Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 9, Number 8—August 2003
Research

Molecular Analysis of Echovirus 13 Isolates and Aseptic Meningitis, Spain

Ana Avellón*Comments to Author , Inmaculada Casas*, Gloria Trallero*, Carmen Pérez†, Antonio Tenorio*, and Gustavo Palacios‡
Author affiliations: *Instituto de Salud Carlos III, Madrid, Spain; †Doctor Negrín Hospital, Las Palmas de Gran Canaria, Canary Island; ‡Columbia University, New York, New York, USA

Main Article

Figure 5

Mapping of the echovirus (EV) 13 VP1 amino acids. The three-dimensional structure is projected onto EV11 structure (GenBank accession no. 1H8TA) with VP1 amino acid numbering according to EV11. The sequenced fragment is shown in red. Previously reported neutralizing antigenic sites for poliovirus, CBV4, and SVDV are shown in yellow. The enterovirus canyon structure is found in a similar location to the canyon structure of poliovirus as reported by (40).

Figure 5. Mapping of the echovirus (EV) 13 VP1 amino acids. The three-dimensional structure is projected onto EV11 structure (GenBank accession no. 1H8TA) with VP1 amino acid numbering according to EV11. The sequenced fragment is shown in red. Previously reported neutralizing antigenic sites for poliovirus, CBV4, and SVDV are shown in yellow. The enterovirus canyon structure is found in a similar location to the canyon structure of poliovirus as reported by (40).

