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Volume 16, Number 1—January 2010
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Human Group A Streptococci Virulence Genes in Bovine Group C Streptococci

Márcia G. Rato, Ricardo Bexiga, Sandro F. Nunes, Cristina L. Vilela, and Ilda Santos-SanchesComments to Author 
Author affiliations: Universidade Nova de Lisboa, Caparica, Portugal (M.G. Rato, I. Santos-Sanches); Universidade Técnica de Lisboa, Lisbon, Portugal (R. Bexiga, S.F. Nunes, C.L. Vilela); Cambridge University, Cambridge, UK (S.F. Nunes)

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Figure 1

Dendrogram and pulsed-field gel electrophoresis (PFGE) profiles of group C streptococci (Streptococcus dysgalactiae subsp. dysgalactiae) subclinical mastitis isolates from 8 dairy herds, Portugal. PFGE type-subtype, virulence genotype, antimicrobial drug resistance phenotypes, and genotypes of each isolate are indicated. The dendrogram was produced by using Dice coefficients and unweighted pair group method using arithmetic averages. Default clustering settings of 0.00% optimization (i.e., the r

Figure 1. Dendrogram and pulsed-field gel electrophoresis (PFGE) profiles of group C streptococci (Streptococcus dysgalactiae subsp. dysgalactiae) subclinical mastitis isolates from 8 dairy herds, Portugal. PFGE type-subtype, virulence genotype, antimicrobial drug resistance phenotypes, and genotypes of each isolate are indicated. The dendrogram was produced by using Dice coefficients and unweighted pair group method using arithmetic averages. Default clustering settings of 0.00% optimization (i.e., the relative distance an entire lane is allowed to shift in matching attempts) and 1.5% band position tolerance were used. *All isolates were negative for speA, ssa, speH, speJ, speI, and slaA genes and for Tn1207.3/Φ10394.4 element right junction tested by PCR; **All isolates were negative for mefA, tet(T), tet(W), tet(L), tet(Q), tet(S) and tet(K) genes tested by PCR; TET, resistance only to tetracycline; MLSB-TET, resistance to macrolides, lincosamides, streptogramin B, and TET; L-TET, susceptibility to macrolides and resistance to lincosamides (L phenotype) and TET; Tn1207.3 LJ, Tn1207.3/Φ10394.4 element left junction. Clusters are shown in roman numerals on the right.

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