Volume 16, Number 6—June 2010
Dispatch
Pneumovirus in Dogs with Acute Respiratory Disease
Randall W. Renshaw, Nancy C. Zylich, Melissa A. Laverack, Amy L. Glaser, and Edward J. Dubovi
Table
Partial gene locations of virus isolated from dogs with respiratory disease and relatedness to other pneumoviruses*
| Region sequenced† | PCR primers (5′ → 3′) and aa target sequences in pneumoviruses | % Identity to MPV, nt, aa† | % Identity to HRSV, nt, aa‡ | % Identity to HMPV, nt, aa§ |
|---|---|---|---|---|
| N gene,¶ nt 852–1182 (331 bp) |
P1R: ggaactcgggggcgaayttytccat
target: MEKFAPEFH
N276F: tccgtgcaggccgaratggarcarg
target: SVQAEMEQV |
96.4, 96.3 |
68.5, 70.1 |
56.5, 54.1 |
| L gene, no. 1,¶ nt 1143–1452 (310 bp) |
L428F: ccggatcttcggccayccnatggt
target: RIFGHPMV
L538R: ttcttaggaggggagatggcyttrtcrtt
target: NDKAISPPKN |
95.8, 97.1 |
62.1, 55.3 |
58.9, 47.6 |
| L gene, no. 2,¶ nt 1962–2511 (550 bp) | L698F: catcaccgacctgtccaagttyaaycargc target: ITDLSKFNQA L894R: ttgaagtcgtccaggatggtrttdatcca target: WINTILDDFK | 96.7, 99.5 | 68.4, 71.6 | 66.4, 68.3 |
*MPV, murine pneumovirus; nt, nucleotide; aa, amino acid; HRSV, human respiratory syncytial virus; HMPV, human metapneumovirus; N, nucleocapsid; R, reverse primer; F, forward primer L, polymerase.
†Based on comparison with MPV strain J3666, GenBank accession no. NC_006579.
‡Based on comparison with HRSV, GenBank accession no. NC_001803.
§Based on comparison with HMPV, GenBank accession no. NC_004148.
¶The N gene fragment is entered under GenBank accession no. GU247050; L gene fragments nos. 1 and 2 are entered under GenBank accession no. GU247051.
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