Volume 13, Number 7—July 2007
Dispatch
Rocky Mountain Spotted Fever, Colombia
Marylin Hidalgo*, Leonora Orejuela†, Patricia Fuya*, Pilar Carrillo‡, Jorge Hernandez§, Edgar Parra*, Colette Keng¶, Melissa Small¶, Juan P. Olano¶, Donald H. Bouyer¶, Elizabeth Castaneda*, David Walker¶, and Gustavo Valbuena†¶
Table 1
Rocky Mountain spotted fever patients and findings, Colombia, 2003–2004
| Patient | Confirmatory methods | Adult ticks collected in area where patients lived | |||||
|---|---|---|---|---|---|---|---|
| PCR | Sequence | IHA* | Animal inoculation | Culture | |||
| 1 | Genes | Primers | Homology with Rickettsia rickettsii | Positive with rabbit anti–spotted fever group rickettsial antibody | Not done | Not done | 15 Amblyomma cajennense, 184 Rhipicephalus sanguineus, 7 Anocentor nitens, and 8 Amblyomma spp. |
| 17-kDa | 17kD1/2 | 100% (Sheila Smith) | |||||
| 2 | Genes | Primers | Homology with
R. rickettsii | Positive with rabbit anti–spotted fever group rickettsial antibody | 24 h and 48 h after fever onset, 2 guinea pigs were euthanized for culture and PCR analysis of spleens | Vero cells with cytopathic changes after 1 week | 36 A. cajennense, 13 R. sanguineus, and 38 Boophilus microplus |
| gltA | CS78/323 | 99% (Bitterroot and others) | |||||
| gltA | CS5/6 | 94% (Bitterroot) | |||||
| OmpA | 190.70/701 | 98% (strain 1995HO2 and others) | |||||
| OmpB | rOmpB.20-2788 | 98% (GenBank accession no. X16353.1) | |||||
*Immunohistochemical analysis.
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