Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

Volume 10, Number 11—November 2004


Cholera in Mozambique, Variant of Vibrio cholerae1

Cite This Article


Highlight and copy the desired format.

EID Ansaruzzaman M, Bhuiyan N, Nair GB, Sack DA, Lucas M, Deen JL, et al. Cholera in Mozambique, Variant of Vibrio cholerae. Emerg Infect Dis. 2004;10(11):2057-2059.
AMA Ansaruzzaman M, Bhuiyan N, Nair GB, et al. Cholera in Mozambique, Variant of Vibrio cholerae. Emerging Infectious Diseases. 2004;10(11):2057-2059. doi:10.3201/eid1011.040682.
APA Ansaruzzaman, M., Bhuiyan, N., Nair, G. B., Sack, D. A., Lucas, M., Deen, J. L....Chaignat, C. (2004). Cholera in Mozambique, Variant of Vibrio cholerae. Emerging Infectious Diseases, 10(11), 2057-2059.

To the Editor: Cholera outbreaks caused by toxigenic Vibrio cholerae serogroup O1 frequently occur in many sub-Saharan African countries. The serogroup O1 is classified into two biotypes, classical and El Tor. The seventh and current pandemic of cholera is caused by the El Tor biotype; the classical biotype is believed to be extinct. The classical and El Tor biotypes of V. cholerae O1 are closely related in their O-antigen biosynthetic genes but differ in other regions of the genome. The genomic structure of the CTXΦ filamentous phage (1), in which the cholera toxin genes are contained, differs between the classical and El Tor biotypes. CTXclassΦ is found in classical strains, CTXETΦ is present in El Tor and O139 strains, and CTXcalcΦ is found in resurgent O139 strains. The diversity of CTXΦ among biotypes is mainly due to the variations in the repeat sequence elements, particularly in the rstR gene region (2).

While conducting surveillance in the cholera treatment center in Beira, the second largest city in Mozambique, we examined 175 rectal swabs or stool samples from January 7 to March 8, 2004, using standard published procedures. During this period, we isolated 58 strains of V. cholerae O1. The isolates were transported to the Enteric Microbiology Unit of the International Center for Diarrhoeal Disease Research in Dhaka, Bangladesh (ICDDRD,B), for further phenotypic and genotypic characterization to determine serotype, biotype, and presence of important virulence genes. All 58 strains were identified as V. cholerae O1 of the Ogawa serotype. Forty strains selected for detailed characterization were resistant to polymyxin B, agglutinated chicken cells, yielded a positive Voges-Proskauer reaction, were positive for the El Tor hemolysin by the tube agglutination method, and were sensitive to group IV El Tor phage but resistant to the classical group V phage and were therefore classified as the El Tor biotype. The antimicrobial susceptibility of 15 of the 40 isolates examined showed that the strains were sensitive to tetracycline, ampicillin, furazolidone, erythromycin, and ciprofloxacin but resistant to trimethoprim-sulfamethoxazole, and also to the vibriostatic compound 0/129.


Thumbnail of Amino acid sequence alignment of CT-B subunit of Vibrio cholerae O1 classical, El Tor, and Mozambique (B33 and B65) strains. Identical amino acid residues are indicated by a period. Amino acid sequences of ctxB of V. cholerae classical (AAL60524.1; AAM47189.1) and El Tor (AAM74192.1; AAM77066.1) are from GenBank.

Figure. Amino acid sequence alignment of CT-B subunit of Vibrio cholerae O1 classical, El Tor, and Mozambique (B33 and B65) strains. Identical amino acid residues are indicated by a period. Amino acid...

By using polymerase chain reaction (PCR), we established that all 40 strains carried the ctxA gene (constituent gene of the CTX prophage) and the tcpA gene (the El Tor type), a constituent gene of the vibrio pathogenicity island. We then focused on the rstR gene because of its diversity between the two biotypes. All of the 40 El Tor strains produced a 500-bp PCR product of the rstR gene of the classical type (rstRclass), despite belonging to the El Tor biotype. Nucleotide sequence analysis of the rstR gene of two representative Mozambique strains showed 100% homology to the classical rstR gene of classical reference strain O395. The amino acid sequence of the B-subunit of classical and El Tor biotypes have distinct signature sequences (3). We amplified the ctxB gene using specific primers and found that the deduced amino acid sequence of the CT-B subunit of the Mozambique strains varied from the El Tor CT-B subunit at positions 39 (histidine replaces tyrosine in El Tor) and 68 (threonine replaces isoleucine in El Tor), and the amino acid residues at these positions are identical to those of the classical CT-B subunit (Figure). The nucleotide sequences obtained for ctxB of the two Mozambique strains B33 and B65 were deposited in GenBank under accession numbers AY648939 and AY6448940, respectively. Therefore, the Mozambique strains of V. cholerae O1 displayed typical traits of the El Tor biotype overall but carried the classical CTX prophage.

