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Volume 9, Number 12—December 2003


Comparative Molecular and Microbiologic Diagnosis of Bacterial Endocarditis

Isabelle Podglajen*†, Fabienne Bellery*, Claire Poyart†, Philippe Coudol*, Annie Buu-Hoï*, Patrick Bruneval*, and Jean-Luc Mainardi*†Comments to Author 
Author affiliations: *Hôpital Européen Georges Pompidou, Paris, France; †INSERM E0004, Université Paris VI, Paris, France

Main Article


Comparison of results obtained from blood cultures with conventional methods of identification (CMI-BC) and from valves with sequence-based identification (SBI-V)

Positive blood culture (n = 30) Negative blood culture (n = 6)
PCR positive (n = 26)
PCR negative (n = 4)a
PCR positive (n = 5)
PCR negative
n = 1
Species identification
(n = 16)
Discordance (n = 10)


Streptococcus mutansS. sanguisS. gallolyticus (4)
Campylobacter fetusEscherichia colibPropionibacterium acnesStaphylococcus aureus (2)
Streptococcus oralis (2)cS. mitis (2)cS. pneumoniaec S. agalactiaeGemella spp.
Group C streptococci
S. mitis (2)
S. sanguisS. salivariusHaemophilus influenzaeH. aphrophilusStreptococcus spp. Lactobacillus crispatusAerococcus urinaeAbiotrophia adiacensS. sanguis (2)
S. oraliscS. oraliscH. aphrophilusH. paraphrophilusS. gordonii Staphylococcus aureusS. epidermidisS. capraeS. aureus +Strep. pneumonia formula image+ E. coli S. gallolyticus Bartonella quintana (3)
B. henselae

aIn the case of positive blood cultures with Staphylococcus aureus, Escherichia coli, and Streptococcus (Strep) pneumoniae, PCR was positive, but direct sequencing was not interpretable.
bSpecies identification based on sequence analysis of PCR-amplified rpoBint
cSpecies identification based on sequence analysis of PCR-amplified sodAint
dThree species were identified in the blood culture.

Main Article