Volume 10, Number 2—February 2004
THEME ISSUE
2004 SARS Edition
Laboratory Study
Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus
Shannon L. Emery*, Dean D. Erdman*
, Michael D. Bowen*, Bruce R. Newton*, Jonas M. Winchell*, Richard F. Meyer*, Suxiang Tong*, Byron T. Cook*, Brian P. Holloway*, Karen A. McCaustland*, Paul A. Rota*, Bettina Bankamp*, Luis E. Lowe*, Tom G. Ksiazek*, William J. Bellini*, and Larry J. Anderson*
, Michael D. Bowen*, Bruce R. Newton*, Jonas M. Winchell*, Richard F. Meyer*, Suxiang Tong*, Byron T. Cook*, Brian P. Holloway*, Karen A. McCaustland*, Paul A. Rota*, Bettina Bankamp*, Luis E. Lowe*, Tom G. Ksiazek*, William J. Bellini*, and Larry J. Anderson*
Table 4
Comparison of real-time RT-PCR assays with culture and conventional RT-PCRa
| SARS-CoV dilutionb | Conventional RT-PCR | Real-time RT-PCR |
||
|---|---|---|---|---|
| SARS1 | SARS2 | SARS3 | ||
| 10–4 |
3/3c |
3/3 |
3/3 |
3/3 |
| 10–5 |
3/3 |
3/3 |
3/3 |
3/3 |
| 10–6 |
3/3 |
3/3 |
3/3 |
3/3 |
| 10–7 |
3/3 |
3/3 |
3/3 |
3/3 |
| 10–8 |
0/3 |
3/3 |
3/3 |
3/3 |
| 10–9 |
0/3 |
0/3 |
1/3 |
0/3 |
| 10–10 | 0/3 | 0/3 | 0/3 | 0/3 |
aRT-PCR, reverse transcription–polymerase chain reaction; SARS-CoV, severe acute respiratory syndrome–associated coronavirus.
bSerial 10-fold dilution of SARS-CoV stock culture containing 1 x 107 PFUs/mL.
cNumber of positive results divided by the number of replicates tested.
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