Our findings that El Tor strains of V. cholerae O1 from Mozambique are carrying the classical prophage shows the presence of genetic materials associated with the classical biotype in Mozambique. Further, these findings provide the first circumstantial evidence of transmission of the classical CTX prophage. The CTX prophages in El Tor strains give rise to infectious phage particles (1), but neither of the two CTX prophages integrated at two different sites of the classical genome give rise to phage particles (4). Subsequent studies have shown that, although the genes of the classical prophages encode functional forms of all of the proteins needed for production of CTXΦ, the CTX prophage does not yield virions because of the atypical arrangement of its prophage arrays (4).

Genetic hybrids between El Tor and classical biotypes of O1 V. cholerae were reported among sporadic isolates earlier in Bangladesh (5) and were named the Matlab variants after the place where they were first isolated. The Mozambique strains of V. cholerae likely evolved from an El Tor strain, which shed its CTX phage and acquired the classical prophage. Alternatively, strains like the Matlab variant may have spread to the African subcontinent. Whether introducing the CTX prophage in the El Tor genome background will increase pathogenicity, affect genomic stability, or enhance the epidemic-causing potential is uncertain. This subtle genetic change might also alter the effectiveness of current cholera vaccines which stimulate antitoxic as well as antibacterial immunity.


We thank J.J. Mekalanos for useful comments on this manuscript and Motiur Rahman for help with the nucleotide sequencing. We also thank the International Vaccine Institute funded by the Bill and Melinda Gates Foundation (Diseases of the Most Impoverished Program).

The ICDDR,B is supported by the aid agencies of the governments of Australia, Bangladesh, Belgium, Canada, Japan, Kingdom of Saudi Arabia, the Netherlands, Sweden, Sri Lanka, Switzerland, and the United States.

M. Ansaruzzaman*, N.A. Bhuiyan*, G. Balakrish Nair*, David A. Sack*Comments to Author , Marcelino Lucas†, Jacqueline L. Deen‡, Julia S. Ampuero§¶, Claire-Lise Chaignat#, and The Mozambique Cholera Vaccine Demonstration Project Coordination Group

Author affiliations: *Centre for Health and Population Research, Dhaka, Bangladesh; †Ministério da Saude, Maputo, Mozambique; ‡International Vaccine Institute, Seoul, Korea; §Médecins Sans Frontières, Geneva, Switzerland; ¶Epicentre, Paris, France; #World Health Organization, Geneva, Switzerland


  1. Waldor MK, Mekalanos JJ. Lysogenic conversion by a filamentous phage encoding cholera toxin. Science. 1996;272:19104. DOIPubMed
  2. Kimsey HH, Nair GB, Ghosh A, Waldor MK. Diverse CTXΦ and evolution of new pathogenic Vibrio cholerae. Lancet. 1998;352:4578. DOIPubMed
  3. Popovic T, Fields PI, Olsvik O. Detection of cholera toxin genes. In: Wachsmuth IK, Blake PA, Olsvik O, editors. Vibrio cholerae and cholera: molecular to global perspectives. Washington: American Society for Microbiology; 1994. p. 41–52.
  4. Davis BM, Waldor MK. Filamentous phages linked to virulence of Vibrio cholerae. Curr Opin Microbiol. 2003;6:3542. DOIPubMed
  5. Nair GB, Faruque SM, Bhuiyan NA, Kamruzzaman M, Siddique AK, Sack DA. New variants of Vibrio cholerae O1 biotype El Tor with attributes of the classical biotype from hospitalized patients with acute diarrhea in Bangladesh. J Clin Microbiol. 2002;40:32969. DOIPubMed


Cite This Article

DOI: 10.3201/eid1011.040682

1The group includes participants from the: Ministério da Saude, Maputo, Mozambique (Avertino Barreto, Juvenaldo Amos, Catarina Mondlane, and Raul Vaz); International Vaccine Institute (John D. Clemens, Lorenz von Seidlein, Xuan-Yi Wang, Mohammad Ali, and Mahesh K Puri); Médecins Sans Frontières, Geneva, Switzerland (Claude Mahoudeau, Bruno Lab, Gérard Bedock, Valerie Perroud, and Margaret McChesney); Epicentre, Paris, France (Philippe J. Guerin, Dominique Legros, and Philippe Cavailler); World Health Organization, Geneva, Switzerland (Marie-Paule Kieny and Duncan Steele); and World Health Organization, Maputo, Mozambique (Bocar Touré and Pierre Kahozi).

Related Links

Table of Contents – Volume 10, Number 11—November 2004


Please use the form below to submit correspondence to the authors or contact them at the following address:

David Sack, Executive Director, ICDDR,B, Centre for Health and Population Research and Mohakhali, Dhaka 1000, Bangladesh; fax: 880-2-882-3116David Sack, Executive Director, ICDDR,B, Centre for Health and Population Research and Mohakhali, Dhaka 1000, Bangladesh; fax: 880-2-882-3116

character(s) remaining.

Comment submitted successfully, thank you for your feedback.