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Volume 11, Number 2—February 2005


Diagnostic System for Rapid and Sensitive Differential Detection of Pathogens

Thomas Briese*1, Gustavo Palacios*1, Mark Kokoris†1, Omar Jabado*, Zhiqiang Liu*, Neil Renwick*, Vishal Kapoor*, Inmaculada Casas‡, Francisco Pozo‡, Ron Limberger§, Pilar Perez-Brena‡, Jingyue Ju*, and W. Ian Lipkin*Comments to Author 
Author affiliations: *Columbia University, New York, New York, USA; †Qiagen Inc., Valencia, California, USA; ‡Instituto de Salud Carlos III, Majadahonda, Madrid, Spain; §New York State Department of Health, Albany, New York, USA

Main Article

Table 1

Sensitivity of pathogen detection by Mass Tag polymerase chain reaction determined by using plasmid and synthetic RNA standards*

Pathogen or protein Detection threshold (DNA copies/RNA copies)
Influenza A matrix 100/1,000
Influenza A N1 100/NA
Influenza A N2 100/NA
Influenza A H1 100/NA
Influenza A H2 100/NA
Influenza A H3 100/NA
Influenza A H5 100/NA
Influenza B H 500/1,000
RSV group A 100/1,000
RSV group B 100/500
Metapneumovirus 100/1,000
CoV-SARS 100/500
CoV-OC43 100/500
CoV-229E 100/500
HPIV-1 100/1,000
HPIV-2 100/1,000
HPIV-3 100/500
Chlamydia pneumoniae 100/NA
Mycoplasma pneumoniae 100/NA
Legionella pneumophila 100/NA
Enterovirus (genus) 500/1,000
Adenovirus (genus) 5,000/NA

*NA, not assessed; RSV, respiratory syncytial virus; CoV, coronavirus; SARS, severe acute respiratory syndrome; HPIV, human parainfluenza virus.

Main Article

1These authors contributed equally to this study.