Volume 11, Number 10—October 2005
Research
Pyrosequencing Bacillus anthracis
Tara Wahab*, Sandra Hjalmarsson*, Ralfh Wollin*, and Lars Engstrand*
Table 2
Sequence of primers used in PCR and sequencing*
| Target | Primer | Sequence |
|---|---|---|
| rpoB | PCR forward | 5´-CCACCAACAGTAGAAAATGCCA-3´ |
| PCR reverse | b-5´-AAATTTCACCAGTTTCTGGATCTA-3´ | |
| Sequencing 911 | 5´-CACCAACAGTAGAAAATGC-3´ | |
| Sequencing 912 | 5´-TTCTTCGATCCAAAGC-3´ | |
| Sequencing 913 | 5´-TAAAAACAGATTGTTTA-3´ | |
| Sequencing 914 | 5´-AACGTTTAGCTGAAACATT-3´ | |
| pXO1 | PCR forward | 5´-GATATGAACCCGTACTTGTAAT-3´ |
| PCR reverse | b-5´-GAATCAGATGCATTTTTAATGG-3´ | |
| Sequencing | 5´-TTTATTATGAAATAGGT-3´ | |
| pXO2 | PCR forward | 5´-TTCGCCTTGTGATTCCGCACT-3´ |
| PCR reverse | b-5´-GGGCGCTGGTCGTATGTTTAG-3´ | |
| Sequencing | 5´- TGTTTGTCCACTTCC-3´ |
*PCR, polymerase chain reaction; b, biotin.
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