Volume 16, Number 6—June 2010
Letter
Laboratory Diagnosis of Lassa Fever, Liberia
Table
Laboratory results and primer sequences on 12 patients with Lassa fever, Liberia*†
Strain designation‡ | Deviations from sequence (5′ → 3′)‡ |
Original primers (6) | Modified primers | Cell culture | Log10 RNA copies/mL | Anti-LASV IgM titer‡ | Anti-LASV IgG titer‡ |
---|---|---|---|---|---|---|---|
TGCACAAAGAACAACAGTCATCATTATAT | |||||||
129/05 | ---T----A-----T-----C-------- | – | + | + | 3.20 | Neg | <10 |
UN133 | ---T----A-----T-----C-------- | – | + | + | 3.99 | Neg | <10 |
121/1580 | --T-----A-----------C--C----- | – | + | + | 4.14 | Neg | <10 |
127/05 | --------A-------------------- | + | + | + | 6.22 | Neg | <10 |
4094/05 | --------A-----------------C-- | + | + | + | 4.53 | Neg | <10 |
Lib3800 | --T-----------------C-------- | + | + | + | 4.90 | 160 | 5,120 |
Lib88 | --T-----------------C—-C----- | + | + | + | 7.49 | 40 | 20 |
Lib90 | --T-----------------C—-C----- | + | + | + | 5.17 | Neg | <10 |
295/06 | --T-----------------C—-C----- | + | + | + | 5.41 | 80 | 640 |
383/06 | --------------------C--C--C-- | + | + | + | 4.38 | 40 | <10 |
120/06 | --------A--------C--C-----C-- | + | + | + | 4.50 | Neg | <10 |
174/06 | -----------TG-T--C--C--C--C-- | + | + | + | 5.02 | 320 | 640 |
*LASV, Lassa fever virus; Ig, immunoglobulin.
†Primers sequences are compared to that published by Demby et al. (6) (reverse primer binding sites). Virus isolation was done on Vero cells in a BioSafety level 4 laboratory. Serologic testing for anti-LASV IgM and IgG was performed by using the indirect immunofluorescent assay.
‡From reference strain AY628203, position 3092–3064 = reverse primer binding site in (6); note that the forward primer is not analyzed because it is located in the conserved stem-loop structure of LASV small segment RNA.
References
- McCormick JB, Webb PA, Krebs JW, Johnson KM, Smith ES. A prospective study of the epidemiology and ecology of Lassa fever. J Infect Dis. 1987;155:437–44.PubMedGoogle Scholar
- Ter Meulen J, Koulemou K, Wittekindt T, Windisch K, Strigl S, Conde S, Detection of Lassa virus antinucleoprotein immunoglobulin G (IgG) and IgM antibodies by a simple recombinant immunoblot assay for field use. J Clin Microbiol. 1998;36:3143–8.PubMedGoogle Scholar
- Fair J, Jentes E, Inapogui A, Kourouma K, Goba A, Bah A, Lassa virus–infected rodents in refugee camps in Guinea: a looming threat to public health in a politically unstable region. Vector Borne Zoonotic Dis. 2007;7:167–71. DOIPubMedGoogle Scholar
- Khan SH, Goba A, Chu M, Roth C, Healing T, Marx A, New opportunities for field research on the pathogenesis and treatment of Lassa fever. Antiviral Res. 2008;78:103–15. DOIPubMedGoogle Scholar
- Drosten C, Kummerer BM, Schmitz H, Gunther S. Molecular diagnostics of viral hemorrhagic fevers. Antiviral Res. 2003;57:61–87. DOIPubMedGoogle Scholar
- Drosten C, Gottig S, Schilling S, Asper M, Panning M, Schmitz H, Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription–PCR. J Clin Microbiol. 2002;40:2323–30. DOIPubMedGoogle Scholar
- Trappier SG, Conaty AL, Farrar BB, Auperin DD, McCormick JB, Fisher-Hoch SP. Evaluation of the polymerase chain reaction for diagnosis of Lassa virus infection. Am J Trop Med Hyg. 1993;49:214–21.PubMedGoogle Scholar
- Bowen MD, Rollin PE, Ksiazek TG, Hustad HL, Bausch DG, Demby AH, Genetic diversity among Lassa virus strains. J Virol. 2000;74:6992–7004. DOIPubMedGoogle Scholar
- Bausch DG, Rollin PE, Demby AH, Coulibaly M, Kanu J, Conteh AS, Diagnosis and clinical virology of Lassa fever as evaluated by enzyme-linked immunosorbent assay, indirect fluorescent-antibody test, and virus isolation. J Clin Microbiol. 2000;38:2670–7.PubMedGoogle Scholar