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Volume 8, Number 2—February 2002

Epidemiology of Burkholderia cepacia Complex in Patients with Cystic Fibrosis, Canada

David P. Speert*, Deborah Henry*, Peter Vandamme†, Mary Corey‡, and Eshwar Mahenthiralingam*
Author affiliations: *University of British Columbia and Children’s and Women’s Health Centre of British Columbia, Vancouver, British Columbia, Canada; †University of Ghent, Ghent, Belgium; ‡The Hospital for Sick Children, Toronto, Ontario, Canada

Main Article


Phylogenetic analysis of the recA gene from the Burkholderia cepacia complex. The phylogenetic diversity of the B. cepacia complex observed after nucleotide sequence analysis of the recA gene is shown. Isolates recovered from Canadian CF patients that are representative of strains of currently indeterminate genomovar status (Table 2) appear in bold and lack species identification; all fall within the current B. cepacia complex. The tree was drawn as described (16). The recA sequence from Bordete

Figure. . Phylogenetic analysis of the recA gene from the Burkholderia cepacia complex. The phylogenetic diversity of the B. cepacia complex observed after nucleotide sequence analysis of the recA gene is shown. Isolates recovered from Canadian CF patients that are representative of strains of currently indeterminate genomovar status (Table 2) appear in bold and lack species identification; all fall within the current B. cepacia complex. The tree was drawn as described (16). The recA sequence from Bordetella pertussis was used as a root, and the genetic distance is indicated by the bar.

