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Volume 15, Number 3—March 2009
Dispatch

Detection of Newly Described Astrovirus MLB1 in Stool Samples from Children

Stacy R. Finkbeiner, Binh-Minh Le, Lori R. Holtz, Gregory A. Storch, and David WangComments to Author 
Author affiliations: Washington University School of Medicine, St. Louis, Missouri, USA

Main Article

Figure 2

Phylogenetic analysis of astrovirus MLB1 (AstV-MLB1) isolates. A region of the serine protease (A) and the capsid (B) of each virus detected by the AstV-MLB1–specific primers was amplified and sequenced. Multiple sequence alignments were then generated with these sequences and the corresponding regions of known astroviruses using ClustalX (www.clustal.org). PAUP* (Sinauer Associates, Sunderland, MA, USA) was used to generate phylogenetic trees; bootstrap values (>700) from 1,000 replicates ar

Figure 2. Phylogenetic analysis of astrovirus MLB1 (AstV-MLB1) isolates. A region of the serine protease (A) and the capsid (B) of each virus detected by the AstV-MLB1–specific primers was amplified and sequenced. Multiple sequence alignments were then generated with these sequences and the corresponding regions of known astroviruses using ClustalX (www.clustal.org). PAUP* (Sinauer Associates, Sunderland, MA, USA) was used to generate phylogenetic trees; bootstrap values (>700) from 1,000 replicates are shown. The previously identified AstV-MLB1 isolate (9,10) and the isolates from this study are shown in boldface. Scale bars indicate number of amino acid substitutions per site.

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References
  1. Chu  DK, Poon  LL, Guan  Y, Peiris  JS. Novel astroviruses in insectivorous bats. J Virol. 2008;82:9107–14. DOIPubMedGoogle Scholar
  2. Koci  MD, Schultz-Cherry  S. Avian astroviruses. Avian Pathol. 2002;31:213–27. DOIPubMedGoogle Scholar
  3. Moser  LA, Schultz-Cherry  S. Pathogenesis of astrovirus infection. Viral Immunol. 2005;18:4–10. DOIPubMedGoogle Scholar
  4. Caracciolo  S, Minini  C, Colombrita  D, Foresti  I, Avolio  M, Tosti  G, Detection of sporadic cases of Norovirus infection in hospitalized children in Italy. New Microbiol. 2007;30:49–52.PubMedGoogle Scholar
  5. Glass  RI, Noel  J, Mitchell  D, Herrmann  JE, Blacklow  NR, Pickering  LK, The changing epidemiology of astrovirus-associated gastroenteritis: a review. Arch Virol Suppl. 1996;12:287–300.PubMedGoogle Scholar
  6. Kirkwood  CD, Clark  R, Bogdanovic-Sakran  N, Bishop  RF. A 5-year study of the prevalence and genetic diversity of human caliciviruses associated with sporadic cases of acute gastroenteritis in young children admitted to hospital in Melbourne, Australia (1998–2002). J Med Virol. 2005;77:96–101. DOIPubMedGoogle Scholar
  7. Klein  EJ, Boster  DR, Stapp  JR, Wells  JG, Qin  X, Clausen  CR, Diarrhea etiology in a children’s hospital emergency department: a prospective cohort study. Clin Infect Dis. 2006;43:807–13. DOIPubMedGoogle Scholar
  8. Soares  CC, Maciel de Albuquerque  MC, Maranhao  AG, Rocha  LN, Ramirez  ML, Benati  FJ, Astrovirus detection in sporadic cases of diarrhea among hospitalized and non-hospitalized children in Rio de Janeiro, Brazil, from 1998 to 2004. J Med Virol. 2008;80:113–7. DOIPubMedGoogle Scholar
  9. Finkbeiner  SR, Allred  AF, Tarr  PI, Klein  EJ, Kirkwood  CD, Wang  D. Metagenomic analysis of human diarrhea: viral detection and discovery. PLoS Pathog. 2008;4:e1000011. DOIPubMedGoogle Scholar
  10. Finkbeiner  SR, Kirkwood  CD, Wang  D. Complete genome sequence of a highly divergent astrovirus isolated from a child with acute diarrhea. Virol J. 2008;5:117.PubMedGoogle Scholar
  11. Noel  JS, Lee  TW, Kurtz  JB, Glass  RI, Monroe  SS. Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing. J Clin Microbiol. 1995;33:797–801.PubMedGoogle Scholar
  12. Swofford  DL. PAUP*: phylogenetic analysis using parsimony (*and other methods). Version 4. Sunderland (MA): Sinauer Associates; 1998.
  13. Chiu  CY, Greninger  AL, Kanada  K, Kwok  T, Fischer  KF, Runckel  C, Identification of cardioviruses related to Theiler's murine encephalomyelitis virus in human infections. Proc Natl Acad Sci U S A. 2008;105:14124–9. DOIPubMedGoogle Scholar

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