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Volume 10, Number 2—February 2004
THEME ISSUE
2004 SARS Edition
Laboratory Study

Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Shannon L. Emery*, Dean D. Erdman*Comments to Author , Michael D. Bowen*, Bruce R. Newton*, Jonas M. Winchell*, Richard F. Meyer*, Suxiang Tong*, Byron T. Cook*, Brian P. Holloway*, Karen A. McCaustland*, Paul A. Rota*, Bettina Bankamp*, Luis E. Lowe*, Tom G. Ksiazek*, William J. Bellini*, and Larry J. Anderson*
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Main Article

Table 2

Reproducibility of real-time RT-PCR assaysa

RNA transcript copy numberb
SARS1
 7.5 x 101
 7.5 x 102
 7.5 x 103
 7.5 x 104
 7.5 x 105
 7.5 x 106
CV within assay (%)c
 2.53
 0.96
 0.49
 0.69
 1.66
 0.7
CV between assays (%)d
 2.39
 1.09
 0.82
 0.64
 2.1
 0.79
SARS2
 2.0 x 101
 2 x 102
 2 x 103
 2 x 104
 2 x 105
 2 x 106
CV within assay (%)
 1.27
 0.57
 0.46
 0.72
 0.84
 0.67
CV between assays (%)
 1.54
 1.18
 0.93
 1.47
 1.54
 1.32
SARS3
CV within assay (%)
 0.8
 0.55
 0.65
 0.5
 0.27
 1.25
CV between assays (%) 0.94 0.64 1.07 1.13 1.24 1.65

aRT-PCR, reverse transcription–polymerase chain reaction; CV, coefficient of variation.
bTen-fold dilutions of the polymerase and nucleocapsid RNA transcripts; copies per reaction; dilution series thawed on 3 different days and assays performed in triplicate for each dilution.
cDetermined from three replicates within each assay.
dDetermined from three independent assays performed on different days.

Main Article

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Page updated: January 27, 2011
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