Main Article

  1. Govan  JRW, Deretic  V. Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol Rev. 1996;60:53974.PubMedGoogle Scholar
  2. Speert  DP, Bond  M, Woodman  RC, Curnutte  JT. Infection with Pseudomonas cepacia in chronic granulomatous disease: role of nonoxidative killing by neutrophils in host defense. J Infect Dis. 1994;170:152431.PubMedGoogle Scholar
  3. Isles  A, Maclusky  I, Corey  M, Gold  R, Prober  C, Fleming  P, Pseudomonas cepacia infection in cystic fibrosis: an emerging problem. J Pediatr. 1984;104:20610. DOIPubMedGoogle Scholar
  4. Govan  JR, Brown  PH, Maddison  J, Doherty  CJ, Nelson  JW, Dodd  M, Evidence for transmission of Pseudomonas cepacia by social contact in cystic fibrosis. Lancet. 1993;342:159. DOIPubMedGoogle Scholar
  5. Johnson  WM. Intercontinental spread of a highly transmissible clone of Pseudomonas cepacia proved by multilocus enzyme electrophoresis and ribotyping. Can J Infect Dis. 1994;5:868.
  6. LiPuma  JJ, Dasen  SE, Nielson  DW, Stern  RC, Stull  TL. Person-to-person transmission of Pseudomonas cepacia between patients with cystic fibrosis. Lancet. 1990;336:10946. DOIPubMedGoogle Scholar
  7. Medical/Scientific Advisory Committee—Canadian Cystic Fibrosis Foundation. Epidemiology of Pseudomonas cepacia in cystic fibrosis. Can J Infect Dis. 1993;4:1635.
  8. Pegues  CF, Pegues  DA, Ford  DS, Hibberd  PL, Carson  LA, Raine  CM, Burkholderia cepacia respiratory tract acquisition: epidemiology and molecular characterization of a large nosocomial outbreak. Epidemiol Infect. 1996;116:30917.PubMedGoogle Scholar
  9. Pegues  DA, Carson  LA, Tablan  OC, FitzSimmons  SC, Roman  SB, Miller  JM, Acquisition of Pseudomonas cepacia at summer camps for patients with cystic fibrosis. J Pediatr. 1994;124:694702. DOIPubMedGoogle Scholar
  10. Corey  M, Farewell  V. Determinants of mortality from cystic fibrosis in Canada, 1970-1989. Am J Epidemiol. 1996;143:100717.PubMedGoogle Scholar
  11. Vandamme  P, Holmes  B, Vancanneyt  M, Coenye  T, Hoste  B, Coopman  R, Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov. Int J Syst Bacteriol. 1997;47:1188200.PubMedGoogle Scholar
  12. LiPuma  JJ. Burkholderia cepacia: management issues and new insights. Clin Chest Med. 1998;19:47386. DOIPubMedGoogle Scholar
  13. Sun  L, Jiang  R, Steinbach  S, Holmes  A, Campanelli  C, Forstner  J, The emergence of a highly transmissible lineage of cbl+ Pseudomonas (Burkholderia) cepacia causing CF centre epidemics in North America and Britain. Nat Med. 1995;1:6616. DOIPubMedGoogle Scholar
  14. Mahenthiralingam  E, Simpson  DA, Speert  DP. Identification and characterization of a novel DNA marker associated with epidemic Burkholderia cepacia strains recovered from patients with cystic fibrosis. J Clin Microbiol. 1997;35:80816.PubMedGoogle Scholar
  15. Henry  DA, Mahenthiralingam  E, Vandamme  P, Coenye  T, Speert  DP. Phenotypic methods for determining genomovar status of the Burkholderia cepacia complex. J Clin Microbiol. 2001;39:10738. DOIPubMedGoogle Scholar
  16. Henry  DA, Campbell  ME, LiPuma  JJ, Speert  DP. Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium. J Clin Microbiol. 1997;35:6149.PubMedGoogle Scholar
  17. Mahenthiralingam  E, Bischof  J, Byrne  SK, Radomski  C, Davies  JE, Av-Gay, et al. DNA-based diagnostic approaches for the identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, Burkholderia cepacia genomovars I and III. J Clin Microbiol. 2000;38:316573.PubMedGoogle Scholar
  18. Coenye  T, Mahenthiralingam  E, Henry  D, LiPuma  JJ, Laevens  S, Gillis  M, Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. Int J Syst Evol Microbiol. 2001;51:148190.PubMedGoogle Scholar
  19. Whitby  PW, Pope  LC, Carter  KB, LiPuma  JJ, Stull  TL. Species-specific PCR as a tool for the identification of Burkholderia gladioli. J Clin Microbiol. 2000;38:2825.PubMedGoogle Scholar
  20. Mahenthiralingam  E, Campbell  ME, Henry  DA, Speert  DP. Epidemiology of Burkholderia cepacia infection in patients with cystic fibrosis: analysis by random amplified polymorphic DNA (RAPD) fingerprinting. J Clin Microbiol. 1996;34:291420.PubMedGoogle Scholar
