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Volume 11, Number 2—February 2005


Diagnostic System for Rapid and Sensitive Differential Detection of Pathogens

Thomas Briese*1, Gustavo Palacios*1, Mark Kokoris†1, Omar Jabado*, Zhiqiang Liu*, Neil Renwick*, Vishal Kapoor*, Inmaculada Casas‡, Francisco Pozo‡, Ron Limberger§, Pilar Perez-Brena‡, Jingyue Ju*, and W. Ian Lipkin*Comments to Author 
Author affiliations: *Columbia University, New York, New York, USA; †Qiagen Inc., Valencia, California, USA; ‡Instituto de Salud Carlos III, Majadahonda, Madrid, Spain; §New York State Department of Health, Albany, New York, USA

Main Article

Table 2

Multiplex pathogen detection by Mass Tag polymerase chain reaction using Masscode-labeled primers in a 30-plex assay with clinical specimens with previously identified pathogens*

Pathogen No. positive/no. tested†
RSV A 2/2
RSV B 3/3
HPIV-1 1/1
HPIV-3 2/2
HPIV-4 2/2
CoV-SARS 4/4
Metapneumovirus 2/3
Influenza B 1/3
Influenza A 2/6
Adenovirus 2/2
Enterovirus 2/2

*RSV, respiratory syncytial virus; HPIV, human parainfluenza virus; CoV, coronavirus; SARS, severe acute respiratory syndrome.
†No. positive and consistent with previous diagnosis/number tested (with respective previous diagnosis).

Main Article

1These authors contributed equally to this study.