Volume 8, Number 10—October 2002
THEME ISSUE
Bioterrorism-related Anthrax
Bioterrorism-related Anthrax
Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis
Table 1
Origin, designations, and results of cell wall and capsule direct fluorescent-antibody assays for 230 Bacillus anthracis isolatesa
Origin | No. of isolates | Temporal range and geographic origin | MLVA genotypes representedb | CW-DFA (% positive) | CAP-DFA (% positive) |
---|---|---|---|---|---|
Human isolates | 31 | 1943–1997; Africa, Asia, Australia, Europe, North America | 3,4,22,23,28,32,34, 35,36,37,41,43,44, 45,50,66,68 | 31 (100) | 31 (100) |
Animal isolates | 29 | 1939–1997; Africa, Asia, Australia, Europe, North and South America | 3,10,20,26,29,30,35,38,40,45,48,49,51, 55,57,78,80,81,84, 85,87, 89 | 29 (100) | 29 (100) |
Environmental isolates (e.g., soil, burial sites, wool, tannery, mill) | 16 | 1950–1993; Africa, Asia, Europe, and North America | 13,14,21,24,47,62, 69,73,77,79,82 | 15 (94) | 16 (100) |
pX01 plasmid cured | 4 | 1950–1974; North America | 3 (75) | 4 (100) | |
pX02 plasmid cured | 1 | Africa | 1 (100) | 0 (0) | |
2001 anthrax outbreak | 149 | October 2001; United States | 62 | 149 (100) | 149 (100) |
Total | 230 | 228 (99) | 229 (99.6) |
aDFA, direct fluorescent antibody assay; CW, cell wall; CAP, capsule; MLVA, multiple-locus variable-number tandem repeat analysis.
bKeim P, et al. J Bacteriol 2000;182:2928–36 (19).
References
- Pile J, Malone J, Eitzen E, Friedlander A. Anthrax as a potential biological warfare agent. Arch Intern Med. 1998;158:429–34. DOIPubMedGoogle Scholar
- Inglesby T, Henderson D, Bartlett J, Ascher M, Eitzen E, Friedlander A, Anthrax as a biological weapon. JAMA. 1999;281:1735–45. DOIPubMedGoogle Scholar
- Christopher G, Cieslak T, Pavlin A, Eitzen E. Biological warfare. A historical perspective. JAMA. 1997;278:412–7. DOIPubMedGoogle Scholar
- Kliemann W, Ruoff K. Bioterrorism: implications for the clinical microbiologist. Clin Microbiol Rev. 2001;14:364–81. DOIPubMedGoogle Scholar
- Khan A, Morse S, Lillibridge S. Public-health preparedness for biological terrorism in the USA. Lancet. 2000;356:1179–82. DOIPubMedGoogle Scholar
- Jernigan J, Stephens D, Ashford D, Omenaca C, Topiel M, Galbraith M, Bioterrorism-related inhalational anthrax: the first 10 cases reported in the United States. Emerg Infect Dis. 2001;7:933–48.PubMedGoogle Scholar
- Isenberg H. Clinical microbiology procedures handbook. vol.1 & 2. Washington: American Society for Microbiology Press; 1992.
- Phillips A, Ezzell J. Identification of Bacillus anthracis by polyclonal antibodies against extracted vegetative cell antigens. J Appl Bacteriol. 1989;66:419–32.PubMedGoogle Scholar
- Franek J. Application of fluorescent antibodies for demonstrating B. anthracis in the organs of infected animals. J Hyg Epidemiol Microbiol Immunol. 1964;8:111–9.
- Helgason E, Okstad D, Caugant H, Johansen A, Fouet M, Mock M, Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis—one species on the basis of genetic evidence. Appl Environ Microbiol. 2000;66:2627–30. DOIPubMedGoogle Scholar
- Ezzell J, Abshire T. Immunological analysis of cell-associated antigens of Bacillus anthracis. Infect Immun. 1988;56:349–56.PubMedGoogle Scholar
- Ezzell J, Abshire T, Little S, Lidgerding B, Brown C. Identification of Bacillus anthracis by using monoclonal antibody to cell wall galactose-N-acetylglucosamine polysaccharide. J Clin Microbiol. 1990;28:223–31.PubMedGoogle Scholar
- Fouet A, Mesnage S, Tosi-Couture E, Gounon P, Mock M. Bacillus anthracis surface: capsule and S-layer. J Appl Microbiol. 1999;87:251–5. DOIPubMedGoogle Scholar
- Etienne-Toumelin I, Sirard J, Duflot E, Mock M, Fouet A. Characterization of the Bacillus anthracis S-layer: cloning and sequencing of the structural gene. J Bacteriol. 1995;177:614–20.PubMedGoogle Scholar
- Ezzell J, Welkos S. The capsule of Bacillus anthracis, a review. J Appl Microbiol. 1999;87:250. DOIPubMedGoogle Scholar
- Ezzell J, Abshire T. Encapsulation of Bacillus anthracis spores and spore identification. Proceedings of the International Workshop on Anthrax. Salisbury Medical Bulletin 1999:87:42.
- Mina B, Dym F, Kuepper F, Tso R, Arrastia C, Kaplounova I, Fatal inhalational anthrax with unknown source of exposure in a 61-year-old woman in New York City. JAMA. 2002;287:858–62. DOIPubMedGoogle Scholar
- Barakat L, Quentzel H, Jernigan J, Kirschke D, Griffith K, Spear S, Fatal inhalational anthrax in a 94-year-old Connecticut women. JAMA. 2002;287:863–8. DOIPubMedGoogle Scholar
- Keim P, Price L, Klevytska A, Smith K, Schupp J, Okinaka R, Multi-locus variable-number tandem repeat analysis reveals genetic relationships with Bacillus anthracis. J Bacteriol. 2000;182:2928–36. DOIPubMedGoogle Scholar
- Hoffmaster A, Meyer R, Bowen M, Marston C, Weyant R, Barnett G, Evaluation and validation of a real-timepolymerase chain reaction assay for rapid identification of Bacillus anthracis. Emerg Infect Dis. 2002;8:1178–82.PubMedGoogle Scholar