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Volume 11, Number 2—February 2005
Research

Carbapenemase-producing Enterobacteriaceae, U.S. Rivers

Cécile Aubron*, Laurent Poirel*, Ronald J Ash†, and Patrice Nordmann*Comments to Author 
Author affiliations: *University Paris XI, Paris, France; †Washburn University, Topeka, Kansas, USA

Main Article

Table 2

MICs (mg/L) of β-lactams for several carbapenemase producers and reference strain Escherichia coli DH10B

β-Lactam(s)* Enterobacter asburiae MS7† E. cloacae 1413B† Escherichia coli DH10B (pNat)‡ E. coli DH10B 
(pIMI-2)‡ E. coli DH10B
Amoxicillin >512 >512 >512 >512 4
Amoxicillin + CLA >512 >512 >512 >512 4
Ticarcillin 128 >256 128 256 4
Ticarcillin + CLA 16 >256 16 32 4
Piperacillin 16 >256 8 128 2
Piperacillin + TZB 4 >256 2 16 2
Cephalothin 512 >256 64 512 4
Cefotaxime 0.06 1 0.06 1 0.06
Ceftazidime 0.12 2 0.06 0.5 0.25
Aztreonam 4 8 4 64 0.12
Imipenem >64 >64 16 >64 0.06
Meropenem 32 4 2 32 0.06

*CLA, clavulanic acid at a fixed concentration of 2 mg/L; TZB, tazobactam at a fixed concentration of 4 mg/L.
Enterobacter asburiae MS7 produces acquired β-lactamase IMI-2, whereas E. cloacae 1413B produces acquired β-lactamases TEM-1 and IMI-1 (5).
‡Natural plasmid pNat harbors the blaIMI-2 gene, whereas pIMI-2 is a recombinant plasmid that has the same β-lactamase gene.

