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Volume 8, Number 11—November 2002
THEME ISSUE
Tuberculosis Genotyping
Tuberculosis Genotyping Network, United States

Molecular Differentiation of Mycobacterium tuberculosis Strains without IS6110 Insertions

Kerry H. Lok*, William H. Benjamin*, Michael E. Kimerling*Comments to Author , Virginia Pruitt†, Monica Lathan‡, Jafar Razeq‡, Nancy Hooper‡, Wendy A. Cronin‡, and Nancy E. Dunlap*
Author affiliations: *University of Alabama at Birmingham, School of Medicine, Birmingham, Alabama, USA; †Alabama Department of Public Health, Montgomery, Alabama, USA; ‡Maryland Department of Health and Mental Hygiene, Baltimore, Maryland, USA;

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Table

Zero-copy IS6110 Mycobacterium tuberculosis strains from previously reported studies, other studies from the National Tuberculosis Genotyping Surveillance Network, and strains from current studya

No./case Spoligotype octal description CDC designation Country of origin Yr reported Source of data
1 N/A N/A India 1993 (10)
3 N/A N/A Hong Kong 1995 (5)
5 N/A N/A India 1995 (5)
3 577767000000011 N/A Asia 1997 (12)
1 777777777760771 0002 Curacao 1999 (13)
1 477776501013071 N/A India 1999 (13)
1 777701002001731 N/A the Netherlands 1999 (13)
1 N/A N/A United States Unpublished Arkansas
1 777777777760700 0202 China Unpublished California
8 777777000000011 0258 Vietnam Unpublished California
1 777603000000011 1682 Vietnam Unpublished California
1 777777600007771 1128 North Korea Unpublished California
1 777777000000011 0258 Vietnam Unpublished Michigan
1 777603002000011 0870 Vietnam Unpublished Texas
1 N/A N/A Vietnam Unpublished Texas
1 000000000003771 0034 N/A 2000 (17)
2 777777000000011 0258 Vietnam 2001 (18)
1 777603000000011 1682 Vietnam 2001 (18)
1 741777000000011 n/a Vietnam 2001 (18)
2 777647000000011 n/a United States 2001 (18)
3 777777000000011 0258 Vietnam 2002 Study isolates 1–3
1 700100000000731 0968 Iraq 2002 Study isolates 4
1 577600000000000 0742 India 2002 Study isolates 5
1 777777777763771 0169 Liberia 2002 Study isolates 6
43

aCDC; Centers for Disease Control and Prevention.

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References
  1. Mazurek  GH, Cave  MD, Eisenach  KD, Wallace  RJJ, Bates  JH, Crawford  JT. Chromosomal DNA fingerprint patterns produced with IS6110 as strain-specific markers for epidemiologic study of tuberculosis. J Clin Microbiol. 1991;29:2030–3.PubMedGoogle Scholar
  2. Dunlap  NE. The use of RFLP as a tool for tuberculosis control: utility or futility? Int J Tuberc Lung Dis. 2000;4:S134–8.PubMedGoogle Scholar
  3. Small  PM, Hopewell  PC, Singh  SP, Paz  A, Parsonnet  J, Ruston  DC, The epidemiology of tuberculosis in San Francisco. A population-based study using conventional and molecular methods. Stability of DNA fingerprint pattern produced with IS6110 in strains of Mycobacterium tuberculosis. N J Med. 1994;330:1703–9. DOIGoogle Scholar
  4. Jasmer  R, Hahn  J, Small  P, Daley  C, Behr  M, Moss  A, A molecular epidemiologic analysis of tuberculosis trends in San Francisco, 1991–1997. Ann Intern Med. 1999;130:971–8.PubMedGoogle Scholar
  5. Das  S, Paramasivan  CN, Lowrie  DB, Prabhakar  R, Narayanan  PR. IS6110 restriction fragment length polymorphism typing of clinical isolates of Mycobacterium tuberculosis from patients with pulmonary tuberculosis in Madras, south India. Tuber Lung Dis. 1995;76:550–4. DOIPubMedGoogle Scholar
  6. Das  S, Chan  SL, Allen  BW, Mitchison  DA, Lowrie  DB. Application of DNA fingerprinting with IS986 to sequential mycobacterial isolates obtained from pulmonary tuberculosis patients in Hong Kong before, during and after short-course chemotherapy. Tuber Lung Dis. 1993;74:47–51. DOIPubMedGoogle Scholar
  7. van Embden  JD, Cave  MD, Crawford  JT, Dale  JW, Eisenach  KD, Gicquel  B, Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol. 1993;31:406–9.PubMedGoogle Scholar
  8. Groenen  PM, Bunschoten  AE, van Soolingen  D, van Embden  JD. Nature of DNA polymorphism in the direct repeat cluster of Mycobacterium tuberculosis; application for strain differentiation by a novel typing method. Mol Microbiol. 1993;10:1057–65. DOIPubMedGoogle Scholar
  9. Dale  JW, Brittain  D, Cataldi  AA, Cousins  D, Crawford  JT, Driscoll  J, Spacer oligonucleotide typing of bacteria of the Mycobacterium tuberculosis complex: recommendations for standardised nomenclature. Int J Tuberc Lung Dis. 2001;5:216–9.PubMedGoogle Scholar
  10. van Soolingen  D, de Haas  P, Hermans  P, Groenen  P, van Embden  JD. Comparison of various repetitive DNA elements as genetic markers for strain differentiation and epidemiology of Mycobacterium tuberculosis. J Clin Microbiol. 1993;31:1987–95.PubMedGoogle Scholar
  11. Frothingham  R, Meeker-O'Connell  WA. Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats. Microbiology. 1998;144:1189–96. DOIPubMedGoogle Scholar
  12. Goguet de la Salmoniere  YO, Li  HM, Torrea  G, Bunschoten  A, van Embden  J, Gicquel  B. Evaluation of spoligotyping in a study of the transmission of Mycobacterium tuberculosis. J Clin Microbiol. 1997;35:2210–4.PubMedGoogle Scholar
  13. Kremer  K, van Soolingen  D, Frothingham  R, Haas  WH, Hermans  PW, Martin  C, Comparison of methods based on different molecular epidemiological markers for typing of Mycobacterium tuberculosis complex strains: interlaboratory study of discriminatory power and reproducibility. J Clin Microbiol. 1999;37:2607–18.PubMedGoogle Scholar
  14. Soini  H, Pan  X, Teeter  L, Musser  JM, Graviss  EA. Transmission dynamics and molecular characterization of Mycobacterium tuberculosis isolates with low copy numbers of IS6110. J Clin Microbiol. 2001;39:217–21. DOIPubMedGoogle Scholar
  15. Sola  C, Horgen  L, Goh  KS, Rastogi  N. Molecular fingerprinting of Mycobacterium tuberculosis on a Caribbean island with IS6110 and DRr probes. J Clin Microbiol. 1997;35:843–6.PubMedGoogle Scholar
  16. Beggs  ML, Eisenach  KD, Cave  MD. Mapping of IS6110 insertion sites in two epidemic strains of Mycobacterium tuberculosis. J Clin Microbiol. 2000;38:2923–8.PubMedGoogle Scholar
  17. Soini  H, Pan  X, Amin  A, Graviss  EA, Siddiqui  A, Musser  JM. Characterization of Mycobacterium tuberculosis isolates from patients in Houston, Texas, by spoligotyping. J Clin Microbiol. 2000;38:669–76.PubMedGoogle Scholar
  18. Soini  H, Musser  JM. Molecular diagnosis of mycobacteria. Clin Chem. 2001;47:809–14.PubMedGoogle Scholar

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