  21. Mahenthiralingam  E, Campbell  ME, Foster  J, Lam  JS, Speert  DP. Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis. J Clin Microbiol. 1996;34:112935.PubMedGoogle Scholar
  22. Coenye  T, Falsen  E, Hoste  B, Ohlen  M, Goris  J, Govan  JRW, Description of Pandoraea gen. nov. with Pandoraea pulmonicola sp. nov., Pandoraea apista sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp. nov. and Pandoraea norimbergensis comb. nov. Int J Syst Evol Microbiol. 2000;50:88799.PubMedGoogle Scholar
  23. Coenye  T, Laevens  S, Willems  A, Ohlen  M, Hannant  W, Govan  JRW, Burkholderia fungorum sp. nov. and Burkholderia caledonica sp. nov., two new species isolated from the environment, animals and human clinical samples. Int J Syst Evol Microbiol. 2001;51:1099107.PubMedGoogle Scholar
  24. Mahenthiralingam  E, Vandamme  P, Campbell  ME, Henry  DA, Gravelle  AM, Wong  LTK, Infection with Burkholderia cepacia complex genomovars in patients with cystic fibrosis: virulent transmissible strains of genomovar III can replace B. multivorans. Clin Infect Dis. 2001;33:146975. DOIPubMedGoogle Scholar
  25. Burdge  DR, Noble  MA, Campbell  ME, Krell  VL, Speert  DP. Xanthomonas maltophilia misidentified as Pseudomonas cepacia in cultures of sputum from patients with cystic fibrosis: a diagnostic pitfall with major clinical implications. Clin Infect Dis. 1995;20:4458.PubMedGoogle Scholar
  26. McMenamin  JD, Zaccon  TM, Coenye  T, Vandamme  P, LiPuma  JJ. Misidentification of Burkholderia cepacia in U.S. cystic fibrosis treatment centers: an analysis of 1051 recent sputum isolates. Chest. 2000;117:16615. DOIPubMedGoogle Scholar
  27. Balandreau  J, Viallard  V, Cournoyer  B, Coenye  T, Laevens  S, Vandamme  P. Burkholderia cepacia genomovar III is a common plant-associated bacterium. Appl Environ Microbiol. 2001;67:9825.[REMOVED ADVANCE FIELD][REMOVED ADVANCE FIELD] DOIPubMedGoogle Scholar
  28. Fiore  A, Laevens  S, Bevivino  A, Dalmastri  C, Tabacchioni  S, Vandamme  P, Burkholderia cepacia complex: distribution of genomovars among isolates from the maize rhizosphere in Italy. Environ Microbiol. 2001;3:13743. DOIPubMedGoogle Scholar
  29. Whiteford  ML, Wilkinson  JD, McColl  JH, Conlon  FM, Michie  JR, Evans  TJ, Outcome of Burkholderia (Pseudomonas) cepacia colonisation in children with cystic fibrosis following a hospital outbreak. Thorax. 1995;50:11948. DOIPubMedGoogle Scholar
  30. Segonds  C, Heulin  T, Marty  N, Chabanon  G. Differentiation of Burkholderia species by PCR-restriction fragment length polymorphism analysis of the 16S rRNA gene and application to cystic fibrosis isolates. J Clin Microbiol. 1999;37:22018.PubMedGoogle Scholar
  31. Cheng  K, Smyth  RL, Govan  JRW, Doherty  C, Winstanley  C, Denning  N, Spread of B-lactam-resistant Pseudomonas aeruginosa in a cystic fibrosis clinic. Lancet. 1996;348:63942. DOIPubMedGoogle Scholar
  32. Jones  AM, Govan  JRW, Doherty  CJ, Dodd  ME, Isalska  BJ, Stanbridge  TN, Spread of multiresistant strain of Pseudomonas aeruginosa in an adult cystic fibrosis clinic. Lancet. 2001;358:5578. DOIPubMedGoogle Scholar
  33. Armstrong  DS, Nixon  G, Carlin  J, Carzino  R, Grimwood  K. Long-term outbreak of a transmissible virulent strain of Pseudomonas aeruginosa in a pediatric cystic fibrosis clinic. Pediatr Pulmonol. 2000;Suppl 20:393A.
  34. Snell  GI, de Hoyos  A, Krajden  M, Winton  T, Maurer  JR. Pseudomonas cepacia in lung transplant recipients with cystic fibrosis. Chest. 1993;103:46671. DOIPubMedGoogle Scholar
  35. Butler  SL, Doherty  CJ, Hughes  JE, Nelson  JW, Govan  JRW. Burkholderia cepacia and cystic fibrosis: do natural environments present a potential hazard? J Clin Microbiol. 1995;33:10014.PubMedGoogle Scholar
  36. Mortensen  JE, Fisher  MC, LiPuma  JJ. Recovery of Pseudomonas cepacia and other Pseudomonas species from the environment. Infect Control Hosp Epidemiol. 1995;16:302.PubMedGoogle Scholar
  37. Govan  JRW, Hughes  JE, Vandamme  P. Burkholderia cepacia: medical, taxonomic and ecological issues. J Med Microbiol. 1996;45:115.PubMedGoogle Scholar

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Page updated: April 18, 2012
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