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Volume 7, Number 5—October 2001
Research

First Isolation of La Crosse Virus from Naturally Infected Aedes albopictus

Reid R. Gerhardt*, Kristy L. Gottfried†Comments to Author , Charles S. Apperson‡, Brent S. Davis†, Paul C. Erwin§, A. Brent Smith*, Nicholas A. Panella†, Eugene E. Powell‡, and Roger S. Nasci†
Author affiliations: *University of Tennessee, Knoxville, Tennessee, USA; †Centers for Disease Control and Prevention, Fort Collins, Colorado, USA; ‡North Carolina State University, Raleigh, North Carolina, USA; §State of Tennessee Department of Health, East Tennessee Region, Knoxville, Tennessee, USA

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Figure 2

Species-specific gel electrophoresis of La Crosse virus-positive mosquito pools from Tennessee and North Carolina and Aedes albopictus pools with varying degrees of Ochlerotatus triseriatus contamination. Lane 1, 100-bp ladder; lane 2, Oc. triseriatus pool with Oc. triseriatus primers; lane 3, Oc. triseriatus pool with Aedes albopictus primers; lane 4, Ae. albopictus pool with Ae. albopictus primers; lane 5, Ae. albopictus pool with Oc. triseriatus primers; lane 6, Ae. albopictus pool contaminat

Figure 2. . Species-specific gel electrophoresis of La Crosse virus-positive mosquito pools from Tennessee and North Carolina and Aedes albopictus pools with varying degrees of Ochlerotatus triseriatus contamination. Lane 1, 100-bp ladder; lane 2, Oc. triseriatus pool with Oc. triseriatus primers; lane 3, Oc. triseriatus pool with Aedes albopictus primers; lane 4, Ae. albopictus pool with Ae. albopictus primers; lane 5, Ae. albopictus pool with Oc. triseriatus primers; lane 6, Ae. albopictus pool contaminated by 1 Oc. triseriatus individual, with Ae. albopictus primers; lane 7, Ae. albopictus pool contaminated by 1 Oc. triseriatus individual, with Oc. triseriatus primers; lane 8, TN00-2266 Ae. albopictus pool with Ae. albopictus primers; lane 9, TN00-2266 Ae. albopictus pool with Oc. triseriatus primers; lane 10, NC00-1547 Ae. albopictus pool with Ae. albopictus primers; lane 11, NC00-1547 Ae. albopictus pool with Oc. triseriatus primers; lane 12, Ae. albopictus pool contaminated by 1 Oc. triseriatus head, with Oc. triseriatus primers; lane 13, Ae. albopictus pool contaminated by 1 Oc. triseriatus thorax, with Oc. triseriatus primers; lane 14, Ae. albopictus pool contaminated by 1 Oc. triseriatus abdomen, with Oc. triseriatus primers; lane 15, Ae. albopictus pool contaminated by 3 Oc. triseriatus legs, with Oc. triseriatus primers; and lane 16, Ae. albopictus pool contaminated by 6 Oc. triseriatus legs, with Oc. triseriatus primers.

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Page updated: April 26, 2012
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