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Volume 11, Number 2—February 2005
Research

Spotted Fever Group and Typhus Group Rickettsioses in Humans, South Korea

Yeon-Joo Choi*1, Won-Jong Jang1*, Jong-Hyun Kim*, Ji-Sun Ryu*, Seung-Hyun Lee*, Kyung-Hee Park*, Hyung-Suk Paik†, Young-Sang Koh‡, Myung-Sik Choi§, and Ik-Sang Kim§Comments to Author 

Author affiliations: *Konkuk University, Choongbuk, Republic of Korea; †Pusan National University, Pusan, Republic of Korea; ‡Cheju National University College of Medicine, Jeju, Republic of Korea; §Seoul National University College of Medicine and Institute of Endemic Disease, Seoul, Republic of Korea

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Figure 3

Restriction fragment length polymorphism (RFLP) analysis of H2-products amplified with multiplex-nested primer set from seropositive sera. Ethidium bromide–stained polyacrylamide gels of AluI restriction endonuclease digestion of ≈230-bp rickettsial DNA amplified by using the nested primer H set WJ77/80 in the primary reactions and WJ79/83/78 in the nested reactions. Lanes: M, size marker DNA (25-bp DNA ladder); 1, H3-2; 2, H7-2; 3, H8-2; 4, H13-2; 5, H14-2; 6, H15-2; 7, H18-2; 8, H19; P, R. pro

Figure 3. Restriction fragment length polymorphism (RFLP) analysis of H2-products amplified with multiplex-nested primer set from seropositive sera. Ethidium bromide–stained polyacrylamide gels of AluI restriction endonuclease digestion of ≈230-bp rickettsial DNA amplified by using the nested primer H set WJ77/80 in the primary reactions and WJ79/83/78 in the nested reactions. Lanes: M, size marker DNA (25-bp DNA ladder); 1, H3-2; 2, H7-2; 3, H8-2; 4, H13-2; 5, H14-2; 6, H15-2; 7, H18-2; 8, H19; P, R. prowazekii; T, R. typhi. P and T; predicted fragments after digestion. The number on the left indicates the molecular size (in base pairs) of restriction fragments.

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1Y.-J. Choi and W.-J. Jang contributed equally to this work.

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