Volume 11, Number 10—October 2005
Dispatch
Rapid West Nile Virus Antigen Detection
Nicholas A. Panella*
, Kristen L. Burkhalter*, Stanley A. Langevin*, Aaron C. Brault*, Lynn M. Schooley*, Brad J. Biggerstaff*, Roger S. Nasci*, and Nicholas Komar*
, Kristen L. Burkhalter*, Stanley A. Langevin*, Aaron C. Brault*, Lynn M. Schooley*, Brad J. Biggerstaff*, Roger S. Nasci*, and Nicholas Komar*
Table 2
Log geometric mean titer (SD) of West Nile virus PFU equivalents (TaqMan RT-PCR) and PFU (plaque assay) for American Crows and House Sparrows, by swab type and day postmortem*
| Species | Swab type | Assay | Day postmortem |
|||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | |||
| American Crow | Oral | TaqMan | 4.8 (1.1) | 4.7 (0.6) | 4.3 (0.8) | 3.5 (1.5) |
| Plaque | 3.6 (1.9) | 3.2 (1.5) | 2.4 (1.6) | 2.2 (2.0) | ||
| Cloacal | TaqMan | 5.2 (1.2) | 5.5 (1.3) | 4.6 (1.9) | 3.5 (0.7) | |
| Plaque | 3.0 (2.4) | 1.4 (1.5) | 0.3 (0.7) | 0.0 (0.0) | ||
| House Sparrow | Oral | TaqMan | 4.3 (1.4) | 5.0 (1.7) | 4.6 (1.7) | NA |
| Plaque | 3.8 (2.0) | 3.0 (1.9) | 2.9 (2.5) | NA | ||
| Cloacal | TaqMan | 3.8 (1.6) | 4.3 (3.3) | NA | NA | |
| Plaque | 1.9 (2.9) | 0.7 (0.9) | NA | NA | ||
*RT-PCR, reverse transcription–polymerase chain reaction; NA, not available (valid sampling was not possible given the physical state of the bird).
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