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Volume 11, Number 12—December 2005

Porcine Noroviruses Related to Human Noroviruses

Qiu-Hong Wang*, Myung Guk Han*, Sonia Cheetham*, Menira Souza*, Julie A. Funk†, and Kwonil Jung*Comments to Author 
Author affiliations: *The Ohio State University, Wooster, Ohio, USA; †The Ohio State University, Columbus, Ohio, USA

Main Article

Table 4

Antigenic cross-reactivity between human GII NoV antigens (VLPs) and a pig convalescent-phase antiserum against porcine GII NoVs, as determined by ELISA*

Antiserum ELISA antibody titer with each VLP antigen (genogroup-genotype)
(GII-1) Toronto (GII-3) MD145 (GII-4) HS66 (GII-4) Florida (GII-6) Desert Shield (GI-3)
HS66CS (positive control): human convalescent antiserum to human HS66 (GII-4) 1:25,600 1:6,400 1:25,600 1:25,600 1:6,400 1:6,400
LL616: pig convalescent-phase antiserum to porcine QW126 (QW101-like, GII-18)† 1:100 1:800 1:400 1:400 1:400 1:10
LL368 (negative control): preinoculation serum‡ <1:10 <1:10 <1:10 <1:10 <1:10 <1:10
MM982 (negative control): preinoculation serum‡ <1:10 <1:10 <1:10 <1:10 <1:10 <1:10

*NoV, norovirus; VLP, viruslike particle; ELISA, enzyme-linked immunosorbent assay.

†The QW126 shared 99% and 100% amino acid identities to the QW101 strain (GII-18) for a 169-bp segment in the RNA-dependent RNA polymerase region and a 363-bp segment in the capsid region, respectively.

‡LL368 and MM982 were sera from 2 gnotobiotic pigs before inoculation with porcine NoVs.

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