Table 1

Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Shannon L. Emery*, Dean D. Erdman*

, Michael D. Bowen*, Bruce R. Newton*, Jonas M. Winchell*, Richard F. Meyer*, Suxiang Tong*, Byron T. Cook*, Brian P. Holloway*, Karen A. McCaustland*, Paul A. Rota*, Bettina Bankamp*, Luis E. Lowe*, Tom G. Ksiazek*, William J. Bellini*, and Larry J. Anderson*

Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Main Article

Primers and probes used for real-time RT-PCR assays^a

Assay ID	Primer/ probe	Sequence (5′>3′)	Genomic region	Location^b
Primary diagnostic assay
SARS1	F	CAT GTG TGG CGG CTC ACT ATA T	RNA polymerase	15370-15392
	R	GAC ACT ATT AGC ATA AGC AGT TGT AGC A		15422-15449
	P	TTA AAC CAG GTG GAA CAT CAT CCG GTG		15395-15420
SARS2	F	GGA GCC TTG AAT ACA CCC AAA G	Nucleocapsid	28531-28552
	R	GCA CGG TGG CAG CAT TG		28581-28597
	P	CCA CAT TGG CAC CCG CAA TCC		28559-28574
SARS3	F	CAA ACA TTG GCC GCA AAT T	Nucleocapsid	29016-29034
	R	CAA TGC GTG ACA TTC CAA AGA		29063-29083
	P	CAC AAT TTG CTC CAA GTG CCT CTG CA		29036-29061
To confirm positive results
N3	F	GAA GTA CCA TCT GGG GCT GAG	Nucleocapsid	28432-28452
	R	CCG AAG AGC TAC CCG ACG		28383-28400
	P	CTC TTT CAT TTT GCC GTC ACC ACC AC		28406-28431
3′NTR	F	AGC TCT CCC TAG CAT TAT TCA CTG	3′ nontranslated region	29619-29642
	R	CAC CAC ATT TTC ATC GAG GC		29576-29595
	P	TAC CCT CGA TCG TAC TCC GCG T		29597-29618
M	F	TGT AGG CAC TGA TTC AGG TTT TG	Membrane protein	26951-26973
	R	CGG CGT GGT CTG TAT TTA ATT TA		27005-27027
	P	CTG CAT ACA ACC GCT ACC GTA TTG GAA		26974-27000

^aRT-PCR, reverse transcription–polymerase chain reaction; F, forward primer; R, reverse primer; P, probe.
^bLocation based on the severe acute respiratory syndrome–associated coronavirus, Urbani strain (GenBank accession number AY278741).

Main Article

Page created: January 27, 2011

Page updated: January 27, 2011

Page reviewed: January 27, 2011

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Volume 10, Number 2—February 2004
THEME ISSUE
2004 SARS Edition

Laboratory Study

Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Table 1

Volume 10, Number 2—February 2004 THEME ISSUE 2004 SARS Edition

Laboratory Study

Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Table 1

Volume 10, Number 2—February 2004
THEME ISSUE
2004 SARS Edition