Main Article

References
  1. Melnick  JL. Enteroviruses: polioviruses, coxsackievirus, echoviruses and newer enteroviruses. In: Raven L, editor. Fields virology. Philadelphia: Lippincott-Raven; 1996. p. 655–712.
  2. Family Picornaviridae. In: Regenmortel M, editor. Virus taxonomy. Seventh report of the International Committee on Taxonomy on Viruses. San Diego: Academic Press; 2000.
  3. Poyry  T, Kinnunen  L, Hyypia  T, Brown  B, Horsnell  C, Hovi  T, Genetic and phylogenetic clustering of enteroviruses. J Gen Virol. 1996;77:1699717. DOIPubMedGoogle Scholar
  4. Kunkel  U, Schreier  E. Genetic variability within the VP1 coding region of echovirus type 30 isolates. Arch Virol. 2000;145:145564. DOIPubMedGoogle Scholar
  5. Kunkel  U, Schreier  E. Characterization of echovirus 25 (ECV 25) in the VP1/2A gene junction region. Brief report. Arch Virol. 1999;144:22538. DOIPubMedGoogle Scholar
  6. Mulders  MN, Salminen  M, Kalkkinen  N, Hovi  T. Molecular epidemiology of coxsackievirus B4 and disclosure of the correct VP1/2A(pro) cleavage site: evidence for high genomic diversity and long-term endemicity of distinct genotypes. J Gen Virol. 2000;81:80312.PubMedGoogle Scholar
  7. Oberste  MS, Maher  K, Kilpatrick  DR, Flemister  MR, Brown  BA, Pallansch  MA. Typing of human enteroviruses by partial sequencing of VP1. J Clin Microbiol. 1999;37:128893.PubMedGoogle Scholar
  8. Caro  V, Guillot  S, Delpeyroux  F, Crainic  R. Molecular strategy for “serotyping” of human enteroviruses. J Gen Virol. 2001;82:7991.PubMedGoogle Scholar
  9. Norder  H, Bjerregaard  L, Magnius  LO. Homotypic echoviruses share aminoterminal VP1 sequence homology applicable for typing. J Med Virol. 2001;63:3544. DOIPubMedGoogle Scholar
  10. Casas  I, Palacios  GF, Trallero  G, Cisterna  D, Freire  MC, Tenorio  A. Molecular characterization of human enteroviruses in clinical samples: comparison between VP2, VP1, and RNA polymerase regions using RT nested PCR assays and direct sequencing of products. J Med Virol. 2001;65:13848. DOIPubMedGoogle Scholar
  11. Palacios  G, Casas  I, Tenorio  A, Freire  C. Molecular identification of enterovirus by analyzing a partial VP1 genomic region with different methods. J Clin Microbiol. 2002;40:18292. DOIPubMedGoogle Scholar
  12. Palacios  G, Casas  I, Cisterna  D, Trallero  G, Tenorio  A, Freire  C. Molecular epidemiology of echovirus 30: temporal circulation and substitution of single lineages. J Virol. 2002;76:49409. DOIPubMedGoogle Scholar
  13. Minor  PD, Ferguson  M, Evans  DM, Almond  JW, Icenogle  JP. Antigenic structure of polioviruses of serotypes 1, 2 and 3. J Gen Virol. 1986;67:128391. DOIPubMedGoogle Scholar
  14. Borrego  B, Carra  E, Garcia-Ranea  JA, Brocchi  E. Characterization of neutralization sites on the circulating variant of swine vesicular disease virus (SVDV): a new site is shared by SVDV and the related coxsackie B5 virus. J Gen Virol. 2002;83:3544.PubMedGoogle Scholar
  15. Nijhar  SK, Mackay  DK, Brocchi  E, Ferris  NP, Kitching  RP, Knowles  NJ. Identification of neutralizing epitopes on a European strain of swine vesicular disease virus. J Gen Virol. 1999;80:27782.PubMedGoogle Scholar
  16. Rossmann  MG. The canyon hypothesis. Hiding the host cell receptor attachment site on a viral surface from immune surveillance. J Biol Chem. 1989;264:1458790.PubMedGoogle Scholar
  17. Savolainen  C, Hovi  T, Mulders  MN. Molecular epidemiology of echovirus 30 in Europe: succession of dominant sublineages within a single major genotype. Arch Virol. 2001;146:52137. DOIPubMedGoogle Scholar
  18. Oberste  MS, Maher  K, Kennett  ML, Campbell  JJ, Carpenter  MS, Schnurr  D, Molecular epidemiology and genetic diversity of echovirus type 30 (E30): genotypes correlate with temporal dynamics of E30 isolation. J Clin Microbiol. 1999;37:392833.PubMedGoogle Scholar
  19. Echovirus type 13—United States, 2001. MMWR Morb Mortal Wkly Rep. 2001;50:77780.PubMedGoogle Scholar
  20. Cunney  R. Enteroviral infections: a common cause of viral meningitis. Epi-Insight. 2001;2:23.
  21. Recent increases in incidence of echoviruses 13 and 30 around Europe. Eurosurveillance Weekly. 2002;14:7.
  22. Diedrich  S, Schreier  E. Aseptic meningitis in Germany associated with echovirus type 13. Biomed Center Infectious Diseases. 2001;1:14. DOIPubMedGoogle Scholar
  23. Viral meningitis associated with increase in echovirus type 13. Communicable Disease Report–Weekly 2000;10:277,280.
  24. Noah  N. Recent increases in incidence of echovirus 13 and 30 around Europe. Eurosurveillence Weekly 2002;Feb 14:7. Available from: URL: http://www.eurosurv.org/update/
  25. Trallero  G, Casas  I, Avellón  A, Pérez  C, Tenorio  A, de la Loma  A. First epidemic of aseptic meningitis due to echovirus type 13 among Spanish children. Epidemiol Infect. 2003;130:2516. DOIPubMedGoogle Scholar
  26. Keino  M, Kanno  M, Hirasawa  K, Watari  T, Mikawa  M, Saito  K, Isolation of echovirus type 13 from patients of aseptic meningitis. Jpn J Infect Dis. 2001;54:24950.PubMedGoogle Scholar
  27. McMinn  P. Echovirus meningitis in Western Australia. Communicable Diseases—Australia 2001; September.
  28. Trallero  G, Casas  I, Tenorio  A, Echevarria  JE, Castellanos  A, Lozano  A, Enteroviruses in Spain: virological and epidemiological studies over 10 years (1988–97). Epidemiol Infect. 2000;124:497506. DOIPubMedGoogle Scholar
  29. Casas  I, Powell  L, Klapper  PE, Cleator  GM. New method for the extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay. J Virol Methods. 1995;53:2536. DOIPubMedGoogle Scholar
  30. Oberste  MS, Maher  K, Flemister  MR, Marchetti  G, Kilpatrick  DR, Pallansch  MA. Comparison of classic and molecular approaches for the identification of untypeable enteroviruses. J Clin Microbiol. 2000;38:11704.PubMedGoogle Scholar
  31. Stuart  AD, McKee  TA, Williams  PA, Harley  C, Shen  S, Stuart  DI, Determination of the structure of a decay accelerating factor-binding clinical isolate of echovirus 11 allows mapping of mutants with altered receptor requirements for infection. J Virol. 2002;76:7694704. DOIPubMedGoogle Scholar
  32. Halim  S, Ramsingh  AI. A point mutation in VP1 of coxsackievirus B4 alters antigenicity. Virology. 2000;269:8694. DOIPubMedGoogle Scholar
  33. Mullins  A. Changes in circulating enterovirus serotypes in the United States, 2001. In International Conference on Emerging Infectious Diseases, Atlanta, Georgia, USA, March 22–24, 2002. Slide session available from: URL: http://www.cdc.gov/iceid/webcast/latebreakers1.htm
  34. Chua  BH, McMinn  PC, Lam  SK, Chua  KB. Comparison of the complete nucleotide sequences of echovirus 7 strain UMMC and the prototype (Wallace) strain demonstrates significant genetic drift over time. J Gen Virol. 2001;82:262939.PubMedGoogle Scholar
  35. Chu  PY, Lin  KH, Hwang  KP, Chou  LC, Wang  CF, Shih  SR, Molecular epidemiology of enterovirus 71 in Taiwan. Arch Virol. 2001;146:589600. DOIPubMedGoogle Scholar
  36. Handsher  R, Shulman  LM, Abramovitz  B, Silberstein  I, Neuman  M, Tepperberg-Oikawa  M, A new variant of echovirus 4 associated with a large outbreak of aseptic meningitis. J Clin Virol. 1999;13:2936. DOIPubMedGoogle Scholar
  37. Oprisan  G, Combiescu  M, Guillot  S, Caro  V, Combiescu  A, Delpeyroux  F, Natural genetic recombination between co-circulating heterotypic enteroviruses. J Gen Virol. 2002;83:2193200.PubMedGoogle Scholar
  38. Norder  H, Bjerregaard  L, Magnius  LO. Open reading frame sequence of an Asian enterovirus 73 strain reveals that the prototype from California is recombinant. J Gen Virol. 2002;83:17218.PubMedGoogle Scholar
  39. Isolation of echovirus 13 from meningitis cases—September 2001 Fukushima. Infectious Agent Surveillance Report 2001;22(12 December).
  40. He  Y, Bowman  VD, Mueller  S, Bator  CM, Bella  J, Peng  X, Interaction of the poliovirus receptor with poliovirus. Proc Natl Acad Sci U S A. 2000;97:7984. DOIPubMedGoogle Scholar
  41. Rossmann  MG, He  Y, Kuhn  RJ. Picornavirus-receptor interactions. Trends Microbiol. 2002;10:32431. DOIPubMedGoogle Scholar

Main Article

Page created: December 22, 2010
Page updated: December 22, 2010
Page reviewed: December 22, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external