Volume 9, Number 6—June 2003
Rift Valley Fever Virus Infection among French Troops in Chad
To the Editor: During the rainy season every year, outbreaks of self-limiting nonmalarious febrile syndromes have occurred in French military troops on duty in Chad. To determine the cause of these syndromes, the Tropical Medicine Institute of the French Army Medical Corps implemented an arbovirus surveillance program in Marseille.
During summer 2001, we collected samples from 50 soldiers who had a febrile illness. All blood spot samples tested negative by enzyme-linked immunosorbent assay (ELISA) for certain antigens (i.e., dengue virus, West Nile virus, Chikungunya virus, and Wesselsbron virus). However, after co-culture of 31 peripheral blood lymphocyte samples with C6/36 and Vero cell lines collected in NDjamena, Chad, in August to September 2001, two strains of Rift Valley fever virus (RVFV) were isolated and identified by using indirect immunofluorescence with a specific mouse ascitic fluid and by using reverse transcriptase-polymerase chain reaction (RT-PCR) and sequencing. In retrospective testing, we found that all serum specimens tested by ELISA for RVFV-specific immunoglobulin (Ig) M and IgG were negative. The second serum samples from the two case-patients with these strains, collected 2 months later, were strongly positive (IgM 1/200,000; IgG 1/5,000).
Rift Valley fever, a febrile disease that affects livestock and humans, is transmitted by mosquitoes and caused by a virus (genus: Phlebovirus, family: Bunyaviridae) that can persist in nature in contaminated eggs. The virus was first isolated in Kenya in 1930 (1) and is endemic in the region. In Chad, the disease was first reported in 1967 at the same time as in Cameroon (2); no strain was isolated at that time. Since 1977, RVFV infection resulted in 600 deaths in Egypt (3), 300 in Mauritania in 1987 (4), and 200 in Saudi Arabia and Yemen (5,6) in 2000 to 2001.
To characterize these RVFV strains, parts of the three genome segments (L, M, and S) were amplified by using RT-PCR and sequenced as described (7,8). The figure shows the phylogenic tree constructed from the sequence of the region coding for NSs in the S segment, by using the neighbor-joining method implemented in Clustal W (version 1.6; available from: URL: http://www-igbmc.u-strasbg.fr/BioInfo/ClustalW/clustalw.html). The two strains identified in Chad are quite similar. They are located within the East/Central lineage established previously (6,7), which contains the virus that circulated in Madagascar (1991), Kenya (1997–1998), and Yemen and Saudi Arabia (2000–2001) (9,10). Sequencing of the region in the M and L segments led to the same clustering (not shown), suggesting that this virus did not evolve by reassortment. Determining the origin of the virus is difficult, but its genetic properties suggest that this strain has a Kenyan origin. Before this isolation, no RVFV strains from Chad had been genetically characterized. This strain may be endemic in this region of Central Africa, or the RVFV strain circulating in the Eastern countries may have been transported outside of the territory (which was likely the case in Yemen and Saudi Arabia in 2000) (9,10). Of the two case-patients, one soldier did not leave NDjamena during his 3-month tour of duty, whereas the other had been in contact with livestock in a flooded area before onset of symptoms. Contamination may have occurred through infected animals or mosquitoes, although sheep living in the area did not show any sign of disease (i.e., spontaneous abortions, deaths). The two cases we describe were self-limiting; however, deaths from this illness have been reported in nonepidemic settings in Central African Republic (11). Our data emphasize that healthcare providers should systematically consider Rift Valley fever as a diagnosis for febrile syndromes in persons returning from Africa, even in nonepidemic settings (12).
- Daubney R, Hudson JR, Graham PC. Enzootic hepatitis of Rift Valley fever, an undescribed virus disease of sheep, cattle and man from East Africa. J Pathol Bacteriol. 1931;34:545–79. DOIGoogle Scholar
- Maurice Y. Premières constatations sérologiques sur l’incidence de la maladie de Wesselsbron et de la Fièvre de la Vallée du Rift chez les ovins et les ruminants sauvages du Tchad et du Cameroun. Rev Elev Méd Vét Pays Trop Revue d’Elevage et de Médecine Vétérinaire des Pays Tropicaux. 1967;20:395–405.
- Meegan JM, Hoogstraal H, Moussa MI. An epizootic of Rift Valley fever in Egypt in 1977. Vet Rec. 1979;105:124–5. DOIPubMedGoogle Scholar
- Jouan A, Le Guenno B, Digoutte JP, Philippe B, Riou O, Adam F. An RVF epidemic in southern Mauritania. Ann Inst Pasteur Virol. 1988;139:307–8. DOIPubMedGoogle Scholar
- Nasher AAW, Shiban AK, Eriyani MA, Aly Bourgy A, Al Kohlani AH, Benbrake M, Outbreak of Rift Valley fever, Yemen, August–October 2000. Wkly Epidemiol Rec. 2000;75:392–5.PubMedGoogle Scholar
- Arishi H, Ageel A, Abdu Rahman M, Al Hazmi A, Arishi AR, Ayoola B, Outbreak of Rift Valley fever, Saudi Arabia, August–November 2000. MMWR Morb Mortal Wkly Rep. 2000;49:982–5.PubMedGoogle Scholar
- Sall AA, de Zanotto PM, Sene OK, Zeller HG, Digoutte JP, Thiongane Y, Genetic reassortment of Rift Valley fever virus in nature. J Virol. 1999;73:8196–200.PubMedGoogle Scholar
- Sall AA, de Zanotto PM, Zeller HG, Digoutte JP, Thiongane Y, Bouloy M. Variability of the NSs protein among Rift Valley fever virus isolates. J Gen Virol. 1997;78:2853–8.PubMedGoogle Scholar
- Miller BR, Godsey MS, Crabtree MM, Savage HM, Al-Mazrao Y, Al-Jeffri M, Isolation and genetic characterization of Rift Valley fever virus from Aedes vexans arabiensis, Kindom of Saudi Arabia. Emerg Infect Dis. 2002;8:1492–4.PubMedGoogle Scholar
- Shoemaker T, Boulianne C, Vincent MJ, Pezzanile L, Al-Qahtani MM, Al-Mazrou Y, Genetic analysis of viruses associated with emergence of Rift Valley fever in Saudi Arabia and Yemen, 2000–01. Emerg Infect Dis. 2002;8:1415–20.PubMedGoogle Scholar
- Meunier DMY, Madelon MC, Lesbordes JL, Georges AJ. La fièvre de la Vallée du Rift et les phléboviroses en République Centrafricaine. Bull Soc Pathol Exot. 1988;81:49–57.
- Durand JP, Richecoeur L, Peyrefitte C, Boutin JP, Davoust B, Zeller H, La Fièvre de la Vallée du Rift: infections sporadiques de militaires français hors des zones d’épidémies actuellement connues. Med Trop. 2002;62:291–4.
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Table of Contents – Volume 9, Number 6—June 2003
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Please use the form below to submit correspondence to the authors or contact them at the following address:
Jean Paul Durand, Laboratoire Associé au Centre National de Référence des Arbovirus, Unité de Virologie, IMTSSA, BP 46, 13998 Marseille Armées, France; fax: 01 40 61 31 51