Volume 11, Number 3—March 2005
Dispatch
Identifying Relapsing Fever Borrelia, Senegal
Hamoud Brahim*, Jean David Perrier-Gros-Claude*, Danielle Postic†, Guy Baranton†, and Ronan Jambou*
Table
Primers used for amplification of the Borrelia 16S ribosomal RNA gene based on GenBank accession no. M88329 for Crocidurae
| Primer (reference) | Sequence | Tm, °C* | Nucleotide position |
|---|---|---|---|
| Fd3 (4) | AGAGTTTGATCCTGGCTTAG | 58 | 8–27 |
| T50 (4) | GTTACGACTTCACCCCCCT | 60 | 1478–1499 |
| Rec4 (4) | ATGCTAGAAACTGCATGA | 50 | 659–675 |
| Rec9 (4) | TCGTCTGAGTCCCCATCT | 56 | 1191–1174 |
| Fd4 | GGCTTAGAACTAACGCTGGCAG | 68 | 21–42 |
| 595R | CTTGCATATCCGCCTACTCA | 60 | 621–602 |
| 500R (4) | CTGCTGGCACGTAATTAGCC | 64 | 548–529 |
| BcroF | CGTCTTAAGCATGCAAGTCAG | 62 | 45–65 (U42283) |
| BdutF | CGTCTTAAGCATGCAAGTCAA | 60 | 45–65 (AF107364) |
| BrecF | GAAAGGAAGCCTTTAAAGCTTT | 60 | 193–214 (AF10362) |
| 255R |
CCCTACCAACTAGCTAATAAGACGC |
74 |
255–231 |
| *Tm, melting temperature. | |||
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