Volume 15, Number 8—August 2009
Research
Entomologic and Virologic Investigation of Chikungunya, Singapore
Lee-Ching Ng
, Li-Kiang Tan, Cheong-Huat Tan, Sharon S.Y. Tan, Hapuarachchige C. Hapuarachchi, Kwoon-Yong Pok, Yee-Ling Lai, Sai-Gek Lam-Phua, Göran Bucht, Raymond T.P. Lin, Yee-Sin Leo, Boon-Hian Tan, Hwi-Kwang Han, Peng-Lim S Ooi, Lyn James, and Seow-Poh Khoo
, Li-Kiang Tan, Cheong-Huat Tan, Sharon S.Y. Tan, Hapuarachchige C. Hapuarachchi, Kwoon-Yong Pok, Yee-Ling Lai, Sai-Gek Lam-Phua, Göran Bucht, Raymond T.P. Lin, Yee-Sin Leo, Boon-Hian Tan, Hwi-Kwang Han, Peng-Lim S Ooi, Lyn James, and Seow-Poh Khoo
Table 1
Primers for DNA template synthesis and sequencing of chikungunya virus, Singapore*
| Name/genomic position† | Sequence (5′ → 3′) |
|---|---|
| ChikE1/9870F | ACAAGCCCTTATTCCGCTG |
| ChikE1/9994F | TACGAACACGTAACAGTGATC |
| ChikE1/10246F | TACCCATTTATGTGGGGC |
| ChikE1/10378F | GCATCAGCTAAGCTCCGC |
| ChikE1/10397R | ACGCGGAGCTTAGCTGAT |
| ChikE1/10521R | ACCTTTGTACACCACAATT |
| ChikE1/10643F | CACAACTGGTACTGCAGAGACC |
| ChikE1/10710R | GCCAGATGGTGCCTGAGA |
| ChikE1/10965F | GAAAGGCAAGTGTGCGGT |
| ChikE1/10993R | TCATCGAATGCACCGCAC |
| ChikE1/11232F | CACGGGAGGTGTGGGAC |
| ChikE1/11238R | TCCCGTGATCTTCTGCACC |
| ChikE1/11359R | GTGTGTCTCTTAGGGGACACATA |
*Chik, chikungunya; F, forward primer; R, reverse primer.
†Genomic position of chikungunya virus (GenBank accession no. DQ443544.2) to which the first base (5′ end) of the primer corresponds.
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