY99 West Nile virus control. L: 100-bp DNA ladder. Band artifacts in lanes 1, 3, 5, and 6 are due to large amount of virus present in those samples.

Main Article

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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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