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Volume 10, Number 2—February 2004
THEME ISSUE
2004 SARS Edition
Laboratory Study

Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Shannon L. Emery*, Dean D. Erdman*Comments to Author , Michael D. Bowen*, Bruce R. Newton*, Jonas M. Winchell*, Richard F. Meyer*, Suxiang Tong*, Byron T. Cook*, Brian P. Holloway*, Karen A. McCaustland*, Paul A. Rota*, Bettina Bankamp*, Luis E. Lowe*, Tom G. Ksiazek*, William J. Bellini*, and Larry J. Anderson*
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Main Article

Table 5

Results of real-time RT-PCR assay with specimens from patients with laboratory-confirmed SARS-CoV infectiona

Case ID Location Specimen ID Specimen Serology Vero E6
culture Conventional
RT-PCRb Real-time RT-PCR CTc values
SARS1 SARS2 SARS3 RNase P
05078
 Toronto
 2003756523
 Lung, RM
 N/A
 –
 +
 24.2
 21.6
 23
 23.9
2003756525
 Lung, RU
–
 +
 24.9
 21.5
 23
 23.7
05077
 Thailand
 2003756502
 Throat swab
 +
 +
 +
 37.5
 36.2
 39.8
 29.3
05000
 Hong Kong
 2003757035
 Lung, RU
 +
 –
 +
 26.7
 22.6
 24.1
 24.7
2003757036
 Lung, LU
–
 +
 27.2
 24.9
 26.5
 26
2003757037
 Lung, RM
–
 +
 34.9
 37.5
 31.9
 27.4
2003757038
 Lung, LL
–
 +
 29.6
 27
 28.6
 24.5
00220
 Utah, USA
 2003757508
 Sputum
 +
 +
 +
 24.7
 23
 24.8
 30.6
05001
 Vietnam
 2003757190
 Throat wash
 +
 +
 +
 23.7
 22.4
 24.1
 30.1
05008
 Vietnam
 2003757229
 Throat wash
 +
 –
 +
 35.5
 35.5
 36.7
 30
05010
 Vietnam
 2003757239
 Throat wash
 +
 –
 +
 31.1
 29.3
 31.5
 34.2
05013
 Vietnam
 2003757251
 Throat wash
 +
 –
 +
 29.5
 28.4
 30.3
 28.8
05017
 Vietnam
 2003757268
 Throat wash
 +
 +
 +
 26
 24.7
 26.4
 27.9
05316 Vietnam 2003759760 Throat swab N/A + N/A 25 25.3 28.2 28

aRT-PCR, reverse transcription–polymerase chain reaction; SARS-CoV, severe acute respiratory syndrome–associated coronavirus; CT, threshold cycle number; RM, right middle; RU, right upper; LU, left upper; LL, left lower; N/A, not applicable.
bRef. 5.
cValues shown mean of duplicate values.

Main Article

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Main Article

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Page updated: January 27, 2011
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