Figure 4. Capillary electrophoresis–single strand conformation polymorphisms (CE-SSCP) for the identification of varieties and species. The ABI PRISM 310 Genetic Analyzer and GeneScan analysis software were used for variety and species determination with the MFα1 pheromone gene. The MFα1 sense and antisense primers were labeled with fluorescent probes FAM (blue) and TET (green), and polymerase chain reaction amplicons were analyzed with 3% polymer at 30°C under nondenaturing conditions. The blue and green peaks depict characteristic peak pattern for Cryptococcus neoformans var. grubii (CnVG), Cryptococcus neoformans var. neoformans (CnVN), and Cryptococcus gattii (Cg). These peaks were aligned by using an internal size standard.