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Volume 8, Number 5—May 2002

Risk to Human Health from a Plethora of Simian Immunodeficiency Viruses in Primate Bushmeat

Martine Peeters*Comments to Author , Valerie Courgnaud*, Bernadette Abela†, Philippe Auzel†‡, Xavier Pourrut*, Frederic Bibollet-Ruche§, Severin Loul†, Florian Liegeois*, Cristelle Butel*, Denis Koulagna¶, Eitel Mpoudi-Ngole†, George M. Shaw§, Beatrice H. Hahn§, and Eric Delaporte*
Author affiliations: *Institut de Recherche pour le Développement (IRD), Montpellier, France; †Projet Prevention du Sida au Cameroun (PRESICA), Yaounde, Cameroon; ‡Faculté Universitaire des Sciences Agronomiques de Gembloux, Gembloux, Belgium; §University of Alabama at Birmingham, Birmingham, Alabama, USA; ¶Ministry of Environment and Forestry, Yaounde, Cameroon;

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Table 3

Polymerase chain reaction (PCR) amplification of Simian immunodeficiency virus (SIV) sequences

Genus Species INNO-LIA posa
PCR pos/tested INNO-LIA ind
PCR pos/tested INNO-LIA neg
PCR pos/tested
Cercocebus agilis 0/6 0/8 0/13
torquatus 0/1
Lophocebus albigena 0/2 0/2 0/7
Cercopithecus cephus 2/25 0/7 0/56
mona ½ 0/2
neglectus 8/9 0/4
nictitans 3/21 1/1 0/61
pogonias 0/9 0/3 0/34
Chlorocebus tantalus 0/1 0/2
Miopithecus ogouensis 2/3 0/10
Erythrocebus patas 0/7
Colobus guereza 6/6 0/1 1/16
Mandrillus sphinx 4/5 0/1 0/4
Papio anubis 0/2 0/11
Total 26/91 1/23 1/228

aDNA was extracted from a subset of seropositive (pos), indeterminant (ind) and negative (neg) blood samples and subjected to nested PCR amplification by using HIV/SIV consensus pol primer pairs. In each column, the number of PCR-positive samples per total number of samples tested is indicated. The authenticity of all amplification products was confirmed by sequence analysis.

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