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Volume 8, Number 2—February 2002


Traditional and Molecular Techniques for the Study of Emerging Bacterial Diseases: One Laboratory’s Perspective

Pierre Houpikian and Didier RaoultComments to Author 
Author affiliations: Unité des Rickettsies, Faculté de Médecine de Marseille, Marseille, France

Main Article

Table 2

Key issues for isolating main emerging bacteria

Conditions for incubation
Group Axenic specific medium Living system (embryonated egg,
cell line) Low temperature
(<37°C) O2 and CO2 conditions Extended incubation
Alpha1 Proteo-
bacteriae Ehrlichia sp.
Rickettsia sp.
Chlamydia sp. ELB agent
(“Rickettsia felis”) (28°C) Ehrlichia sp.
Rickettsia sp.
Alpha2 Proteo-
bacteriae Afipia sp. Afipia sp.
Bartonella sp. Bartonella bacilliformis (28°C) Bartonella sp.
Spirochetae Borrelia sp. Treponema pallidum
Delta-Xi Proteo-
bacteriae Campylobacter sp. (microaerophilic)
Helicobacter sp. (microaerophilic) Helicobacter pylori
Gamma Proteo-
bacteriae Legionella sp. Legionella sp. Yersinia pestis
Mycobacteria Mycobacterium sp. Mycobacterium leprae Mycobacterium malmoense (microaerophilic) Mycobacterium sp.
Mycoplasmas Mycoplasma sp. Mycoplasma fermentans
Gram-positive bacteria Tropheryma whipplei Clostridium difficile
(anaerobic) Tropheryma whipplei

Main Article