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Volume 9, Number 2—February 2003
Research

Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates

Wieland Meyer*Comments to Author , Alexandra Castañeda†, Stuart Jackson*‡, Matthew Huynh*, Elizabeth Castañeda†, and the IberoAmerican Cryptococcal Study Group
Author affiliations: *University of Sydney, Sydney, Australia; †Instituto Nacional de Salud, Bogotá, Colombia; ‡University of Western Sydney, Campbelltown, Australia

Main Article

Figure 3

Polymerase chain reaction (PCR) fingerprints generated with the primer M13 (2A), and URA5 gene restriction fragment length polymorphism (RFLP) profiles identified by double digest of the gene with Sau96I and HhaI (2B) obtained from a selection of Mexican Cryptococcus neoformans isolates, given as a representative example for the patterns obtained from clinical and environmental isolates from Latin America. Standard patterns obtained from the reference strains of the major molecular types by PCR-

Figure 3. Polymerase chain reaction (PCR) fingerprints generated with the primer M13 (2A), and URA5 gene restriction fragment length polymorphism (RFLP) profiles identified by double digest of the gene with Sau96I and HhaI (2B) obtained from a selection of Mexican Cryptococcus neoformans isolates, given as a representative example for the patterns obtained from clinical and environmental isolates from Latin America. Standard patterns obtained from the reference strains of the major molecular types by PCR-fingerprinting patterns with the microsatellite specific primer M13 as a single primer in the PCR (right-hand side of 2A) and URA5 gene RFLP profiles generated after double digest with Sau96I and HhaI (right-hand side of 2B) (VNIII correspond to the seven-band URA5 RFLP pattern and VNIII* correspond to the six-band URA5 RFLP pattern).

Main Article

1Members of the IberoAmerican Cryptococcal Study Group: Argentina: Alicia Arechavala, Hospital de Infecciosas Francisco J. Muñiz, Buenos Aires; Graciela Davel, Laura Rodero, Diego Perrotta, Departamento Micología, Instituto Nacional de Enfermedades Infecciosas “Dr. Carlos Gregorio Malbrán,” Buenos Aires; Brazil: Marcia Lazera, Ricardo Pereira-Igreja, Bodo Wanke, Laboratorio de Micologia Medica, Hospital Evandro Chagas, Fundaςao Oswaldo Cruz, Rio de Janeiro; Maria Jose Mendes-Giannini, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Araraquara; Marcia S.C. Melhem, Adolfo Lutz Institute Seςao de Micologia, São Paulo; Marlene Henning-Vainstein, Centro de Biotecnologia (UFRGS), Porto Alegre; Chile: Maria Cristina Díaz, Programa de Microbiología y Micología, Universidad de Chile, Santiago; Colombia: Angela Restrepo, Corporación para Investigaciones Biológicas, Medellín; Sandra Huérfano, Instituto Nacional de Salud, Bogotá; Guatemala: Blanca Samayoa, Hospital San Juan de Dios, Guatemala City; Heidi Logeman, Universidad de San Carlos, Guatemala City; Mexico: Rubén López Martínez, Laura Rocío Castañon Olivares, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México City; Cudberto Contreras-Peres, José Francisco Valenzuela Tovar, Instituto Nacional de Diagnóstico y Referencia Epidemiológicos , Mexico City; Peru: Beatriz Bustamante, Instituto de Medicina Tropical Alexander Humboldt, Lima; Spain: Joseph Torres-Rodriquez, Yolanda Morera, Grup de recerca en Micologia Experimental i Clínica, Institut Municipal d’Investigació Médica, Univesitat Autonoma de Barcelona, Barcelona; Venezuela: Belinda Calvo, Universidad del Zulia, Maracaibo

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