Main Article

References
  1. Lee  EH, Nicolas  MH, Kitzis  MD, Pialoux  G, Collatz  E, Gutmann  L. Association of two resistance mechanisms in a clinical isolate of Enterobacter cloacae with high level resistance to imipenem. Antimicrob Agents Chemother. 1991;35:10938.PubMed
  2. Nordmann  P, Poirel  L. Emerging carbapenemases in gram-negative aerobes. Clin Microbiol Infect. 2002;8:32131. DOIPubMed
  3. De Champs  C, Henquell  C, Guelon  D, Sirot  D, Gazuy  N, Sirot  J. Clinical and bacteriological study of nosocomial infections due to Enterobacter aerogenes resistant to imipenem. J Clin Microbiol. 1993;31:1237.PubMed
  4. Nordmann  P, Mariotte  S, Naas  T, Labia  R, Nicolas  MH. Biochemical properties of a carbapenem-hydrolyzing β-lactamase from Enterobacter cloacae and cloning of the gene into Escherichia coli. Antimicrob Agents Chemother. 1993;37:93946.PubMed
  5. Rasmussen  BA, Bush  K, Keeney  D, Yang  Y, Hare  R, O’Gara  C, Characterization of IMI-1 β-lactamase, a class A carbapenem-hydrolyzing enzyme from Enterobacter cloacae. Antimicrob Agents Chemother. 1996;40:20806.PubMed
  6. Naas  T, Livermore  DM, Nordmann  P. Characterization of an LysR family protein, SmeR from Serratia marcescens S6, its effect on expression of the carbapenem-hydrolyzing β-lactamase Sme-1, and comparison of this regulator with other β-lactamase regulators. Antimicrob Agents Chemother. 1995;39:62937.PubMed
  7. Naas  T, Vandel  L, Sougakoff  W, Livermore  DM, Nordmann  P. Cloning and sequence analysis of the gene for a carbapenem-hydrolyzing class A β-lactamase, Sme-1, from Serratia marcescens S6. Antimicrob Agents Chemother. 1994;38:126270.PubMed
  8. Naas  T, Nordmann  P. Analysis of a carbapenem-hydrolyzing class A β-lactamase from Enterobacter cloacae and of its LysR-type regulatory protein. Proc Natl Acad Sci U S A. 1994;91:76937. DOIPubMed
  9. Queenan  AM, Torres-Vierra  C, Gold  HS, Carmeli  Y, Eliopoulos  GM, Moellering  RC Jr, SME-type carbapenem-hydrolyzing class A β-lactamases from geographically diverse Serratia marcescens strains. Antimicrob Agents Chemother. 2000;44:30359. DOIPubMed
  10. Ambler  RP, Coulson  AF, Frère  JM, Ghuyssen  JM, Joris  B, Forsman  M, A standard numbering scheme for the class A beta-lactamase. Biochem J. 1991;276:26972.PubMed
  11. Miriagou  V, Tzouvelekis  LS, Rossiter  S, Tzelepi  E, Angulo  FJ, Whichard  JM. Imipenem resistance in Salmonella clinical strain due to plasmid-mediated class A carbapenemase KPC-2. Antimicrob Agents Chemother. 2003;47:1297300. DOIPubMed
  12. Yigit  H, Queenan  AM, Anderson  GJ, Domenech-Sanchez  A, Biddle  JW, Steward  CD, Novel carbapenem-hydrolyzing β-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae. Antimicrob Agents Chemother. 2001;45:115161. DOIPubMed
  13. Smith-Moland  E, Black  JA, Ourada  J, Reisbig  MD, Hanson  ND, Thomson  KS. Occurrence of newer β-lactamases in Klebsiella pneumoniae isolates from 24 U.S. hospitals. Antimicrob Agents Chemother. 2002;46:383742. DOIPubMed
  14. Smith-Moland  E, Hanson  ND, Herrera  VL, Black  AJ, Lockhart  T, Hossain  A, Plasmid-mediated, carbapenem-hydrolysing β-lactamase, KPC-2, in Klebsiella pneumoniae isolates. J Antimicrob Chemother. 2003;51:7114. DOIPubMed
  15. Poirel  L, Weldhagen  GF, Naas  T, De Champs  C, Dove  MG, Nordmann  P. GES-2, class A β-lactamase from Pseudomonas aeruginosa with increased hydrolysis of imipenem. Antimicrob Agents Chemother. 2001;45:2598603. DOIPubMed
  16. Sherwood  L. Antimicrobial use in animal feed—time to stop. N Engl J Med. 2001;345:12023. DOIPubMed
  17. White  DG, Shaohua  Z, Sudler  R, Sherry  A, Friedman  S, Chen  S, The isolation of antibiotic-resistant salmonella from retail ground meats. N Engl J Med. 2001;345:114754. DOIPubMed
  18. Mac Arthur  JV, Tuckfield  RC. Spatial patterns in antibiotic resistance among stream bacteria; effect of industrial pollution. Appl Environ Microbiol. 2000;66:37226. DOIPubMed
  19. Munesia  M, Garcia  A, Miro  E, Prats  G, Jofre  J, Navarro  F. Bacteriophages and diffusion of β-lactamase genes. Emerg Infect Dis. 2004;10:11347.PubMed
  20. Goni-Urriza  M, Capdepuy  M, Arpin  C, Raymond  N, Caumette  P, Quentin  C. Impact of an urban effluent on antibiotic resistance of riverine Enterobacteriaceae and Aeromonas spp. Appl Environ Microbiol. 2000;66:12532. DOIPubMed
  21. Baya  AM, Brayton  PR, Brown  VL, Grimes  DJ, Russek-Cohen  E, Colwell  RR. Coincident plasmids and antimicrobial resistance in marine bacteria isolated from polluted and unpolluted Atlantic Ocean samples. Appl Environ Microbiol. 1986;51:128592.PubMed
  22. Ash  RJ, Mauck  B, Morgan  M. Antibiotic resistance of gram-negative bacteria in rivers, United States. Emerg Infect Dis. 2002;8:7136.PubMed
  23. Brenner  DJ, McWorther  AC, Kai  A, Steigerwalt  AG, Farmer  JJ. Enterobacter asburiae sp. nov. a new species found in clinical specimens and reassignement of Erwinia dissolvens and Erwinia nimipressuralis to the genus Enterobacter as Enterobacter dissolvens comb. nov. and Enterobacter nimipressuralis comb. nov. J Clin Microbiol. 1986;23:111420.PubMed
  24. Avidor  B, Kletter  Y, Abulafia  S, Golan  Y, Ephros  M, Giladi  M. Molecular diagnosis of cat scratch disease: a two-step approach. J Clin Microbiol. 1997;35:192430.PubMed
  25. National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 6th ed. Approved standard M7-A5. Wayne (PA): The Committee; 2003.
  26. Poirel  L, Guibert  M, Bellais  S, Naas  T, Nordmann  P. Integron- and carbenicillinase-mediated reduced susceptibility to amoxicillin-clavulanic acid in isolates of multidrug-resistant Salmonella enterica serotype Typhimurium DT104 from French patients. Antimicrob Agents Chemother. 1999;43:1098104.PubMed
  27. Poirel  L, Naas  T, Guibert  M, Chaibi  EB, Labia  R, Nordmann  P. Molecular and biochemical characterization of VEB-1, a novel class A extended-spectrum β-lactamase encoded by an Escherichia coli integron gene. Antimicrob Agents Chemother. 1999;43:57381.PubMed
  28. Nazarowec-White  M, Farber  JM. Phenotypic and genotypic typing food and clinical isolates of Enterobacter sakazakii. J Med Microbiol. 1999;48:55967. DOIPubMed
  29. Telenius  H, Carter  NP, Bebb  CE, Nordenskjold  M, Ponder  BA, Tunnacliffe  A. Degenerate oligonucleotide-primer PCR: general amplification of target DNA by single degenerate primer. Genomics. 1992;13:71825. DOIPubMed
  30. Kieser  T. Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid. 1984;12:1936. DOIPubMed
  31. Danel  F, Hall  LM, Gur  D, Livermore  DM. OXA-14, another extended-spectrum variant of OXA-10 (PSE-2) β-lactamase from Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1995;39:18814.PubMed
  32. Churchward  G, Belin  D, Nagamine  Y. A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors. Gene. 1984;31:16571. DOIPubMed
  33. Michiels  T, Cornelis  G, Ellis  K, Grinsted  J. Tn2501, a component of the lactose transposon Tn951, is an example of a new category of class II transposable elements. J Bacteriol. 1987;169:62431.PubMed
  34. Pottumarthy  S, Smith-Moland  E, Juretschko  S, Swanzy  SR, Thomson  KS, Fritsche  TR. NmcA carbapenem-hydrolyzing enzyme in Enterobacter cloacae in North America. Emerg Infect Dis. 2003;9:9991002.PubMed
  35. Mimoz  O, Léotard  S, Jacolot  A, Padoin  C, Louchahi  K, Petitjean  O, Efficacies of imipenem, meropenem, cefepime, and ceftazidime in rats with experimental pneumonia due to a carbapenem-hydrolyzing β-lactamase-producing strain of Enterobacter cloacae. Antimicrob Agents Chemother. 2000;44:88590. DOIPubMed

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Page created: April 26, 2011
Page updated: April 26, 2011
Page reviewed: April 26, 2011
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